DDX20: Difference between revisions

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{{Infobox_gene}}
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'''Probable ATP-dependent RNA helicase DDX20''', also known as '''DEAD-box helicase 20''' and '''gem-associated protein 3''' ('''GEMIN3'''), is an [[enzyme]] that in humans is encoded by the ''DDX20'' [[gene]].<ref name="pmid10383418">{{cite journal | vauthors = Grundhoff AT, Kremmer E, Türeci O, Glieden A, Gindorf C, Atz J, Mueller-Lantzsch N, Schubach WH, Grässer FA | title = Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C | journal = J Biol Chem | volume = 274 | issue = 27 | pages = 19136–44 | date = July 1999 | pmid = 10383418 | pmc =  | doi = 10.1074/jbc.274.27.19136 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11218| accessdate = }}</ref>
| update_page = yes
| require_manual_inspection = no
| update_protein_box = yes
| update_summary = yes
| update_citations = yes
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<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
== Function ==
{{GNF_Protein_box
| image = PBB_Protein_DDX20_image.jpg
| image_source = [[Protein_Data_Bank|PDB]] rendering based on 2oxc.
| PDB = {{PDB2|2oxc}}
| Name = DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
| HGNCid = 2743
| Symbol = DDX20
| AltSymbols =; DKFZp434H052; DP103; GEMIN3
| OMIM = 606168
| ECnumber = 
| Homologene = 5214
| MGIid = 1858415
| GeneAtlas_image1 = PBB_GE_DDX20_gnf1h00107_at_tn.png
| Function = {{GNF_GO|id=GO:0000166 |text = nucleotide binding}} {{GNF_GO|id=GO:0003677 |text = DNA binding}} {{GNF_GO|id=GO:0004004 |text = ATP-dependent RNA helicase activity}} {{GNF_GO|id=GO:0005515 |text = protein binding}} {{GNF_GO|id=GO:0005524 |text = ATP binding}} {{GNF_GO|id=GO:0016564 |text = transcription repressor activity}} {{GNF_GO|id=GO:0016787 |text = hydrolase activity}}
| Component = {{GNF_GO|id=GO:0005634 |text = nucleus}} {{GNF_GO|id=GO:0005681 |text = spliceosome}} {{GNF_GO|id=GO:0005737 |text = cytoplasm}} {{GNF_GO|id=GO:0005856 |text = cytoskeleton}} {{GNF_GO|id=GO:0017053 |text = transcriptional repressor complex}}
| Process = {{GNF_GO|id=GO:0000122 |text = negative regulation of transcription from RNA polymerase II promoter}} {{GNF_GO|id=GO:0000244 |text = assembly of spliceosomal tri-snRNP}} {{GNF_GO|id=GO:0006397 |text = mRNA processing}} {{GNF_GO|id=GO:0008380 |text = RNA splicing}} {{GNF_GO|id=GO:0043065 |text = positive regulation of apoptosis}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 11218
    | Hs_Ensembl = ENSG00000064703
    | Hs_RefseqProtein = NP_009135
    | Hs_RefseqmRNA = NM_007204
    | Hs_GenLoc_db = 
    | Hs_GenLoc_chr = 1
    | Hs_GenLoc_start = 112099742
    | Hs_GenLoc_end = 112111722
    | Hs_Uniprot = Q9UHI6
    | Mm_EntrezGene = 53975
    | Mm_Ensembl = ENSMUSG00000027905
    | Mm_RefseqmRNA = NM_017397
    | Mm_RefseqProtein = NP_059093
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 3
    | Mm_GenLoc_start = 105806331
    | Mm_GenLoc_end = 105815630
    | Mm_Uniprot = Q059Z6
  }}
}}
'''DEAD (Asp-Glu-Ala-Asp) box polypeptide 20''', also known as '''DDX20''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11218| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
[[DEAD box]] proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative [[RNA helicase]]s. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and [[ribosome]] and [[spliceosome]] assembly. Based on their distribution patterns, some members of this family are believed to be involved in [[embryogenesis]], [[spermatogenesis]], and cellular growth and division. This gene encodes a DEAD box protein, which has an [[ATPase]] activity and is a component of the [[survival of motor neuron]] (SMN) complex.<ref name="entrez" /> This DEAD box protein is one of the gem-associated proteins (GEMIN3) that interacts directly with SMN.<ref name="Seng">{{cite journal|last1=Seng|first1=CO|last2=Magee|first2=C|last3=Young|first3=PJ|last4=Lorson|first4=CL|last5=Allen|first5=JP|title=The SMN structure reveals its crucial role in snRNP assembly.|journal=Human Molecular Genetics|date=15 April 2015|volume=24|issue=8|pages=2138–46|pmid=25561692|doi=10.1093/hmg/ddu734}}<!--|accessdate=27 May 2016--></ref>
{{PBB_Summary
SMN is the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.<ref name="entrez" />
| section_title =
| summary_text = DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.<ref name="entrez">{{cite web | title = Entrez Gene: DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11218| accessdate = }}</ref>
}}


==References==
== Biological Implication ==
{{reflist|2}}
Previous research has revealed that DDX20 may act as a tumor suppressor in [[hepatocellular carcinoma]] and as a [[tumor]] promoter in [[breast cancer]]. DDX20 deficiency enhances [[NF-κB]] activity by impairing the NF-κB-suppressive action of [[microRNA]]s, and suggest that dysregulation of the microRNA machinery components may also be involved in pathogenesis in various human diseases.<ref>{{Cite journal|last=Takata|first=Akemi|last2=Otsuka|first2=Motoyuki|last3=Yoshikawa|first3=Takeshi|last4=Kishikawa|first4=Takahiro|last5=Kudo|first5=Yotaro|last6=Goto|first6=Tadashi|last7=Yoshida|first7=Haruhiko|last8=Koike|first8=Kazuhiko|date=2012-04-13|title=A miRNA machinery component DDX20 controls NF-κB via microRNA-140 function|journal=Biochemical and Biophysical Research Communications|volume=420|issue=3|pages=564–569|doi=10.1016/j.bbrc.2012.03.034|issn=1090-2104|pmid=22445758}}</ref> Such as miRNA-140 which acts as a liver tumor suppressor, and due to a deficiency of DDX20, miRNA-140 function gets impair, this subsequent functional impairment of miRNAs could lead to hepatocarcinogenesis.Similarly,<ref>{{Cite journal|last=Takata|first=Akemi|last2=Otsuka|first2=Motoyuki|last3=Yoshikawa|first3=Takeshi|last4=Kishikawa|first4=Takahiro|last5=Hikiba|first5=Yohko|last6=Obi|first6=Shuntaro|last7=Goto|first7=Tadashi|last8=Kang|first8=Young Jun|last9=Maeda|first9=Shin|date=January 2013|title=MicroRNA-140 acts as a liver tumor suppressor by controlling NF-κB activity by directly targeting DNA methyltransferase 1 (Dnmt1) expression|journal=Hepatology|volume=57|issue=1|pages=162–170|doi=10.1002/hep.26011|issn=1527-3350|pmc=3521841|pmid=22898998}}</ref> DDX20 may promote the progression of [[Prostate cancer]] (PCa) through the NF-κB pathway.<ref>{{Cite journal|last=Chen|first=Weiguo|last2=Zhou|first2=Peng|last3=Li|first3=Xiaowei|date=June 2016|title=High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway|journal=International Journal of Molecular Medicine|volume=37|issue=6|pages=1551–1557|doi=10.3892/ijmm.2016.2575|issn=1791-244X|pmc=4866965|pmid=27121695}}</ref> In a clinical based study it has been observed that positive DP103/NF-κB feedback loop promotes constitutive NF-κB activation in invasive breast cancers and activation of this pathway is linked to cancer progression and the acquisition of [[chemotherapy]] resistance. It makes DP103 has potential as a therapeutic target for breast cancer treatment.<ref>{{Cite journal|last=Shin|first=Eun Myoung|last2=Hay|first2=Hui Sin|last3=Lee|first3=Moon Hee|last4=Goh|first4=Jen Nee|last5=Tan|first5=Tuan Zea|last6=Sen|first6=Yin Ping|last7=Lim|first7=See Wee|last8=Yousef|first8=Einas M.|last9=Ong|first9=Hooi Tin|date=September 2014|title=DEAD-box helicase DP103 defines metastatic potential of human breast cancers|journal=The Journal of Clinical Investigation|volume=124|issue=9|pages=3807–3824|doi=10.1172/JCI73451|issn=1558-8238|pmc=4151228|pmid=25083991}}</ref>
==Further reading==
 
== Interactions ==
 
DDX20 has been shown to [[Protein-protein interaction|interact]] with:
{{div col|colwidth=20em}}
* [[EIF2C2]],<ref name = pmid11914277/><ref name = pmid14970384>{{cite journal | vauthors = Nelson PT, Hatzigeorgiou AG, Mourelatos Z | title = miRNP:mRNA association in polyribosomes in a human neuronal cell line | journal = RNA | volume = 10 | issue = 3 | pages = 387–94 | date = March 2004 | pmid = 14970384 | pmc = 1370934 | doi =  10.1261/rna.5181104}}</ref>
* [[GEMIN4]],<ref name = pmid11914277>{{cite journal | vauthors = Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G | title = miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs | journal = Genes Dev. | volume = 16 | issue = 6 | pages = 720–8 | date = March 2002 | pmid = 11914277 | pmc = 155365 | doi = 10.1101/gad.974702 }}</ref><ref name = pmid12668731>{{cite journal | vauthors = Carnegie GK, Sleeman JE, Morrice N, Hastie CJ, Peggie MW, Philp A, Lamond AI, Cohen PT | title = Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs | journal = J. Cell Sci. | volume = 116 | issue = Pt 10 | pages = 1905–13 | date = May 2003 | pmid = 12668731 | doi = 10.1242/jcs.00409 }}</ref><ref name = pmid10725331>{{cite journal | vauthors = Charroux B, Pellizzoni L, Perkinson RA, Yong J, Shevchenko A, Mann M, Dreyfuss G | title = Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli | journal = J. Cell Biol. | volume = 148 | issue = 6 | pages = 1177–86 | date = March 2000 | pmid = 10725331 | pmc = 2174312 | doi =  10.1083/jcb.148.6.1177}}</ref>
* [[GEMIN5]],<ref name = pmid11914277/><ref name = pmid11714716>{{cite journal | vauthors = Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G | title = Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins | journal = J. Biol. Chem. | volume = 277 | issue = 7 | pages = 5631–6 | date = February 2002 | pmid = 11714716 | doi = 10.1074/jbc.M109448200 }}</ref>
* [[LSM2]],<ref name = pmid10601333>{{cite journal | vauthors = Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G | title = Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems | journal = J. Cell Biol. | volume = 147 | issue = 6 | pages = 1181–94 | date = December 1999 | pmid = 10601333 | pmc = 2168095 | doi =  10.1083/jcb.147.6.1181}}</ref>
* [[SMN1]],<ref name = pmid11914277/><ref name = pmid12668731/><ref name = pmid10601333/><ref name = pmid10942426>{{cite journal | vauthors = Meister G, Bühler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U | title = Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins | journal = Hum. Mol. Genet. | volume = 9 | issue = 13 | pages = 1977–86 | date = August 2000 | pmid = 10942426 | doi =  10.1093/hmg/9.13.1977}}</ref>
* [[SNRPB]],<ref name = pmid10601333/>
* [[SNRPD3]]<ref name = pmid10601333/> and
* [[SNRPG]],<ref name = pmid10601333/>
* [[Small nuclear ribonucleoprotein D1|SNRPD1]],<ref name = pmid10601333/>
* [[Small nuclear ribonucleoprotein D2|SNRPD2]],<ref name = pmid10601333/>
* [[Small nuclear ribonucleoprotein polypeptide E|SNRPE]],<ref name = pmid10601333/>
* [[Small nuclear ribonucleoprotein polypeptide F|SNRPF]],<ref name = pmid10601333/>  and
* [[Survival of motor neuron protein-interacting protein 1|SIP1]].<ref name = pmid11914277/><ref name = pmid10601333/>
{{Div col end}}
 
== References ==
{{reflist}}
 
== Further reading ==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
* {{cite journal | vauthors = Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA | title = A "double adaptor" method for improved shotgun library construction. | journal = Anal. Biochem. | volume = 236 | issue = 1 | pages = 107–13 | year = 1996 | pmid = 8619474 | doi = 10.1006/abio.1996.0138 }}
| citations =
* {{cite journal | vauthors = Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA | title = Large-scale concatenation cDNA sequencing. | journal = Genome Res. | volume = 7 | issue = 4 | pages = 353–8 | year = 1997 | pmid = 9110174 | pmc = 139146 | doi = 10.1101/gr.7.4.353 }}
*{{cite journal | author=Andersson B, Wentland MA, Ricafrente JY, ''et al.'' |title=A "double adaptor" method for improved shotgun library construction. |journal=Anal. Biochem. |volume=236 |issue= 1 |pages= 107-13 |year= 1996 |pmid= 8619474 |doi= 10.1006/abio.1996.0138 }}
* {{cite journal | vauthors = Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G | title = Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. | journal = J. Cell Biol. | volume = 147 | issue = 6 | pages = 1181–94 | year = 2000 | pmid = 10601333 | pmc = 2168095 | doi = 10.1083/jcb.147.6.1181 }}
*{{cite journal | author=Yu W, Andersson B, Worley KC, ''et al.'' |title=Large-scale concatenation cDNA sequencing. |journal=Genome Res. |volume=7 |issue= 4 |pages= 353-8 |year= 1997 |pmid= 9110174 |doi= }}
* {{cite journal | vauthors = Charroux B, Pellizzoni L, Perkinson RA, Yong J, Shevchenko A, Mann M, Dreyfuss G | title = Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. | journal = J. Cell Biol. | volume = 148 | issue = 6 | pages = 1177–86 | year = 2000 | pmid = 10725331 | pmc = 2174312 | doi = 10.1083/jcb.148.6.1177 }}
*{{cite journal  | author=Grundhoff AT, Kremmer E, Türeci O, ''et al.'' |title=Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C. |journal=J. Biol. Chem. |volume=274 |issue= 27 |pages= 19136-44 |year= 1999 |pmid= 10383418 |doi= }}
* {{cite journal | vauthors = Meister G, Bühler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U | title = Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. | journal = Hum. Mol. Genet. | volume = 9 | issue = 13 | pages = 1977–86 | year = 2000 | pmid = 10942426 | doi = 10.1093/hmg/9.13.1977 }}
*{{cite journal | author=Charroux B, Pellizzoni L, Perkinson RA, ''et al.'' |title=Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. |journal=J. Cell Biol. |volume=147 |issue= 6 |pages= 1181-94 |year= 2000 |pmid= 10601333 |doi= }}
* {{cite journal | vauthors = Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G | title = A functional interaction between the survival motor neuron complex and RNA polymerase II. | journal = J. Cell Biol. | volume = 152 | issue = 1 | pages = 75–85 | year = 2001 | pmid = 11149922 | pmc = 2193649 | doi = 10.1083/jcb.152.1.75 }}
*{{cite journal | author=Charroux B, Pellizzoni L, Perkinson RA, ''et al.'' |title=Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. |journal=J. Cell Biol. |volume=148 |issue= 6 |pages= 1177-86 |year= 2000 |pmid= 10725331 |doi= }}
* {{cite journal | vauthors = Friesen WJ, Paushkin S, Wyce A, Massenet S, Pesiridis GS, Van Duyne G, Rappsilber J, Mann M, Dreyfuss G | title = The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins. | journal = Mol. Cell. Biol. | volume = 21 | issue = 24 | pages = 8289–300 | year = 2001 | pmid = 11713266 | pmc = 99994 | doi = 10.1128/MCB.21.24.8289-8300.2001 }}
*{{cite journal | author=Meister G, Bühler D, Laggerbauer B, ''et al.'' |title=Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. |journal=Hum. Mol. Genet. |volume=9 |issue= 13 |pages= 1977-86 |year= 2000 |pmid= 10942426 |doi= }}
* {{cite journal | vauthors = Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G | title = Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. | journal = J. Biol. Chem. | volume = 277 | issue = 7 | pages = 5631–6 | year = 2002 | pmid = 11714716 | doi = 10.1074/jbc.M109448200 }}
*{{cite journal | author=Pellizzoni L, Charroux B, Rappsilber J, ''et al.'' |title=A functional interaction between the survival motor neuron complex and RNA polymerase II. |journal=J. Cell Biol. |volume=152 |issue= 1 |pages= 75-85 |year= 2001 |pmid= 11149922 |doi= }}
* {{cite journal | vauthors = Pellizzoni L, Baccon J, Rappsilber J, Mann M, Dreyfuss G | title = Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. | journal = J. Biol. Chem. | volume = 277 | issue = 9 | pages = 7540–5 | year = 2002 | pmid = 11748230 | doi = 10.1074/jbc.M110141200 }}
*{{cite journal  | author=Friesen WJ, Paushkin S, Wyce A, ''et al.'' |title=The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins. |journal=Mol. Cell. Biol. |volume=21 |issue= 24 |pages= 8289-300 |year= 2001 |pmid= 11713266 |doi= 10.1128/MCB.21.24.8289-8300.2001 }}
* {{cite journal | vauthors = Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G | title = miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. | journal = Genes Dev. | volume = 16 | issue = 6 | pages = 720–8 | year = 2002 | pmid = 11914277 | pmc = 155365 | doi = 10.1101/gad.974702 }}
*{{cite journal | author=Gubitz AK, Mourelatos Z, Abel L, ''et al.'' |title=Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. |journal=J. Biol. Chem. |volume=277 |issue= 7 |pages= 5631-6 |year= 2002 |pmid= 11714716 |doi= 10.1074/jbc.M109448200 }}
* {{cite journal | vauthors = Klappacher GW, Lunyak VV, Sykes DB, Sawka-Verhelle D, Sage J, Brard G, Ngo SD, Gangadharan D, Jacks T, Kamps MP, Rose DW, Rosenfeld MG, Glass CK | title = An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation. | journal = Cell | volume = 109 | issue = 2 | pages = 169–80 | year = 2002 | pmid = 12007404 | doi = 10.1016/S0092-8674(02)00714-6 }}
*{{cite journal | author=Pellizzoni L, Baccon J, Rappsilber J, ''et al.'' |title=Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. |journal=J. Biol. Chem. |volume=277 |issue= 9 |pages= 7540-5 |year= 2002 |pmid= 11748230 |doi= 10.1074/jbc.M110141200 }}
* {{cite journal | vauthors = Baccon J, Pellizzoni L, Rappsilber J, Mann M, Dreyfuss G | title = Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex. | journal = J. Biol. Chem. | volume = 277 | issue = 35 | pages = 31957–62 | year = 2002 | pmid = 12065586 | doi = 10.1074/jbc.M203478200 }}
*{{cite journal | author=Mourelatos Z, Dostie J, Paushkin S, ''et al.'' |title=miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. |journal=Genes Dev. |volume=16 |issue= 6 |pages= 720-8 |year= 2002 |pmid= 11914277 |doi= 10.1101/gad.974702 }}
* {{cite journal | vauthors = Narayanan U, Ospina JK, Frey MR, Hebert MD, Matera AG | title = SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta. | journal = Hum. Mol. Genet. | volume = 11 | issue = 15 | pages = 1785–95 | year = 2003 | pmid = 12095920 | pmc = 1630493 | doi = 10.1093/hmg/11.15.1785 }}
*{{cite journal | author=Klappacher GW, Lunyak VV, Sykes DB, ''et al.'' |title=An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation. |journal=Cell |volume=109 |issue= 2 |pages= 169-80 |year= 2002 |pmid= 12007404 |doi= }}
* {{cite journal | vauthors = Carnegie GK, Sleeman JE, Morrice N, Hastie CJ, Peggie MW, Philp A, Lamond AI, Cohen PT | title = Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs. | journal = J. Cell Sci. | volume = 116 | issue = Pt 10 | pages = 1905–13 | year = 2004 | pmid = 12668731 | doi = 10.1242/jcs.00409 }}
*{{cite journal | author=Baccon J, Pellizzoni L, Rappsilber J, ''et al.'' |title=Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex. |journal=J. Biol. Chem. |volume=277 |issue= 35 |pages= 31957-62 |year= 2002 |pmid= 12065586 |doi= 10.1074/jbc.M203478200 }}
* {{cite journal | vauthors = Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM | title = Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region. | journal = Genomics | volume = 83 | issue = 1 | pages = 153–67 | year = 2004 | pmid = 14667819 | doi = 10.1016/S0888-7543(03)00235-0 }}
*{{cite journal | author=Narayanan U, Ospina JK, Frey MR, ''et al.'' |title=SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta. |journal=Hum. Mol. Genet. |volume=11 |issue= 15 |pages= 1785-95 |year= 2003 |pmid= 12095920 |doi= }}
* {{cite journal | vauthors = Nelson PT, Hatzigeorgiou AG, Mourelatos Z | title = miRNP:mRNA association in polyribosomes in a human neuronal cell line. | journal = RNA | volume = 10 | issue = 3 | pages = 387–94 | year = 2004 | pmid = 14970384 | pmc = 1370934 | doi = 10.1261/rna.5181104 }}
*{{cite journal | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
* {{cite journal | vauthors = Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP | title = Large-scale characterization of HeLa cell nuclear phosphoproteins. | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 101 | issue = 33 | pages = 12130–5 | year = 2004 | pmid = 15302935 | pmc = 514446 | doi = 10.1073/pnas.0404720101 }}
*{{cite journal | author=Carnegie GK, Sleeman JE, Morrice N, ''et al.'' |title=Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs. |journal=J. Cell. Sci. |volume=116 |issue= Pt 10 |pages= 1905-13 |year= 2004 |pmid= 12668731 |doi= 10.1242/jcs.00409 }}
*{{cite journal | author=Lehner B, Semple JI, Brown SE, ''et al.'' |title=Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region. |journal=Genomics |volume=83 |issue= 1 |pages= 153-67 |year= 2004 |pmid= 14667819 |doi=  }}
*{{cite journal  | author=Ota T, Suzuki Y, Nishikawa T, ''et al.'' |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40-5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
*{{cite journal  | author=Nelson PT, Hatzigeorgiou AG, Mourelatos Z |title=miRNP:mRNA association in polyribosomes in a human neuronal cell line. |journal=RNA |volume=10 |issue= 3 |pages= 387-94 |year= 2004 |pmid= 14970384 |doi=  }}
*{{cite journal  | author=Beausoleil SA, Jedrychowski M, Schwartz D, ''et al.'' |title=Large-scale characterization of HeLa cell nuclear phosphoproteins. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=101 |issue= 33 |pages= 12130-5 |year= 2004 |pmid= 15302935 |doi= 10.1073/pnas.0404720101 }}
}}
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Latest revision as of 19:43, 8 November 2017

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Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

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Ensembl

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UniProt

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RefSeq (mRNA)

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RefSeq (protein)

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Location (UCSC)n/an/a
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Wikidata
View/Edit Human

Probable ATP-dependent RNA helicase DDX20, also known as DEAD-box helicase 20 and gem-associated protein 3 (GEMIN3), is an enzyme that in humans is encoded by the DDX20 gene.[1][2]

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neuron (SMN) complex.[2] This DEAD box protein is one of the gem-associated proteins (GEMIN3) that interacts directly with SMN.[3] SMN is the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.[2]

Biological Implication

Previous research has revealed that DDX20 may act as a tumor suppressor in hepatocellular carcinoma and as a tumor promoter in breast cancer. DDX20 deficiency enhances NF-κB activity by impairing the NF-κB-suppressive action of microRNAs, and suggest that dysregulation of the microRNA machinery components may also be involved in pathogenesis in various human diseases.[4] Such as miRNA-140 which acts as a liver tumor suppressor, and due to a deficiency of DDX20, miRNA-140 function gets impair, this subsequent functional impairment of miRNAs could lead to hepatocarcinogenesis.Similarly,[5] DDX20 may promote the progression of Prostate cancer (PCa) through the NF-κB pathway.[6] In a clinical based study it has been observed that positive DP103/NF-κB feedback loop promotes constitutive NF-κB activation in invasive breast cancers and activation of this pathway is linked to cancer progression and the acquisition of chemotherapy resistance. It makes DP103 has potential as a therapeutic target for breast cancer treatment.[7]

Interactions

DDX20 has been shown to interact with:

References

  1. ↑ Grundhoff AT, Kremmer E, Türeci O, Glieden A, Gindorf C, Atz J, Mueller-Lantzsch N, Schubach WH, Grässer FA (July 1999). "Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C". J Biol Chem. 274 (27): 19136–44. doi:10.1074/jbc.274.27.19136. PMID 10383418.
  2. ↑ 2.0 2.1 2.2 "Entrez Gene: DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20".
  3. ↑ Seng, CO; Magee, C; Young, PJ; Lorson, CL; Allen, JP (15 April 2015). "The SMN structure reveals its crucial role in snRNP assembly". Human Molecular Genetics. 24 (8): 2138–46. doi:10.1093/hmg/ddu734. PMID 25561692.
  4. ↑ Takata, Akemi; Otsuka, Motoyuki; Yoshikawa, Takeshi; Kishikawa, Takahiro; Kudo, Yotaro; Goto, Tadashi; Yoshida, Haruhiko; Koike, Kazuhiko (2012-04-13). "A miRNA machinery component DDX20 controls NF-κB via microRNA-140 function". Biochemical and Biophysical Research Communications. 420 (3): 564–569. doi:10.1016/j.bbrc.2012.03.034. ISSN 1090-2104. PMID 22445758.
  5. ↑ Takata, Akemi; Otsuka, Motoyuki; Yoshikawa, Takeshi; Kishikawa, Takahiro; Hikiba, Yohko; Obi, Shuntaro; Goto, Tadashi; Kang, Young Jun; Maeda, Shin (January 2013). "MicroRNA-140 acts as a liver tumor suppressor by controlling NF-κB activity by directly targeting DNA methyltransferase 1 (Dnmt1) expression". Hepatology. 57 (1): 162–170. doi:10.1002/hep.26011. ISSN 1527-3350. PMC 3521841. PMID 22898998.
  6. ↑ Chen, Weiguo; Zhou, Peng; Li, Xiaowei (June 2016). "High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway". International Journal of Molecular Medicine. 37 (6): 1551–1557. doi:10.3892/ijmm.2016.2575. ISSN 1791-244X. PMC 4866965. PMID 27121695.
  7. ↑ Shin, Eun Myoung; Hay, Hui Sin; Lee, Moon Hee; Goh, Jen Nee; Tan, Tuan Zea; Sen, Yin Ping; Lim, See Wee; Yousef, Einas M.; Ong, Hooi Tin (September 2014). "DEAD-box helicase DP103 defines metastatic potential of human breast cancers". The Journal of Clinical Investigation. 124 (9): 3807–3824. doi:10.1172/JCI73451. ISSN 1558-8238. PMC 4151228. PMID 25083991.
  8. ↑ 8.0 8.1 8.2 8.3 8.4 Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rappsilber J, Mann M, Dreyfuss G (March 2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC 155365. PMID 11914277.
  9. ↑ Nelson PT, Hatzigeorgiou AG, Mourelatos Z (March 2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA. 10 (3): 387–94. doi:10.1261/rna.5181104. PMC 1370934. PMID 14970384.
  10. ↑ 10.0 10.1 Carnegie GK, Sleeman JE, Morrice N, Hastie CJ, Peggie MW, Philp A, Lamond AI, Cohen PT (May 2003). "Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs". J. Cell Sci. 116 (Pt 10): 1905–13. doi:10.1242/jcs.00409. PMID 12668731.
  11. ↑ Charroux B, Pellizzoni L, Perkinson RA, Yong J, Shevchenko A, Mann M, Dreyfuss G (March 2000). "Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli". J. Cell Biol. 148 (6): 1177–86. doi:10.1083/jcb.148.6.1177. PMC 2174312. PMID 10725331.
  12. ↑ Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G (February 2002). "Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins". J. Biol. Chem. 277 (7): 5631–6. doi:10.1074/jbc.M109448200. PMID 11714716.
  13. ↑ 13.0 13.1 13.2 13.3 13.4 13.5 13.6 13.7 13.8 13.9 Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G (December 1999). "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems". J. Cell Biol. 147 (6): 1181–94. doi:10.1083/jcb.147.6.1181. PMC 2168095. PMID 10601333.
  14. ↑ Meister G, Bühler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U (August 2000). "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins". Hum. Mol. Genet. 9 (13): 1977–86. doi:10.1093/hmg/9.13.1977. PMID 10942426.

Further reading

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