Cresol Red: Difference between revisions

Jump to navigation Jump to search
m (1 revision)
m (Bot: Automated text replacement (-{{SIB}} + & -{{EH}} + & -{{EJ}} + & -{{Editor Help}} + & -{{Editor Join}} +))
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
[[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]]
{{Chembox new
|  Name = Cresol Red
|  ImageFile = Cresol Red.svg
<!-- | ImageSize = 200px  -->
|  IUPACName =
| Section1 = {{Chembox Identifiers
|   CASNo = 1733-12-6
|   SMILES = Cc1cc(ccc1O)/C(=C/2C=CC(=O)C(=C2)C)c3ccccc3OS(O)=O
  }}
| Section2 = {{Chembox Properties
|  Formula = C<sub>21</sub>H<sub>17</sub>NaO<sub>5</sub>S
|  MolarMass = 404.41 g/mol
|  Density =
|  MeltingPt =
|  BoilingPt =
  }}
}}
{{pH_indicator_template|indicator_name=Cresol  Red|low_pH=7.2|high_pH=8.8|low_pH_color=yellow|high_pH_color=#800080}}
{{SI}}


[[Image:Agarose gel dyes.jpg|right|150px]]


'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs.


== Agarose molecular weight marker ==
'''Cresol Red''' (full name: o-Cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue


* molecular weight 404 g/mol
Cresol Red can be used in many molecular reactions instead of water. For example restriction digestion or PCR.
* in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
* in 3% agarose at >100bp (xylene cyanol 300bp)


== pH indicator ==
Cresol Red can also be used as a color marker to monitor the process of [[agarose gel electrophoresis]] and [[polyacrylamide gel electrophoresis]]. In a 1% agarose gel it runs approximately at the size of a 125 base pair (bp) [[DNA]] molecule (it depends on the concentration of buffer and other component). [[Bromophenol blue]] and [[xylene cyanol]] can also be used for this purpose.
* amber - acidic pH
* yellow - neutral
* red - alkaline


== External links ==
==References==
* [[Image:3stars.png]] [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
<references/>
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue]
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]


[[Category:Material]] [[Category:Chemical]] [[Category:agarose gel electrophoresis]]
 
 
 
[[Category:Triarylmethane dyes]]
 
[[de:Kresolrot]]
[[fr:Rouge de crésol]]
[[it:Rosso cresolo]]
[[ja:クレゾールレッド]]
[[pl:Czerwień krezolowa]]
[[zh:甲酚紅]]
 
 
{{WH}}
{{WS}}

Latest revision as of 00:19, 9 August 2012

Template:Chembox new Template:PH indicator template

WikiDoc Resources for Cresol Red

Articles

Most recent articles on Cresol Red

Most cited articles on Cresol Red

Review articles on Cresol Red

Articles on Cresol Red in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on Cresol Red

Images of Cresol Red

Photos of Cresol Red

Podcasts & MP3s on Cresol Red

Videos on Cresol Red

Evidence Based Medicine

Cochrane Collaboration on Cresol Red

Bandolier on Cresol Red

TRIP on Cresol Red

Clinical Trials

Ongoing Trials on Cresol Red at Clinical Trials.gov

Trial results on Cresol Red

Clinical Trials on Cresol Red at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on Cresol Red

NICE Guidance on Cresol Red

NHS PRODIGY Guidance

FDA on Cresol Red

CDC on Cresol Red

Books

Books on Cresol Red

News

Cresol Red in the news

Be alerted to news on Cresol Red

News trends on Cresol Red

Commentary

Blogs on Cresol Red

Definitions

Definitions of Cresol Red

Patient Resources / Community

Patient resources on Cresol Red

Discussion groups on Cresol Red

Patient Handouts on Cresol Red

Directions to Hospitals Treating Cresol Red

Risk calculators and risk factors for Cresol Red

Healthcare Provider Resources

Symptoms of Cresol Red

Causes & Risk Factors for Cresol Red

Diagnostic studies for Cresol Red

Treatment of Cresol Red

Continuing Medical Education (CME)

CME Programs on Cresol Red

International

Cresol Red en Espanol

Cresol Red en Francais

Business

Cresol Red in the Marketplace

Patents on Cresol Red

Experimental / Informatics

List of terms related to Cresol Red


Cresol Red (full name: o-Cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.

Cresol Red can be used in many molecular reactions instead of water. For example restriction digestion or PCR.

Cresol Red can also be used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.

References


de:Kresolrot it:Rosso cresolo


Template:WH Template:WS