View source for MBD1

{{Infobox_gene}}
'''Methyl-CpG-binding domain protein 1''' is a [[protein]] that in humans is encoded by the ''MBD1'' [[gene]].<ref name="pmid9207790">{{cite journal | vauthors = Cross SH, Meehan RR, Nan X, Bird A | title = A component of the transcriptional repressor MeCP1 shares a motif with DNA methyltransferase and HRX proteins | journal = Nat Genet | volume = 16 | issue = 3 | pages = 256–9 | date = Jul 1997 | pmid = 9207790 | pmc =  | doi = 10.1038/ng0797-256 }}</ref><ref name="pmid10441743">{{cite journal | vauthors = Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A | title = Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes | journal = Mamm Genome | volume = 10 | issue = 9 | pages = 906–12 | date = Sep 1999 | pmid = 10441743 | pmc =  | doi = 10.1007/s003359901112 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: MBD1 methyl-CpG binding domain protein 1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4152| accessdate = }}</ref> The protein encoded by MBD1 binds to [[methylation|methylated]] sequences in [[DNA]], and thereby influences [[Transcription (genetics)|transcription]].  It binds to a variety of methylated sequences, and appears to mediate repression of gene expression.  It has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. H3K9me3 is a repressive modification.

== Function ==

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters.  Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus.  All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity.  MBD1 and MBD2 map very close to each other on chromosome 18q21.<ref name="entrez" />

== Interactions ==

MBD1 has been shown to [[Protein-protein interaction|interact]] with [[ATF7IP]],<ref name=pmid12665582>{{cite journal | vauthors = Fujita N, Watanabe S, Ichimura T, Ohkuma Y, Chiba T, Saya H, Nakao M | title = MCAF Mediates MBD1-Dependent Transcriptional Repression | journal = Mol. Cell. Biol. | volume = 23 | issue = 8 | pages = 2834–43 | date = Apr 2003 | pmid = 12665582 | pmc = 152570 | doi = 10.1128/MCB.23.8.2834-2843.2003 }}</ref> [[CBX5 (gene)|CBX5]],<ref name=pmid12711603>{{cite journal | vauthors = Fujita N, Watanabe S, Ichimura T, Tsuruzoe S, Shinkai Y, Tachibana M, Chiba T, Nakao M | title = Methyl-CpG binding domain 1 (MBD1) interacts with the Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression | journal = J. Biol. Chem. | volume = 278 | issue = 26 | pages = 24132–8 | date = Jun 2003 | pmid = 12711603 | doi = 10.1074/jbc.M302283200 }}</ref><ref name=pmid12697822>{{cite journal | vauthors = Reese BE, Bachman KE, Baylin SB, Rountree MR | title = The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1 | journal = Mol. Cell. Biol. | volume = 23 | issue = 9 | pages = 3226–36 | date = May 2003 | pmid = 12697822 | pmc = 153189 | doi = 10.1128/MCB.23.9.3226-3236.2003 }}</ref> [[CHAF1A]]<ref name=pmid12697822/> and [[SUV39H1]].<ref name=pmid12711603/>

== References ==
{{reflist}}

== Further reading ==
{{refbegin | 2}}
* {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1–2 | pages = 171–4 | year = 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
* {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1–2 | pages = 149–56 | year = 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
* {{cite journal | vauthors = Hendrich B, Bird A | title = Identification and Characterization of a Family of Mammalian Methyl-CpG Binding Proteins | journal = Mol. Cell. Biol. | volume = 18 | issue = 11 | pages = 6538–47 | year = 1998 | pmid = 9774669 | pmc = 109239 | doi =  }}
* {{cite journal | vauthors = Ueba T, Kaspar B, Zhao X, Gage FH | title = Repression of human fibroblast growth factor 2 by a novel transcription factor | journal = J. Biol. Chem. | volume = 274 | issue = 15 | pages = 10382–7 | year = 1999 | pmid = 10187827 | doi = 10.1074/jbc.274.15.10382 }}
* {{cite journal | vauthors = Fujita N, Takebayashi S, Okumura K, Kudo S, Chiba T, Saya H, Nakao M | title = Methylation-Mediated Transcriptional Silencing in Euchromatin by Methyl-CpG Binding Protein MBD1 Isoforms | journal = Mol. Cell. Biol. | volume = 19 | issue = 9 | pages = 6415–26 | year = 1999 | pmid = 10454587 | pmc = 84611 | doi =  }}
* {{cite journal | vauthors = Ohki I, Shimotake N, Fujita N, Nakao M, Shirakawa M | title = Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1 | journal = EMBO J. | volume = 18 | issue = 23 | pages = 6653–61 | year = 2000 | pmid = 10581239 | pmc = 1171728 | doi = 10.1093/emboj/18.23.6653 }}
* {{cite journal | vauthors = Ng HH, Jeppesen P, Bird A | title = Active Repression of Methylated Genes by the Chromosomal Protein MBD1 | journal = Mol. Cell. Biol. | volume = 20 | issue = 4 | pages = 1394–406 | year = 2000 | pmid = 10648624 | pmc = 85293 | doi = 10.1128/MCB.20.4.1394-1406.2000 }}
* {{cite journal | vauthors = Fujita N, Shimotake N, Ohki I, Chiba T, Saya H, Shirakawa M, Nakao M | title = Mechanism of Transcriptional Regulation by Methyl-CpG Binding Protein MBD1 | journal = Mol. Cell. Biol. | volume = 20 | issue = 14 | pages = 5107–18 | year = 2000 | pmid = 10866667 | pmc = 85960 | doi = 10.1128/MCB.20.14.5107-5118.2000 }}
* {{cite journal | vauthors = Saito M, Ishikawa F | title = The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2 | journal = J. Biol. Chem. | volume = 277 | issue = 38 | pages = 35434–9 | year = 2002 | pmid = 12124384 | doi = 10.1074/jbc.M203455200 }}
* {{cite journal | vauthors = Beyer KS, Blasi F, Bacchelli E, Klauck SM, Maestrini E, Poustka A | title = Mutation analysis of the coding sequence of the MECP2 gene in infantile autism | journal = Hum. Genet. | volume = 111 | issue = 4–5 | pages = 305–9 | year = 2002 | pmid = 12384770 | doi = 10.1007/s00439-002-0786-3 }}
* {{cite journal | vauthors = Patra SK, Patra A, Zhao H, Carroll P, Dahiya R | title = Methyl-CpG-DNA binding proteins in human prostate cancer: expression of CXXC sequence containing MBD1 and repression of MBD2 and MeCP2 | journal = Biochem. Biophys. Res. Commun. | volume = 302 | issue = 4 | pages = 759–66 | year = 2003 | pmid = 12646234 | doi = 10.1016/S0006-291X(03)00253-5 }}
* {{cite journal | vauthors = Fujita N, Watanabe S, Ichimura T, Ohkuma Y, Chiba T, Saya H, Nakao M | title = MCAF Mediates MBD1-Dependent Transcriptional Repression | journal = Mol. Cell. Biol. | volume = 23 | issue = 8 | pages = 2834–43 | year = 2003 | pmid = 12665582 | pmc = 152570 | doi = 10.1128/MCB.23.8.2834-2843.2003 }}
* {{cite journal | vauthors = Reese BE, Bachman KE, Baylin SB, Rountree MR | title = The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1 | journal = Mol. Cell. Biol. | volume = 23 | issue = 9 | pages = 3226–36 | year = 2003 | pmid = 12697822 | pmc = 153189 | doi = 10.1128/MCB.23.9.3226-3236.2003 }}
* {{cite journal | vauthors = Fujita N, Watanabe S, Ichimura T, Tsuruzoe S, Shinkai Y, Tachibana M, Chiba T, Nakao M | title = Methyl-CpG binding domain 1 (MBD1) interacts with the Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression | journal = J. Biol. Chem. | volume = 278 | issue = 26 | pages = 24132–8 | year = 2003 | pmid = 12711603 | doi = 10.1074/jbc.M302283200 }}
* {{cite journal | vauthors = Carney RM, Wolpert CM, Ravan SA, Shahbazian M, Ashley-Koch A, Cuccaro ML, Vance JM, Pericak-Vance MA | title = Identification of MeCP2 mutations in a series of females with autistic disorder | journal = Pediatr. Neurol. | volume = 28 | issue = 3 | pages = 205–11 | year = 2003 | pmid = 12770674 | doi = 10.1016/S0887-8994(02)00624-0 }}
* {{cite journal | vauthors = Georgel PT, Horowitz-Scherer RA, Adkins N, Woodcock CL, Wade PA, Hansen JC | title = Chromatin compaction by human MeCP2. Assembly of novel secondary chromatin structures in the absence of DNA methylation | journal = J. Biol. Chem. | volume = 278 | issue = 34 | pages = 32181–8 | year = 2003 | pmid = 12788925 | doi = 10.1074/jbc.M305308200 }}
* {{cite journal | vauthors = Watanabe S, Ichimura T, Fujita N, Tsuruzoe S, Ohki I, Shirakawa M, Kawasuji M, Nakao M | title = Methylated DNA-binding domain 1 and methylpurine–DNA glycosylase link transcriptional repression and DNA repair in chromatin | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 100 | issue = 22 | pages = 12859–64 | year = 2004 | pmid = 14555760 | pmc = 240709 | doi = 10.1073/pnas.2131819100 }}
{{refend}}

{{PDB Gallery|geneid=4152}}

[[Category:DNA]]