Cyclic adenosine monophosphate

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Overview

Cyclic adenosine monophosphate (cAMP, cyclic AMP or 3'-5'-cyclic adenosine monophosphate) is a molecule that is important in many biological processes; it is derived from what is known as adenosine triphosphate (ATP).

Synthesis and decomposition

cAMP is synthesised from ATP by adenylate cyclase which is located at the cell membranes. Adenylate cyclase is activated by the hormones glucagon and epinepherine through the activation of adenylate cyclase stimulatory G (G_s)-coupled receptors and inhibited by agonists of adenylate cyclase inhibitory G (G_i)-protein coupled receptors. Liver adenylate cyclase responds more strongly to glucagon, and muscle adenylate cyclase responds more strongly to adrenaline.

cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase.

Functions

cAMP is a second messenger, used for intracellular signal transduction, such as transferring the effects of hormones like glucagon and adrenaline, which cannot get through the cell membrane. Its purposes include the activation of protein kinases and regulating the effects of adrenaline and glucagon. It is also used to regulate the passage of Ca²⁺ through ion channels.

In humans

Main article: function of cAMP-dependent protein kinase

Epinephrine (adrenaline) binds its receptor, that associates with an heterotrimeric G protein. The G protein associates with adenylate cyclase that converts ATP to cAMP, spreading the signal (more details...)

In humans, cyclic AMP works by activating protein kinase A (PKA, also known as cAMP-dependent protein kinase). This is normally inactive as a tetrameric holoenzyme, consisting of 2 catalytic and 2 regulatory units (C₂R₂), with the regulatory units blocking the catalytic centers of the catalytic units.

Cyclic AMP binds to specific locations on the regulatory units of the protein kinase, and causes dissociation between the regulatory and catalytic subunits, thus activating the catalytic units and enabling them to phosphorylate substrate proteins.

Further effects thus depends on cAMP-dependent protein kinase, which are found in function of cAMP-dependent protein kinase. These effects depend on the type of cell, but includes regulation of glycogen, sugar, and lipid metabolism.

In non-humans

Role of cAMP in bacteria

In bacteria, the level of cAMP varies depending on the medium used for growth. In particular, cAMP is low when glucose is the carbon source. This occurs through inhibition of the cAMP-producing enzyme, adenylate cyclase, as a side effect of glucose transport into the cell. The transcription factor cAMP receptor protein (CRP) also called: CAP (Catabolite gene Activator Protein) forms a complex with cAMP and thereby is activated to bind to DNA. CRP-cAMP increases expression of a large number of genes, including some encoding enzymes that can supply energy independent of glucose.

An example of cAMP's function is the positive regulation of the lac operon. In an environment of a low glucose concentration, cAMP accumulates and binds to the allosteric site on CRP (cAMP receptor protein), a transcription activator protein. The protein assumes its active shape and binds to a specific site beside the lac promoter, making it easier for RNA polymerase to bind to the adjacent promoter to start transcription of the lac operon, increasing the rate of lac operon transcription. With a high glucose concentration, the cAMP concentration decreases, and the CRP disengages from the lac operon.

Role of cAMP in some slime molds

In the species Dictyostelium discoideum specifically, the chemotactic movement of cells are organized by periodic waves of cAMP that propagate through the cell. The waves are the result of a regulated production and secretion of extracellular cAMP and a spontaneous biological oscillator that initiates the waves at centers of territories.

Pathology

Role of cAMP in human carcinoma

Some research has suggested that a deregulation of cAMP pathways and an aberrant activation of cAMP-controlled genes is linked to the growth of some cancers.

Role of cAMP in Prefrontal Cortex Disorders

Recent research may indicate that cAMP affects the function of higher order thinking in the prefrontal cortex through its regulation of ion channels called hyperpolarization-activated cyclic nucleotide-gated channels (HCN). When cAMP stimulates the HCN, these gates open, rendering the brain cell closed to communication, thus interfering with prefrontal cortex function. This research is of interest to scientists studying the brain, especially the degradation of higher cognitive function in ADHD and aging.

See also

• Cyclic guanosine monophosphate (cGMP)
• Acrasin Specific to chemotactic use in Dictyostelium discoideum.

Additional images

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  cAMP represented in three ways

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  Adenosine triphosphate