Rotavirus infection laboratory findings: Difference between revisions
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==Overview== | ==Overview== | ||
Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include electron microscopy, reverse transcriptase polymerase chain reaction and RNA electrophoresis. Stool examination for the virus is important to detect the extracted viruses. Also, ELISA and immunochromatographic assays can be used for antigen detection. | Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include [[electron microscopy]], [[Reverse transcription polymerase chain reaction|reverse transcriptase polymerase chain reaction]] and [[RNA]] [[electrophoresis]]. [[Stool examination]] for the virus is important to detect the extracted viruses. Also, [[Enzyme linked immunosorbent assay (ELISA)|ELISA]] and immunochromatographic assays can be used for [[antigen]] detection. | ||
==Laboratory Findings== | ==Laboratory Findings== | ||
*Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue= | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214 }} </ref> | *Specific diagnosis of the [[disease]] is made by identification of the [[virus]] in the patient's [[Stools|stool]]. The most widely used diagnostic laboratory method is [[antigen]] detection in the stool by an [[enzyme immunoassay]] (EIA) directed at an [[antigen]] common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue= | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214 }} </ref> | ||
*Other diagnostic techniques that can be used: | *Other diagnostic techniques that can be used: | ||
**Electron microscopy | **[[Electron microscopy]] | ||
**RNA electrophoresis | **[[RNA]] [[electrophoresis]] | ||
**Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) | **Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) | ||
**Tissue culture | **Tissue culture | ||
*The virus is usually excreted in the stool in the first phases of the infection so, stool examination is required to detect the virus particles. | *The [[virus]] is usually excreted in the [[stool]] in the first phases of the [[infection]] so, [[stool examination]] is required to detect the [[virus]] particles. [[Polyacrylamide gel]] [[electrophoresis]] can be used to detect the [[virus]] in the patients [[stool]]. | ||
*Antigen detecting tests can be used to diagnose the rotavirus infection. It includes: | *[[Antigen]] detecting tests can be used to diagnose the [[rotavirus infection]]. It includes: | ||
**Enzyme Linked Immunosorbent | **[[Enzyme linked immunosorbent assay (ELISA)|Enzyme Linked Immunosorbent Assay (ELISA)]] | ||
**Immunochromatographic assay | **Immunochromatographic assay | ||
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{{reflist|2}} | {{reflist|2}} | ||
[[Category:Disease]] | |||
[[Category:Emergency mdicine]] | |||
[[Category:Up-To-Date]] | |||
[[Category:Infectious disease]] | [[Category:Infectious disease]] | ||
[[Category: | [[Category:Gastroenterology]] | ||
[[Category:Pediatrics]] | |||
Latest revision as of 00:03, 30 July 2020
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Ahmed Elsaiey, MBBCH [2]
Overview
Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include electron microscopy, reverse transcriptase polymerase chain reaction and RNA electrophoresis. Stool examination for the virus is important to detect the extracted viruses. Also, ELISA and immunochromatographic assays can be used for antigen detection.
Laboratory Findings
- Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).[1]
- Other diagnostic techniques that can be used:
- Electron microscopy
- RNA electrophoresis
- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
- Tissue culture
- The virus is usually excreted in the stool in the first phases of the infection so, stool examination is required to detect the virus particles. Polyacrylamide gel electrophoresis can be used to detect the virus in the patients stool.
- Antigen detecting tests can be used to diagnose the rotavirus infection. It includes:
- Enzyme Linked Immunosorbent Assay (ELISA)
- Immunochromatographic assay
References
- ↑ Parashar UD, Nelson EA, Kang G (2013). "Diagnosis, management, and prevention of rotavirus gastroenteritis in children". BMJ. 347: f7204. doi:10.1136/bmj.f7204. PMID 24379214.