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{{Infobox_gene}}
{{Infobox protein family
| Symbol = INCENP_N
| Name = Chromosome passenger complex (CPC) protein INCENP N terminal
| image =
| width =
| caption = crystal structure of a survivin-borealin-incenp core complex
| Pfam = PF12178
| Pfam_clan = 
| InterPro = IPR022006
| SMART =
| PROSITE =
| MEROPS =
| SCOP =
| TCDB =
| OPM family =
| OPM protein =
| CAZy =
| CDD =
}}
{{Infobox protein family
| Symbol = INCENP_ARK-bind
| Name = Inner centromere protein, ARK binding region
| image =
| width =
| caption = crystal structure of aurora b kinase in complex with a aminothiazole inhibitor
| Pfam = PF03941
| Pfam_clan = 
| InterPro = IPR005635
| SMART =
| PROSITE =
| MEROPS =
| SCOP =
| TCDB =
| OPM family =
| OPM protein =
| CAZy =
| CDD =
}}
'''Inner centromere protein''' is a [[protein]] that in humans is encoded by the ''INCENP'' [[gene]].<ref name="pmid1860899">{{cite journal |vauthors=Earnshaw WC, Cooke CA | title = Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation | journal = J Cell Sci | volume = 98 | issue =  4| pages = 443–61 |date=Sep 1991 | pmid = 1860899 | pmc =  | doi =  }}</ref><ref name="pmid11453556">{{cite journal |vauthors=Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC | title = Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells | journal = Chromosoma | volume = 110 | issue = 2 | pages = 65–74 |date=Jul 2001 | pmid = 11453556 | pmc =  | doi =10.1007/s004120100130  }}</ref><ref name="entrez">{{Cite web| title = Entrez Gene: INCENP inner centromere protein antigens 135/155kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3619| accessdate = }}</ref>
In mammalian cells, two broad groups of [[centromere]]-interacting proteins have been described: constitutively binding [[centromere]] proteins and 'passenger' (or transiently interacting) proteins.<ref name="isbn0-19-857780-X">{{Cite book| author = Choo, K. H. Andy | title = The centromere | edition = | language = | publisher = Oxford University Press | location = Oxford [Oxfordshire] | year = 1997 | origyear = | pages = | quote = | isbn = 0-19-857780-X | oclc = | doi = | url = | accessdate = }}</ref> The constitutive proteins include [[CENPA]] (centromere protein A), [[centromere protein B|CENPB]], [[CENPC1]], and [[RCC1|CENPD]].
The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the [[cell cycle]].<ref name="pmid8088460">{{cite journal |vauthors=Earnshaw WC, Mackay AM | title = Role of nonhistone proteins in the chromosomal events of mitosis | journal = FASEB J. | volume = 8 | issue = 12 | pages = 947–56 |date=September 1994 | pmid = 8088460 | doi = | url = | issn = }}</ref> These include [[centromere protein E|CENPE]]; [[KIF2C|MCAK]]; [[KIF22|KID]]; cytoplasmic dynein (e.g., [[DYNC1H1]]); CliPs (e.g. [[CLIP1]]); and CENPF/mitosin ([[CENPF]]). The inner centromere proteins (INCENPs),<ref name="pmid1860899" /> the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of [[mitosis]] but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During [[telophase]], the proteins are located within the [[midbody (cell biology)|midbody]] in the intercellular bridge, where they are discarded after [[cytokinesis]].<ref name="entrez"/><ref name="pmid10369859">{{cite journal |vauthors=Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH | title = Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice | journal = Hum. Mol. Genet. | volume = 8 | issue = 7 | pages = 1145–55 |date=July 1999 | pmid= 10369859 | doi = 10.1093/hmg/8.7.1145| url = | issn = }}</ref>
INCENP is a regulatory [[protein]] in the [[chromosome]] passenger [[Protein complex|complex]]. It is involved in regulation of the [[catalytic]] protein [[Aurora B]]. It performs this function in association with two other [[protein]]s - [[Survivin]] and [[CDCA8|Borealin]]. These [[proteins]] form a tight three-helical bundle. The [[N-terminal]] [[protein domain|domain]] of INCENP is the domain involved in formation of this three-helical bundle.<ref name="pmid17956729">{{Cite journal
| last1 = Jeyaprakash | first1 = A. A.
| last2 = Klein | first2 = U. R.
| last3 = Lindner | first3 = D.
| last4 = Ebert | first4 = J.
| last5 = Nigg | first5 = E. A.
| last6 = Conti | first6 = E.
| doi = 10.1016/j.cell.2007.07.045
| title = Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together
| journal = Cell
| volume = 131
| issue = 2
| pages = 271–285
| year = 2007
| pmid = 17956729
}}</ref>
==Interactions==
INCENP has been shown to [[Protein-protein interaction|interact]] with [[H2AFZ]],<ref name="pmid12660166">{{cite journal |vauthors=Rangasamy D, Berven L, Ridgway P, Tremethick DJ | title = Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development | journal = EMBO J. | volume = 22 | issue = 7 | pages = 1599–607 |date=April 2003 | pmid = 12660166 | pmc = 152904 | doi = 10.1093/emboj/cdg160 | url = | issn = }}</ref> [[Survivin]]<ref name="pmid11516652">{{cite journal |vauthors=Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC | title = INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis | journal = Curr. Biol. | volume = 11 | issue = 11 | pages = 886–90 |date=June 2001 | pmid = 11516652 | doi = 10.1016/S0960-9822(01)00238-X| url = | issn = }}</ref> and [[CDCA8]].<ref name="pmid15249581">{{cite journal |vauthors=Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC | title = Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle | journal = J. Cell Biol. | volume = 166 | issue = 2 | pages = 179–91 |date=July 2004 | pmid = 15249581 | pmc = 2172304 | doi = 10.1083/jcb.200404001 | url = | issn = }}</ref> The ARK binding region has been found to be necessary and sufficient for binding to aurora-related [[kinase]]. This interaction has been implicated in the coordination of [[chromosome segregation]] with [[cell cycle|cell division]] in [[yeast]].<ref name="pmid11950927">{{cite journal |vauthors=Leverson JD, Huang HK, Forsburg SL, Hunter T | title = The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis | journal = Mol. Biol. Cell | volume = 13 | issue = 4 | pages = 1132–43 |date=April 2002 | pmid = 11950927 | pmc = 102257 | doi = 10.1091/mbc.01-07-0330 | url = }}</ref>
==References==
{{Reflist}}
==Further reading==
{{Refbegin| 2}}
{{PBB_Further_reading
| citations =
*{{Cite journal  |vauthors=Ainsztein AM, Kandels-Lewis SE, Mackay AM, Earnshaw WC |title=INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1. |journal=J. Cell Biol. |volume=143 |issue= 7 |pages= 1763–74 |year= 1999 |pmid= 9864353 |doi=10.1083/jcb.143.7.1763  | pmc=2175214  }}
*{{Cite journal  |vauthors=Martineau-Thuillier S, Andreassen PR, Margolis RL |title=Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis. |journal=Chromosoma |volume=107 |issue= 6-7 |pages= 461–70 |year= 1999 |pmid= 9914378 |doi=10.1007/s004120050330  }}
*{{Cite journal  |vauthors=Dias Neto E, Correa RG, Verjovski-Almeida S, etal |title=Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=97 |issue= 7 |pages= 3491–6 |year= 2000 |pmid= 10737800 |doi=10.1073/pnas.97.7.3491  | pmc=16267  }}
*{{Cite journal  |vauthors=Wheatley SP, Kandels-Lewis SE, Adams RR, etal |title=INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow. |journal=Exp. Cell Res. |volume=262 |issue= 2 |pages= 122–7 |year= 2001 |pmid= 11139336 |doi= 10.1006/excr.2000.5088 }}
*{{Cite journal  |vauthors=Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC |title=INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis. |journal=Curr. Biol. |volume=11 |issue= 11 |pages= 886–90 |year= 2001 |pmid= 11516652 |doi=10.1016/S0960-9822(01)00238-X  }}
*{{Cite journal  |vauthors=Strausberg RL, Feingold EA, Grouse LH, etal |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899  | pmc=139241 }}
*{{Cite journal  |vauthors=Parra MT, Viera A, Gómez R, etal |title=Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis. |journal=J. Cell Sci. |volume=116 |issue= Pt 6 |pages= 961–74 |year= 2003 |pmid= 12584241 |doi=10.1242/jcs.00330  }}
*{{Cite journal  |vauthors=Rangasamy D, Berven L, Ridgway P, Tremethick DJ |title=Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development. |journal=EMBO J. |volume=22 |issue= 7 |pages= 1599–607 |year= 2003 |pmid= 12660166 |doi= 10.1093/emboj/cdg160  | pmc=152904 }}
*{{Cite journal  |vauthors=Honda R, Körner R, Nigg EA |title=Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis. |journal=Mol. Biol. Cell |volume=14 |issue= 8 |pages= 3325–41 |year= 2004 |pmid= 12925766 |doi= 10.1091/mbc.E02-11-0769  | pmc=181570 }}
*{{Cite journal  |vauthors=Wheatley SP, Henzing AJ, Dodson H, etal |title=Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo. |journal=J. Biol. Chem. |volume=279 |issue= 7 |pages= 5655–60 |year= 2004 |pmid= 14610074 |doi= 10.1074/jbc.M311299200 }}
*{{Cite journal  |vauthors=Ota T, Suzuki Y, Nishikawa T, etal |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
*{{Cite journal  |vauthors=Gassmann R, Carvalho A, Henzing AJ, etal |title=Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. |journal=J. Cell Biol. |volume=166 |issue= 2 |pages= 179–91 |year= 2004 |pmid= 15249581 |doi= 10.1083/jcb.200404001  | pmc=2172304 }}
*{{Cite journal  |vauthors=Li X, Sakashita G, Matsuzaki H, etal |title=Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C. |journal=J. Biol. Chem. |volume=279 |issue= 45 |pages= 47201–11 |year= 2004 |pmid= 15316025 |doi= 10.1074/jbc.M403029200 }}
*{{Cite journal  |vauthors=Gerhard DS, Wagner L, Feingold EA, etal |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504  | pmc=528928 }}
*{{Cite journal  |vauthors=Zhu C, Bossy-Wetzel E, Jiang W |title=Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells. |journal=Biochem. J. |volume=389 |issue= Pt 2 |pages= 373–81 |year= 2005 |pmid= 15796717 |doi= 10.1042/BJ20050097  | pmc=1175114 }}
*{{Cite journal  |vauthors=Chen HL, Tang CJ, Chen CY, Tang TK |title=Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy. |journal=J. Biomed. Sci. |volume=12 |issue= 2 |pages= 297–310 |year= 2005 |pmid= 15917996 |doi= 10.1007/s11373-005-0980-0 }}
*{{Cite journal  |vauthors=Vader G, Kauw JJ, Medema RH, Lens SM |title=Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody. |journal=EMBO Rep. |volume=7 |issue= 1 |pages= 85–92 |year= 2006 |pmid= 16239925 |doi= 10.1038/sj.embor.7400562  | pmc=1369225 }}
*{{Cite journal  |vauthors=Goto H, Kiyono T, Tomono Y, etal |title=Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. |journal=Nat. Cell Biol. |volume=8 |issue= 2 |pages= 180–7 |year= 2006 |pmid= 16378098 |doi= 10.1038/ncb1350 }}
}}
{{Refend}}
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<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
{{InterPro content|IPR005635}}
{{GNF_Protein_box
| image = 
| image_source = 
| PDB =
| Name = Inner centromere protein antigens 135/155kDa
| HGNCid = 6058
| Symbol = INCENP
| AltSymbols =; FLJ31633; MGC111393
| OMIM = 604411
| ECnumber = 
| Homologene = 9624
| MGIid = 1313288
| GeneAtlas_image1 = PBB_GE_INCENP_219769_at_tn.png
| Function = {{GNF_GO|id=GO:0005515 |text = protein binding}}
| Component = {{GNF_GO|id=GO:0000801 |text = central element}} {{GNF_GO|id=GO:0005634 |text = nucleus}} {{GNF_GO|id=GO:0005721 |text = centric heterochromatin}} {{GNF_GO|id=GO:0005819 |text = spindle}} {{GNF_GO|id=GO:0005874 |text = microtubule}} {{GNF_GO|id=GO:0030496 |text = midbody}} {{GNF_GO|id=GO:0043234 |text = protein complex}}
| Process = {{GNF_GO|id=GO:0000910 |text = cytokinesis}} {{GNF_GO|id=GO:0007049 |text = cell cycle}} {{GNF_GO|id=GO:0007059 |text = chromosome segregation}} {{GNF_GO|id=GO:0007067 |text = mitosis}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 3619
    | Hs_Ensembl = ENSG00000149503
    | Hs_RefseqProtein = NP_001035784
    | Hs_RefseqmRNA = NM_001040694
    | Hs_GenLoc_db = 
    | Hs_GenLoc_chr = 11
    | Hs_GenLoc_start = 61648021
    | Hs_GenLoc_end = 61676372
    | Hs_Uniprot = Q9NQS7
    | Mm_EntrezGene = 16319
    | Mm_Ensembl = ENSMUSG00000024660
    | Mm_RefseqmRNA = NM_016692
    | Mm_RefseqProtein = NP_057901
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 19
    | Mm_GenLoc_start = 9939391
    | Mm_GenLoc_end = 9966514
    | Mm_Uniprot = Q9WU62
  }}
}}
'''Inner centromere protein antigens 135/155kDa''', also known as '''INCENP''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: INCENP inner centromere protein antigens 135/155kDa| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3619| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
{{InterPro content|IPR022006}}
{{PBB_Summary
| section_title =
| summary_text = In mammalian cells, 2 broad groups of [[centromere]]-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994). These include CENPE (MIM 117143); [[M Type Kinesins|MCAK]] (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999).[supplied by OMIM]<ref name="entrez">{{cite web | title = Entrez Gene: INCENP inner centromere protein antigens 135/155kDa| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3619| accessdate = }}</ref>
}}
 
==References==
{{reflist|2}}
==Further reading==
{{refbegin | 2}}
{{PBB_Further_reading
| citations =
*{{cite journal  | author=Earnshaw WC, Cooke CA |title=Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation. |journal=J. Cell. Sci. |volume=98 ( Pt 4) |issue=  |pages= 443-61 |year= 1991 |pmid= 1860899 |doi=  }}
*{{cite journal  | author=Ainsztein AM, Kandels-Lewis SE, Mackay AM, Earnshaw WC |title=INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1. |journal=J. Cell Biol. |volume=143 |issue= 7 |pages= 1763-74 |year= 1999 |pmid= 9864353 |doi=  }}
*{{cite journal  | author=Martineau-Thuillier S, Andreassen PR, Margolis RL |title=Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis. |journal=Chromosoma |volume=107 |issue= 6-7 |pages= 461-70 |year= 1999 |pmid= 9914378 |doi=  }}
*{{cite journal  | author=Dias Neto E, Correa RG, Verjovski-Almeida S, ''et al.'' |title=Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=97 |issue= 7 |pages= 3491-6 |year= 2000 |pmid= 10737800 |doi=  }}
*{{cite journal  | author=Wheatley SP, Kandels-Lewis SE, Adams RR, ''et al.'' |title=INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow. |journal=Exp. Cell Res. |volume=262 |issue= 2 |pages= 122-7 |year= 2001 |pmid= 11139336 |doi= 10.1006/excr.2000.5088 }}
*{{cite journal  | author=Adams RR, Eckley DM, Vagnarelli P, ''et al.'' |title=Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells. |journal=Chromosoma |volume=110 |issue= 2 |pages= 65-74 |year= 2001 |pmid= 11453556 |doi=  }}
*{{cite journal  | author=Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC |title=INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis. |journal=Curr. Biol. |volume=11 |issue= 11 |pages= 886-90 |year= 2001 |pmid= 11516652 |doi=  }}
*{{cite journal  | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
*{{cite journal  | author=Parra MT, Viera A, Gómez R, ''et al.'' |title=Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis. |journal=J. Cell. Sci. |volume=116 |issue= Pt 6 |pages= 961-74 |year= 2003 |pmid= 12584241 |doi=  }}
*{{cite journal  | author=Rangasamy D, Berven L, Ridgway P, Tremethick DJ |title=Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development. |journal=EMBO J. |volume=22 |issue= 7 |pages= 1599-607 |year= 2003 |pmid= 12660166 |doi= 10.1093/emboj/cdg160 }}
*{{cite journal  | author=Honda R, Körner R, Nigg EA |title=Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis. |journal=Mol. Biol. Cell |volume=14 |issue= 8 |pages= 3325-41 |year= 2004 |pmid= 12925766 |doi= 10.1091/mbc.E02-11-0769 }}
*{{cite journal  | author=Wheatley SP, Henzing AJ, Dodson H, ''et al.'' |title=Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo. |journal=J. Biol. Chem. |volume=279 |issue= 7 |pages= 5655-60 |year= 2004 |pmid= 14610074 |doi= 10.1074/jbc.M311299200 }}
*{{cite journal  | author=Ota T, Suzuki Y, Nishikawa T, ''et al.'' |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40-5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
*{{cite journal  | author=Gassmann R, Carvalho A, Henzing AJ, ''et al.'' |title=Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. |journal=J. Cell Biol. |volume=166 |issue= 2 |pages= 179-91 |year= 2004 |pmid= 15249581 |doi= 10.1083/jcb.200404001 }}
*{{cite journal  | author=Li X, Sakashita G, Matsuzaki H, ''et al.'' |title=Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C. |journal=J. Biol. Chem. |volume=279 |issue= 45 |pages= 47201-11 |year= 2004 |pmid= 15316025 |doi= 10.1074/jbc.M403029200 }}
*{{cite journal  | author=Gerhard DS, Wagner L, Feingold EA, ''et al.'' |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121-7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 }}
*{{cite journal  | author=Zhu C, Bossy-Wetzel E, Jiang W |title=Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells. |journal=Biochem. J. |volume=389 |issue= Pt 2 |pages= 373-81 |year= 2005 |pmid= 15796717 |doi= 10.1042/BJ20050097 }}
*{{cite journal  | author=Chen HL, Tang CJ, Chen CY, Tang TK |title=Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy. |journal=J. Biomed. Sci. |volume=12 |issue= 2 |pages= 297-310 |year= 2005 |pmid= 15917996 |doi= 10.1007/s11373-005-0980-0 }}
*{{cite journal  | author=Vader G, Kauw JJ, Medema RH, Lens SM |title=Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody. |journal=EMBO Rep. |volume=7 |issue= 1 |pages= 85-92 |year= 2006 |pmid= 16239925 |doi= 10.1038/sj.embor.7400562 }}
*{{cite journal  | author=Goto H, Kiyono T, Tomono Y, ''et al.'' |title=Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. |journal=Nat. Cell Biol. |volume=8 |issue= 2 |pages= 180-7 |year= 2006 |pmid= 16378098 |doi= 10.1038/ncb1350 }}
}}
{{refend}}


{{protein-stub}}
[[Category:Protein families]]
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Latest revision as of 20:06, 8 November 2017

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Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

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Ensembl

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UniProt

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RefSeq (mRNA)

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RefSeq (protein)

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Location (UCSC)n/an/a
PubMed searchn/an/a
Wikidata
View/Edit Human
Chromosome passenger complex (CPC) protein INCENP N terminal
Identifiers
SymbolINCENP_N
PfamPF12178
InterProIPR022006
Inner centromere protein, ARK binding region
Identifiers
SymbolINCENP_ARK-bind
PfamPF03941
InterProIPR005635

Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[1][2][3]

In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[4] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.

The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[5] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[1] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.[3][6]

INCENP is a regulatory protein in the chromosome passenger complex. It is involved in regulation of the catalytic protein Aurora B. It performs this function in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle.[7]

Interactions

INCENP has been shown to interact with H2AFZ,[8] Survivin[9] and CDCA8.[10] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related kinase. This interaction has been implicated in the coordination of chromosome segregation with cell division in yeast.[11]

References

  1. ↑ 1.0 1.1 Earnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci. 98 (4): 443–61. PMID 1860899.
  2. ↑ Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma. 110 (2): 65–74. doi:10.1007/s004120100130. PMID 11453556.
  3. ↑ 3.0 3.1 "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa".
  4. ↑ Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire]: Oxford University Press. ISBN 0-19-857780-X.
  5. ↑ Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB J. 8 (12): 947–56. PMID 8088460.
  6. ↑ Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Hum. Mol. Genet. 8 (7): 1145–55. doi:10.1093/hmg/8.7.1145. PMID 10369859.
  7. ↑ Jeyaprakash, A. A.; Klein, U. R.; Lindner, D.; Ebert, J.; Nigg, E. A.; Conti, E. (2007). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together". Cell. 131 (2): 271–285. doi:10.1016/j.cell.2007.07.045. PMID 17956729.
  8. ↑ Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development". EMBO J. 22 (7): 1599–607. doi:10.1093/emboj/cdg160. PMC 152904. PMID 12660166.
  9. ↑ Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Curr. Biol. 11 (11): 886–90. doi:10.1016/S0960-9822(01)00238-X. PMID 11516652.
  10. ↑ Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. doi:10.1083/jcb.200404001. PMC 2172304. PMID 15249581.
  11. ↑ Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002). "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis". Mol. Biol. Cell. 13 (4): 1132–43. doi:10.1091/mbc.01-07-0330. PMC 102257. PMID 11950927.

Further reading


This article incorporates text from the public domain Pfam and InterPro: IPR005635
This article incorporates text from the public domain Pfam and InterPro: IPR022006
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