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{{WBRQuestion
{{WBRQuestion
|QuestionAuthor=William J Gibson (Reviewed by {{Rim}} and Yazan Daaboul)
|QuestionAuthor=William J Gibson (Reviewed by {{Rim}} and Yazan Daaboul)
|ExamType=USMLE Step 1
|ExamType=USMLE Step 1
|MainCategory=Microbiology, Pathology
|MainCategory=Microbiology, Pathology
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|SubCategory=General Principles, Infectious Disease
|SubCategory=General Principles, Infectious Disease
|Prompt=A microbiologist has been given a number of unknown clinical samples and is attempting to visualize the organisms by gram stain.  Samples from which patient population will he be unable to visualize with this method?
|Prompt=A microbiologist has been given a number of unknown clinical samples and is attempting to visualize the organisms by gram stain.  Samples from which patient population will he be unable to visualize with this method?
|Explanation=''[[Legionella pneumophila]]'' is a primarily intracellular organism which gram-stains poorly. The stain of choice for Legionella is a silver stain. Legionella infections cause [[atypical pneumonia]] and are associated with aerosols from contaminated water, such as certain air conditioning systems or cruises in lakes and rivers.  
|Explanation=''[[Legionella pneumophila]]'' is an intracellular organism which gram-stains poorly. The stain of choice for Legionella is a silver stain. ''Legionella'' causes [[atypical pneumonia]] and is transmitted by aerosols from contaminated water, such as air conditioning systems or cruises in lakes and rivers. ''[[Legionella]]'' infection can cause [[Legionnaires' disease]], which is a severe illness in older individuals. However, it may also cause a milder manifestation termed [[Pontiac fever]]. The treatment of choice for ''Legionella'' infections is a [[macrolide]] (e.g. [[erythromycin]]).
 
''[[Legionella]]'' infection can cause the very severe [[Legionnaires' disease]] in older individuals. A more mild manifestation of ''[[Legionella]]'' infection is [[Pontiac fever]]. The treatment of choice for legionella infections is a [[macrolide]] (e.g. [[erythromycin]]).
|AnswerA=Sexually active college dormitory students
|AnswerA=Sexually active college dormitory students
|AnswerAExp=''[[Nisseria meningitidis]]'' is a [[gram-negative]] dipoloccus.
|AnswerAExp=''[[Nisseria meningitidis]]'' is a [[gram-negative]] dipoloccus.
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|AnswerCExp=''[[Group B streptococcus]]'' is a gram-positive coccus.
|AnswerCExp=''[[Group B streptococcus]]'' is a gram-positive coccus.
|AnswerD=Patients with recent exposure to contaminated air conditioning systems
|AnswerD=Patients with recent exposure to contaminated air conditioning systems
|AnswerDExp=''[[Legionella pneumophila]]'' is a primarily intracellular organism which gram-stains poorly.  The stain of choice for ''Legionella'' is a silver stain.
|AnswerDExp=''[[Legionella pneumophila]]'' is an intracellular organism which gram-stains poorly.  The stain of choice for ''Legionella'' is a silver stain.
|AnswerE=Elderly patients with osteoarthritis who recently underwent a total hip replacement by prosthesis
|AnswerE=Elderly patients with osteoarthritis who recently underwent a total hip replacement by prosthesis
|AnswerEExp=''[[Staphyloccocus epidermidis]]'' is a gram-positive coccus.
|AnswerEExp=''[[Staphyloccocus epidermidis]]'' is a gram-positive coccus.
|EducationalObjectives=''[[Legionella pneumophila]]'' is a primarily intracellular organism which gram-stains poorly. The stain of choice for ''[[Legionella]]'' is a silver stain.
|EducationalObjectives=''[[Legionella pneumophila]]'' is an intracellular organism which gram-stains poorly. The stain of choice for ''[[Legionella]]'' is a silver stain.
|References=First Aid 2014 page 121
|References=First Aid 2014 page 121
|RightAnswer=D
|RightAnswer=D

Latest revision as of 23:10, 27 October 2020

 
Author [[PageAuthor::William J Gibson (Reviewed by Rim Halaby, M.D. [1] and Yazan Daaboul)]]
Exam Type ExamType::USMLE Step 1
Main Category MainCategory::Microbiology, MainCategory::Pathology
Sub Category SubCategory::General Principles, SubCategory::Infectious Disease
Prompt Prompt::A microbiologist has been given a number of unknown clinical samples and is attempting to visualize the organisms by gram stain. Samples from which patient population will he be unable to visualize with this method?
Answer A AnswerA::Sexually active college dormitory students
Answer A Explanation [[AnswerAExp::Nisseria meningitidis is a gram-negative dipoloccus.]]
Answer B AnswerB::Adult patients with a history of alcoholism
Answer B Explanation [[AnswerBExp::Klebsiella pneumoniae is a gram-negative rod.]]
Answer C AnswerC::Infants of pregnant women who were not screened during 3rd trimester
Answer C Explanation [[AnswerCExp::Group B streptococcus is a gram-positive coccus.]]
Answer D AnswerD::Patients with recent exposure to contaminated air conditioning systems
Answer D Explanation [[AnswerDExp::Legionella pneumophila is an intracellular organism which gram-stains poorly. The stain of choice for Legionella is a silver stain.]]
Answer E AnswerE::Elderly patients with osteoarthritis who recently underwent a total hip replacement by prosthesis
Answer E Explanation [[AnswerEExp::Staphyloccocus epidermidis is a gram-positive coccus.]]
Right Answer RightAnswer::D
Explanation [[Explanation::Legionella pneumophila is an intracellular organism which gram-stains poorly. The stain of choice for Legionella is a silver stain. Legionella causes atypical pneumonia and is transmitted by aerosols from contaminated water, such as air conditioning systems or cruises in lakes and rivers. Legionella infection can cause Legionnaires' disease, which is a severe illness in older individuals. However, it may also cause a milder manifestation termed Pontiac fever. The treatment of choice for Legionella infections is a macrolide (e.g. erythromycin).

Educational Objective: Legionella pneumophila is an intracellular organism which gram-stains poorly. The stain of choice for Legionella is a silver stain.
References: First Aid 2014 page 121]]

Approved Approved::Yes
Keyword WBRKeyword::Bacteria, WBRKeyword::Microbiology, WBRKeyword::Legionella, WBRKeyword::Gram stain, WBRKeyword::Stain, WBRKeyword::silver, WBRKeyword::air condition, WBRKeyword::air conditioning
Linked Question Linked::
Order in Linked Questions LinkedOrder::