TIMM50: Difference between revisions

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'''Mitochondrial import inner membrane translocase subunit TIM50''' is an [[enzyme]] that in humans is encoded by the ''TIMM50'' [[gene]].<ref name="pmid12437925">{{cite journal | vauthors = Yamamoto H, Esaki M, Kanamori T, Tamura Y, Nishikawa S, Endo T | title = Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes | journal = Cell | volume = 111 | issue = 4 | pages = 519–28 |date=Nov 2002 | pmid = 12437925 | pmc =  | doi =10.1016/S0092-8674(02)01053-X }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: TIMM50 translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=92609| accessdate = }}</ref>
'''Mitochondrial import inner membrane translocase subunit TIM50''' is an [[enzyme]] that in humans is encoded by the ''TIMM50'' [[gene]]. Tim50 is a subunit of the Tim23 translocase complex in the inner mitochondrial membrane. <ref name="pmid12437925">{{cite journal | vauthors = Yamamoto H, Esaki M, Kanamori T, Tamura Y, Nishikawa S, Endo T | title = TIM50 is a subunit of the Tim23 complex which links protein translocation across the outer and inner mitochondrial membranes | journal = Cell | volume = 111 | issue = 4 | pages = 519–28 | date = November 2002 | pmid = 12437925 | pmc =  | doi = 10.1016/S0092-8674(02)01053-X }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: TIMM50 translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=92609 }}</ref> Mutations in ''TIMM50'' can lead to [[epilepsy]], severe intellectual disability, and [[3-methylglutaconic aciduria]].<ref name="Shahrour_2017">{{cite journal | vauthors = Shahrour MA, Staretz-Chacham O, Dayan D, Stephen J, Weech A, Damseh N, Pri Chen H, Edvardson S, Mazaheri S, Saada A, Hershkovitz E, Shaag A, Huizing M, Abu-Libdeh B, Gahl WA, Azem A, Anikster Y, Vilboux T, Elpeleg O, Malicdan MC | display-authors = 6 | title = Mitochondrial epileptic encephalopathy, 3-methylglutaconic aciduria and variable complex V deficiency associated with TIMM50 mutations | journal = Clinical Genetics | volume = 91 | issue = 5 | pages = 690–696 | date = May 2017 | pmid = 27573165 | doi = 10.1111/cge.12855 }}</ref> ''TIMM50'' expression is increased in breast cancer cells<ref name="Gao_2016">{{cite journal | vauthors = Gao SP, Sun HF, Jiang HL, Li LD, Hu X, Xu XE, Jin W | title = Loss of TIM50 suppresses proliferation and induces apoptosis in breast cancer | journal = Tumor Biology | volume = 37 | issue = 1 | pages = 1279-87 | date = January 2016 | pmid = 26289846 | doi = 10.1007/s13277-015-3878-0 }}</ref> and decreased in hypertrophic hearts.<ref name="Tang_2017">{{cite journal | vauthors = Tang K, Zhao Y, Li H, Zhu M, Li W, Liu W, Zhu G, Xu D, Peng W, Xu YW | display-authors = 6 | title = Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy | journal = Journal of the American Heart Association | volume = 6 | issue = 4 | pages = e004346 | date = April 2017 | pmid = 28432072 | doi = 10.1161/JAHA.116.004346 }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot. See Template:PBB_Controls to Stop updates. -->
== Structure ==
{{PBB_Summary
The ''TIMM50'' gene is located on the q arm of [[chromosome 19]] in position 13.2 and spans 13,373 base pairs. <ref name = "entrez"/> The gene produces a 39.6 kDa protein composed of 353 [[amino acids]].<ref name=COPaKB> {{cite journal | vauthors = Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, Deng N, Kim AK, Choi JH, Zelaya I, Liem D, Meyer D, Odeberg J, Fang C, Lu HJ, Xu T, Weiss J, Duan H, Uhlen M, Yates JR, Apweiler R, Ge J, Hermjakob H, Ping P | display-authors = 6 | title = Integration of cardiac proteome biology and medicine by a specialized knowledgebase | journal = Circulation Research | volume = 113 | issue = 9 | pages = 1043–53 | date = October 2013 | pmid = 23965338 | pmc = 4076475 | doi = 10.1161/CIRCRESAHA.113.301151 }}</ref><ref name="url_COPaKB">{{cite web | url = https://amino.heartproteome.org/web/protein/Q3ZCQ8 | work = Cardiac Organellar Protein Atlas Knowledgebase (COPaKB) | title = Mitochondrial import inner membrane translocase subunit TIM50 }}</ref> This gene encodes a subunit of the [[Translocase_of_the_inner_membrane#The_TIM23_complex |TIM23]] inner mitochondrial membrane translocase complex, which mediates the translocation of transit peptide-containing proteins across the [[Mitochondrion#Inner_membrane |mitochondrial inner membrane]]. <ref name = "entrez"/>
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==References==
== Function ==
The Tim50 protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and [[Mitochondrion#Matrix |matrix]]. This gene in human cells results in the release of [[Cytochrome c oxidase |cytochrome c]] and [[apoptosis]].<ref name = "entrez"/> This protein plays a role in maintaining the membrane permeability barrier. The [[Mitochondrion#Intermembrane_space| intermembrane space]] domain of Tim50 induces the translocation pore of the TIM23 channel to close. <ref name="pmid12437925"/><ref name="entrez"/>
 
== Clinical significance ==
Missense mutations in ''TIMM50'' often result in epilepsy or epileptic [[encephalopathy]], severe intellectual disability, variable mitochondrial complex V deficiency, and 3-methylglutaconic aciduria, which is a key biomarker for mitochondrial membrane defects and mitochondrial dysfunction. Inheritance of ''TIMM50'' is [[Genetic_disorder#Autosomal_recessive |autosomal recessive]]. <ref name="Shahrour_2017" /> Expression of the TIMM50 gene is increased in [[breast cancer]] cells. In such cells, overexpression of the Tim50 protein is linked to lack of cellular apoptosis and increased rates of proliferation.<ref name="Gao_2016" /> Decreased ''TIMM50'' expression in heart cells can lead to cardiac [[Ventricular hypertrophy |hypertrophy]].<ref name="Tang_2017" />
 
Two patients, male and female siblings born to consanguineous Bedouin parents were presented, displaying involuntary abnormal movements, failure to thrive, [[hypsarrhythmia]], bilateral optic atrophy, 3-methylglutaconic aciduria, and slightly elevated plasma [[Lactic_acid#Biology |lactate]] levels. Both began walking independently at only 3 years and initially received favorably [[Adrenocorticotropic hormone |ACTH]] therapy until switching to a treatment of [[Valproate]] with either [[Vigabatrin |Sabril]] or [[Topiramate |Topamax]], which resulted in seizures completely disappearing. Two more patients, male and female siblings born to first-cousin parents of Muslim origin were also presented, displaying myoclonic and tonic seizures, abnormal [[Electroencephalography |EEG]], brain atrophy, delayed [[Psychomotor learning |psychomotor development]] and 3-methylglutaconic aciduria. Treatment of [[Lamotrigine |Lamictal]] combined with Valproate was effective in controlling the seizures.<ref name="Shahrour_2017" />
 
== Interactions ==
Within the TIM23 complex, the Tim50 subunit directly interacts with ''TIMM23''. The TIM23 complex interacts with the [[TIMM44 |''TIMM44'']] component of the PAM complex and with ''DNAJC15''. An isoform of Tim50 interacts with [[Coilin |''COIL'']] and [[snRNP |snRNPs]].<ref>{{Cite web|url=https://www.uniprot.org/uniprot/Q3ZCQ8|title=TIMM50 - Mitochondrial import inner membrane translocase subunit TIM50 precursor - Homo sapiens (Human) - TIMM50 gene & protein|website=www.uniprot.org|language=en|access-date=2018-07-25}}</ref>
 
== Reference ==
{{reflist}}
{{reflist}}


==Further reading==
== Further reading ==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
* {{cite journal | vauthors = Xu H, Somers ZB, Robinson ML, Hebert MD | title = Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis | journal = BMC Cell Biology | volume = 6 | issue = 1 | pages = 29 | date = July 2005 | pmid = 16008839 | pmc = 1177934 | doi = 10.1186/1471-2121-6-29 }}
| citations =
* {{cite journal | vauthors = Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES | title = Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death | journal = The Journal of Biological Chemistry | volume = 279 | issue = 23 | pages = 24813–25 | date = June 2004 | pmid = 15044455 | doi = 10.1074/jbc.M402049200 }}
*{{cite journal   |vauthors=Ewing RM, Chu P, Elisma F, etal |title=Large-scale mapping of human protein-protein interactions by mass spectrometry. |journal=Mol. Syst. Biol. |volume=3 |issue=  1|pages= 89 |year= 2007 |pmid= 17353931 |doi= 10.1038/msb4100134  | pmc=1847948 }}
* {{cite journal | vauthors = Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G | display-authors = 6 | title = A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway | journal = Nature Cell Biology | volume = 6 | issue = 2 | pages = 97–105 | date = February 2004 | pmid = 14743216 | doi = 10.1038/ncb1086 }}
*{{cite journal  | vauthors=Xu H, Somers ZB, Robinson ML, Hebert MD |title=Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis. |journal=BMC Cell Biol. |volume=6 |pages= 29 |year= 2006 |pmid= 16008839 |doi= 10.1186/1471-2121-6-29 | pmc=1177934 }}
* {{cite journal | vauthors = Yuryev A, Wennogle LP | title = Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis | journal = Genomics | volume = 81 | issue = 2 | pages = 112–25 | date = February 2003 | pmid = 12620389 | doi = 10.1016/S0888-7543(02)00008-3 }}
*{{cite journal   |vauthors=Gerhard DS, Wagner L, Feingold EA, etal |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504  | pmc=528928 }}
*{{cite journal  |vauthors=Guo Y, Cheong N, Zhang Z, etal |title=Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. |journal=J. Biol. Chem. |volume=279 |issue= 23 |pages= 24813–25 |year= 2004 |pmid= 15044455 |doi= 10.1074/jbc.M402049200 }}
*{{cite journal   |vauthors=Bouwmeester T, Bauch A, Ruffner H, etal |title=A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. |journal=Nat. Cell Biol. |volume=6 |issue= 2 |pages= 97–105 |year= 2004 |pmid= 14743216 |doi= 10.1038/ncb1086 }}
*{{cite journal   |vauthors=Ota T, Suzuki Y, Nishikawa T, etal |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40–5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
*{{cite journal  | vauthors=Yuryev A, Wennogle LP |title=Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis. |journal=Genomics |volume=81 |issue= 2 |pages= 112–25 |year= 2003 |pmid= 12620389 |doi=10.1016/S0888-7543(02)00008-3 }}
*{{cite journal  |vauthors=Strausberg RL, Feingold EA, Grouse LH, etal |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899  | pmc=139241 }}
}}
{{refend}}
{{refend}}


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Latest revision as of 21:11, 25 July 2018

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Identifiers
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External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

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UniProt

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Mitochondrial import inner membrane translocase subunit TIM50 is an enzyme that in humans is encoded by the TIMM50 gene. Tim50 is a subunit of the Tim23 translocase complex in the inner mitochondrial membrane. [1][2] Mutations in TIMM50 can lead to epilepsy, severe intellectual disability, and 3-methylglutaconic aciduria.[3] TIMM50 expression is increased in breast cancer cells[4] and decreased in hypertrophic hearts.[5]

Structure

The TIMM50 gene is located on the q arm of chromosome 19 in position 13.2 and spans 13,373 base pairs. [2] The gene produces a 39.6 kDa protein composed of 353 amino acids.[6][7] This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex, which mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. [2]

Function

The Tim50 protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This gene in human cells results in the release of cytochrome c and apoptosis.[2] This protein plays a role in maintaining the membrane permeability barrier. The intermembrane space domain of Tim50 induces the translocation pore of the TIM23 channel to close. [1][2]

Clinical significance

Missense mutations in TIMM50 often result in epilepsy or epileptic encephalopathy, severe intellectual disability, variable mitochondrial complex V deficiency, and 3-methylglutaconic aciduria, which is a key biomarker for mitochondrial membrane defects and mitochondrial dysfunction. Inheritance of TIMM50 is autosomal recessive. [3] Expression of the TIMM50 gene is increased in breast cancer cells. In such cells, overexpression of the Tim50 protein is linked to lack of cellular apoptosis and increased rates of proliferation.[4] Decreased TIMM50 expression in heart cells can lead to cardiac hypertrophy.[5]

Two patients, male and female siblings born to consanguineous Bedouin parents were presented, displaying involuntary abnormal movements, failure to thrive, hypsarrhythmia, bilateral optic atrophy, 3-methylglutaconic aciduria, and slightly elevated plasma lactate levels. Both began walking independently at only 3 years and initially received favorably ACTH therapy until switching to a treatment of Valproate with either Sabril or Topamax, which resulted in seizures completely disappearing. Two more patients, male and female siblings born to first-cousin parents of Muslim origin were also presented, displaying myoclonic and tonic seizures, abnormal EEG, brain atrophy, delayed psychomotor development and 3-methylglutaconic aciduria. Treatment of Lamictal combined with Valproate was effective in controlling the seizures.[3]

Interactions

Within the TIM23 complex, the Tim50 subunit directly interacts with TIMM23. The TIM23 complex interacts with the TIMM44 component of the PAM complex and with DNAJC15. An isoform of Tim50 interacts with COIL and snRNPs.[8]

Reference

  1. ↑ 1.0 1.1 Yamamoto H, Esaki M, Kanamori T, Tamura Y, Nishikawa S, Endo T (November 2002). "TIM50 is a subunit of the Tim23 complex which links protein translocation across the outer and inner mitochondrial membranes". Cell. 111 (4): 519–28. doi:10.1016/S0092-8674(02)01053-X. PMID 12437925.
  2. ↑ 2.0 2.1 2.2 2.3 2.4 "Entrez Gene: TIMM50 translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)".
  3. ↑ 3.0 3.1 3.2 Shahrour MA, Staretz-Chacham O, Dayan D, Stephen J, Weech A, Damseh N, et al. (May 2017). "Mitochondrial epileptic encephalopathy, 3-methylglutaconic aciduria and variable complex V deficiency associated with TIMM50 mutations". Clinical Genetics. 91 (5): 690–696. doi:10.1111/cge.12855. PMID 27573165.
  4. ↑ 4.0 4.1 Gao SP, Sun HF, Jiang HL, Li LD, Hu X, Xu XE, Jin W (January 2016). "Loss of TIM50 suppresses proliferation and induces apoptosis in breast cancer". Tumor Biology. 37 (1): 1279–87. doi:10.1007/s13277-015-3878-0. PMID 26289846.
  5. ↑ 5.0 5.1 Tang K, Zhao Y, Li H, Zhu M, Li W, Liu W, et al. (April 2017). "Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy". Journal of the American Heart Association. 6 (4): e004346. doi:10.1161/JAHA.116.004346. PMID 28432072.
  6. ↑ Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, et al. (October 2013). "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research. 113 (9): 1043–53. doi:10.1161/CIRCRESAHA.113.301151. PMC 4076475. PMID 23965338.
  7. ↑ "Mitochondrial import inner membrane translocase subunit TIM50". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB).
  8. ↑ "TIMM50 - Mitochondrial import inner membrane translocase subunit TIM50 precursor - Homo sapiens (Human) - TIMM50 gene & protein". www.uniprot.org. Retrieved 2018-07-25.

Further reading

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

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