Alae (anatomy)

The alae is a protruding ridge that forms longitudinally on many nematodes. In the Caenorhabditis elegans nematode they are present in the L1, dauer (an alternative long living larvae stage where the nematode is dormant) and adult stages. The alae is most pronounced during the dauer larval stage and not present in the L2, and L3 C.elegans stages.

The term ‘alae’ is derived from ala, describing either one of the pair of ridges that forms on a nematode or an individual crease found on an individual ridge. The term ‘ala’ is rarely used in describing the alae and scientific journals use the term ‘alae’ both singularly and in the plural.

Structure

The alae is formed by the hypodermal seam cells where a fibrous ribbon of a ZP (zona pellucida) domain protein is produced. In C. elegans many of these proteins are termed CUT-1 (Ristoratore et al., 1994). The CUT refers to cuticulin which are the various proteins that are not solubilised by both reducing agents and detergents (Fujimoto and Kanaya, 1973; Boisvenue et al., 1991) made insoluble by the nature of there cross-links (Lewis et al., 1999; Politz and Philipp 1992; Lewis et al, 1994).

Described as a matrix that appears to be holding the two sides of the collagenous cuticle together for strengthening (De Giorgi, 1996) it should also be interpreted as a matrix separating the cuticle thereby exposing itself to the external environment (Vermont, 2003) . The alae is formed during an oxidative process where peroxidase acts on protein bound tyrosine residues. . The alae is a crease, that by the cross linking process causes radial shrinking of the seam cell secreted proteins.

Function

The function of the alae is not yet clear. It is generally given a function related to cuticle strength, nematode movement or fat storage. But the predominant structure of the C. elegans alae contain the ZP domain proteins (CUT-1, CUT-3, CUT-5). The ZP domain had been termed ‘the sequence in search of a function’ and has been given the functional role of matrix assembly and also putatively, functions in pheromone and olfactory signal transduction (Sutton et al., 2002; Matsushita et al., 1999). Despite the structural nature of the ZP domain, it is not the ideal protein for strength alone. Wherever ZP domains are found, they are found in definite or putative association with signal transduction accompanied by interaction with an external or hostile environment.

The continued growing list of ZP domain proteins that have an association with signal transduction appear to be revealing an ancient matrix that forms a protective barrier to an external environment whilst performing signal transduction in response to specific stimuli.

Function based on Genetic Data of the H. Contortus Dauer Nematode.

In the parasitic nematode Haemonchus contortus a ZP domain protein of a dauer stage nematode has been genetically associated to a target molecule found in the environment that this nematode could be using for exiting the dauer stage. This was statistically related with a high degree of significance indicating that the alae may specifically function as a receptor site (Vermont, 2003). In the dauer state the nematode is waiting for a signal that will indicate that conditions are suitable for egg survival. Upon receiving this signal they exit this state, progress to an adult and commence laying eggs.

In the case of H. contortus the dauer nematode can remain in the gastric epithelium for months and until the right signals indicating conditions outside the host are favorable for egg survival, it will then trigger resumption of development. During the dauer stage the mouth and anal openings are sealed and neural receptors around the head retracted (Royaie, 1998). The alae is more pronounced than at any other stage and remains exposed to the external environment.

Triggers for exiting the dauer state may be determined by the concentration of target molecules around the nematode. In order to measure concentrations accurately, a very large receptor area is necessary, hence a structure such as the alae may be required.

This type of signal transduction may be a common ZP domain protein function and is found in many structures. In some insects it has been found in the wings. The sight of an insect performing a set of zig zags before zooming off towards its target often up to half a kilometer or more away is not uncommon and mathematically impossible using the small head receptors alone. A homologous ZP domain protein in the bee and mosquito has been putatively associated to nectar. Another in the mosquito has some association to a protein in the outer layer of simple algae (Vermont, 2004) .

The Postulated Function

The alae appears to be a neural receptor responsive to just a handful of molecules particular to each species of nematode. Up to half a dozen types of receptors may be present at any one time on the alae and each type would be very numerous. The target molecules in the environment that stimulate each type of receptor may then be measured for their concentration and a threshold reached before an action is instigated. These actions may instigate entry into the dauer state (L1 alae), exiting of the dauer state (pheromone and or presence of an indicator for food availability), for sexual reproduction where the area around the vulva and gonads of the female and male (Raymond and Chow, 2000) use this family of receptors (RAM-5) where the nematode is likely to identify that reproductive organs are in contact. And quite probably there are some other functions that we haven’t yet found.

References

• Boisvenue, R. J. Stiff, M. I., Tonkinson, L. V. and Cox, G. N. (1991) Protective studies in sheep immunized with cuticular collagen proteins and peptides of Haemonchus contortus. Parasite Immunology,13, 227-240.

• Cox G., Kusch M. & Edgar R. 1981

• De Giorgi, C., De Luca, F., Di Vito, M., and Lamberti, F. (1997) Modulation of expression at the level of spiging of cut-1 RNA in the infective second-stage juvenile of the plant parasitic nematode Meloidogyne artiella. Mol. Gen. Genet. 253,589-598.

• Fujimoto, D., and Kanaya, S. (1973) Cuticlin a noncollagen structural protein form Ascaris cuticle, Arch. Biochem. Biophys. 157, 1-6.

• Lewis, E., sebastiano, M., Nola, M., Zei, F., Lassandro, F., Cermola, M., Favre, R., and Bazzicalupo, P., (1994) Cuticlin genes of nematodes. Parasite Biology and Biochemistry 1.1S p.57-58.

• Lewis, E., Hunter, S.J., Tetley, L., Nunes, C.P., Bazzicalupo, P., and Devaney, E., CUT-1 like genes are present in the filarial nematodes, Brugiapa Hangi and Brugia malayi, and, as in other nematodes, code for components of the cuticle, Molecular and Biochemical Parasitology 101 (1999) 173-183.

• Matsushita, F., Miyawaki, A., and Mikoshiba, K., (2000) Biochem. Biophys. Res. Commun. 268, 275-281

• Matsushita, F., Miyawaki, A., and Mikoshiba, K., Vomeroglandin/CRP-Ductin is strongly expressed in the glands associated with the mouse Vomeronasal Organ: Identification and Characterization of Mouse Vomeroglandin, Biochem. Biophys. Res. Commun. 268, 275-281 (2000)

• Raymond, Y.L. Yu, Nguyen, C., Hall, d., Chow, K.L. (2000) Expression of ram-5 the structural cell is required for ray morphogenesis in C. elegans mail tail. The EMBO Journal, V.19 N.14 3542-3555.

• Ristoratore, F., Cermola, M., Nola, M., Bazzicalupo, P., and Favre, R. (1994) Uultrastructural immuno localization of CUT-1 and CUT-2 antigenic sites in the cuticles of the nematode Caenorhabditis elegans, J. Submier. Cytol. Pathol. 26, 437-443.

• Sutton, K.A., Jungnickel, M.K., and Florman, H.M., (2002) If music be the food of love, Nature cell biology Vol.4, June.

• Vermont, R.C., (2003) Characterisation of cDNA from Haemonchous contortus (Barbers pole worm) encoding a Cuticulin. Dessitation, Charles Sturt University NSW. vermontrobert@yahoo.com.au

• Vermont, R.C., (2004) Unpublished (Patent). vermontrobert@yahoo.com.au