Dermatophytosis other diagnostic studies: Difference between revisions

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==Overview==
==Overview==
Most of the time, ringworm can be diagnosed by looking at the skin. The fungus may glow when skin is examined with a blue light (called a Wood's lamp) in a dark room.
Most of the time, ringworm can be diagnosed by looking at the [[skin]]. Other diagnostic studies that can be used to [[diagnose]] dermatophytosis are matrix-assisted laser desorption ionization test and reflectance [[confocal microscopy]].
 
==Other Diagnostic Studies==
 
Other diagnostic studies to confirm dermatophytosis may include:


==Other Diagnostic Tests==
=== Matrix-assisted laser desorption  ===
* Matrix-assisted laser desorption is based on the detection of specific features of a [[Proteolysis|proteolytic]] degradation product which is a product of mycological infections or noninfectious diseases.
* Dermatophytes are represented by [[Proteolysis|proteolytic]] [[degradation]] products of native [[proteins]].
* The [[peptide]] patterns of affected samples are compared with those of known samples containing [[peptide]] spectra from skin disorders stored in an already existing database.
* Matrix-assisted laser desorption is a time efficient process, as it enables simultaneous identification of up to 64 dermatophyte strains, with results coming back within 24 hours.


Tests to confirm the diagnosis may include:
=== Reflectance confocal microscopy ===
* KOH exam
* Reflectance confocal microscopy provides imaging of the [[Epidermis (skin)|epidermis]] and superficial [[dermis]] at a high resolution and can be used to detect [[cutaneous]] [[Fungus|fungi]] and [[parasitic]] infestations.
* Skin biopsy
* Branching [[fungal]] [[hyphae]] can be visualized over an [[erythematous]], annular, [[Scaling skin|scaly]] patch.
* Skin culture


==References==
==References==
{{Reflist|2}}
{{Reflist|2}}
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Latest revision as of 21:18, 29 July 2020

Dermatophytosis Microchapters

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Patient Information

Overview

Historical Perspective

Classification

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Syed Hassan A. Kazmi BSc, MD [2]

Overview

Most of the time, ringworm can be diagnosed by looking at the skin. Other diagnostic studies that can be used to diagnose dermatophytosis are matrix-assisted laser desorption ionization test and reflectance confocal microscopy.

Other Diagnostic Studies

Other diagnostic studies to confirm dermatophytosis may include:

Matrix-assisted laser desorption

  • Matrix-assisted laser desorption is based on the detection of specific features of a proteolytic degradation product which is a product of mycological infections or noninfectious diseases.
  • Dermatophytes are represented by proteolytic degradation products of native proteins.
  • The peptide patterns of affected samples are compared with those of known samples containing peptide spectra from skin disorders stored in an already existing database.
  • Matrix-assisted laser desorption is a time efficient process, as it enables simultaneous identification of up to 64 dermatophyte strains, with results coming back within 24 hours.

Reflectance confocal microscopy

References

Template:WikiDoc Sources