Lassa fever other diagnostic studies: Difference between revisions

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{{Lassa fever}}
{{Lassa fever}}
{{CMG}}; {{AE}} {{Ammu}}
{{CMG}}; {{AE}} {{Ammu}}
{{SK}} Lassa hemorrhagic fever; LHF
==Overview==
==Overview==
Other laboratory tests to confirm the diagnosis of lassa fever includes [[enzyme-linked immunosorbent serologic assays]] ([[ELISA]]), [[reverse transcription]]-[[polymerase chain reaction]] ([[RT-PCR]]) and [[immunohistochemistry]] of [[skin]], [[tissue]] and [[liver]] cells. [[ELISA test]] for [[antigen]] and [[IgM]] [[antibodies]] gives 88% [[sensitivity]] and 90% [[specificity]] for the presence of the infection. Lassa fever can also be found in [[cerebrospinal fluid]]. [[Immunohistochemistry]] performed on tissue specimens can be used to make a [[post mortem]] diagnosis. The virus can also be detected by [[reverse transcriptase polymerase chain reaction]] ([[RT-PCR]]); however, this method is primarily a research tool.<ref>Lassa Fever Encephalopathy: Lassa Virus in Cerebrospinal Fluid but Not in Serum
Other diagnostic tests to confirm the diagnosis of lassa fever include [[reverse transcription]]-[[polymerase chain reaction]] ([[RT-PCR]]) and [[immunohistochemistry]] using either [[skin]], [[tissue]] or [[liver]] tissue.
Stephan Günther, Boye Weisner, Andreas Roth, Thomas Grewing, Marcel Asper, Christian Drosten, Petra Emmerich, Jochen Petersen, Martin Wilczek and Herbert Schmitz
The Journal of Infectious Diseases , Vol. 184, No. 3 (Aug. 1, 2001), pp. 345-349</ref>


==Other Diagnostic Studies==
==Other Diagnostic Studies==
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| align="center" style="background:#4479BA; padding: 5px 5px;"|{{fontcolor|#FFF|'''Detects'''}}
| align="center" style="background:#4479BA; padding: 5px 5px;"|{{fontcolor|#FFF|'''Detects'''}}
|-
|-
| style="padding: 5px 5px; background: #EBEBEB;"| ELISA (Serology)||style="padding: 5px 5px; background: #EBEBEB;"|Serum or plasma ||style="padding: 5px 5px; background: #EBEBEB;"|Freeze or refrigerate||style="padding: 5px 5px; background: #EBEBEB;"|Detects Viral antigen, IgM and IgG antibody
| style="padding: 5px 5px; background: #EBEBEB;"| [[Polymerase Chain Reaction]] (PCR)||style="padding: 5px 5px; background: #EBEBEB;"|[[Serum]] or [[plasma]] or [[tissues]] (fresh frozen)  ||style="padding: 5px 5px; background: #EBEBEB;"|Freeze or refrigerate||style="padding: 5px 5px; background: #EBEBEB;"|Detects [[DNA]], [[RNA]] (genetic material) from [[virus]].
|-
| style="padding: 5px 5px; background: #EBEBEB;"| Polymerase Chain Reaction(PCR)||style="padding: 5px 5px; background: #EBEBEB;"|Serum or plasma or tissues (fresh frozen)  ||style="padding: 5px 5px; background: #EBEBEB;"|Freeze or refrigerate||style="padding: 5px 5px; background: #EBEBEB;"|Detects DNA, RNA (genetic material) from virus
|-
|-
| style="padding: 5px 5px; background: #EBEBEB;"| Immunohisto-chemistry||style="padding: 5px 5px; background: #EBEBEB;"|Liver biopsy from fatal cases ||style="padding: 5px 5px; background: #EBEBEB;"|Fix in formalin and store or transport in room temperature ||style="padding: 5px 5px; background: #EBEBEB;"|Detects viral antigen in cells
| style="padding: 5px 5px; background: #EBEBEB;"| [[Immunohistochemistry]]||style="padding: 5px 5px; background: #EBEBEB;"|[[Liver]] biopsy from fatal cases ||style="padding: 5px 5px; background: #EBEBEB;"|Fix in formalin and store or transport in room temperature ||style="padding: 5px 5px; background: #EBEBEB;"|Detects viral [[antigen]] in [[cells]].
|-
|-
| style="padding: 5px 5px; background: #EBEBEB;"| Immunohisto-chemistry||style="padding: 5px 5px; background: #EBEBEB;"|Tissue biopsy from fatal cases ||style="padding: 5px 5px; background: #EBEBEB;"|Fix in formalin and store or transport in room temperature ||style="padding: 5px 5px; background: #EBEBEB;"|Detects viral antigen in cells
| style="padding: 5px 5px; background: #EBEBEB;"| [[Immunohistochemistry]]||style="padding: 5px 5px; background: #EBEBEB;"|[[Tissue]] biopsy from fatal cases ||style="padding: 5px 5px; background: #EBEBEB;"|Fix in formalin and store or transport in room temperature ||style="padding: 5px 5px; background: #EBEBEB;"|Detects viral [[antigen]] in cells
|-
|-
| style="padding: 5px 5px; background: #EBEBEB;"| Immunohisto-chemistry||style="padding: 5px 5px; background: #EBEBEB;"|Skin biopsy from fatal cases ||style="padding: 5px 5px; background: #EBEBEB;"|Fix in formalin and store or transport in room temperature ||style="padding: 5px 5px; background: #EBEBEB;"|Detects viral antigen in cells
| style="padding: 5px 5px; background: #EBEBEB;"| [[Immunohistochemistry]]||style="padding: 5px 5px; background: #EBEBEB;"|[[Skin]] biopsy from fatal cases ||style="padding: 5px 5px; background: #EBEBEB;"|Fix in formalin and store or transport in room temperature ||style="padding: 5px 5px; background: #EBEBEB;"|Detects viral [[antigen]] in cells
|}
|}
<SMALL>Data from the Center of Disease Control and Prevention</SMALL>
<SMALL>Data from the Centers for Disease Control and Prevention</SMALL>
 
==References==
==References==
{{Reflist|2}}
{{Reflist|2}}
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[[Category:Tropical disease]]
[[Category:Tropical disease]]
[[Category:Biological weapons]]
[[Category:Biological weapons]]
[[Category:Infectious disease]]
 


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Latest revision as of 18:08, 18 September 2017

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Ammu Susheela, M.D. [2]

Overview

Other diagnostic tests to confirm the diagnosis of lassa fever include reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry using either skin, tissue or liver tissue.

Other Diagnostic Studies

Listed below are some of the diagnostic test used to confirm Lassa fever.[1]

Diagnostic Test Samples required Preparation & Storage Detects
Polymerase Chain Reaction (PCR) Serum or plasma or tissues (fresh frozen) Freeze or refrigerate Detects DNA, RNA (genetic material) from virus.
Immunohistochemistry Liver biopsy from fatal cases Fix in formalin and store or transport in room temperature Detects viral antigen in cells.
Immunohistochemistry Tissue biopsy from fatal cases Fix in formalin and store or transport in room temperature Detects viral antigen in cells
Immunohistochemistry Skin biopsy from fatal cases Fix in formalin and store or transport in room temperature Detects viral antigen in cells

Data from the Centers for Disease Control and Prevention

References

  1. ↑ "The Centers for Disease Control and Prevention" (PDF).


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