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| __NOTOC__
| | #REDIRECT [[Yersinia pestis]] |
| {{Yersinia pestis infection}}
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| {{CMG}}; {{AE}} Esther Lee, M.A.; {{Rim}}; {{JS}}
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| ==Overview==
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| ''[[Yersinia pestis]]'' (''Y. pestis''), a rod-shaped [[facultative anaerobe]] with bipolar staining (giving it a safety pin appearance) causes the infection in mammals and humans.<ref name=Baron>{{cite book | author = Collins FM | title = Pasteurella, Yersinia, and Francisella. ''In:'' Baron's Medical Microbiology ''(Baron S ''et al'', eds.)| edition = 4th | publisher = Univ. of Texas Medical Branch | year = 1996 | url = http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.section.1611 | isbn = 0-9631172-1-1 }}</ref> The bacteria maintain their existence in a cycle involving rodents and their fleas. The genus Yersinia is [[gram-negative]], bipolar staining coccobacilli, and, similarly to other [[Enterobacteriaceae]], it has a fermentative metabolism. ''Y. pestis'' produces an antiphagocytic slime. The organism is motile when isolated, but becomes nonmotile in the mammalian host.
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| ==Taxonomy==
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| [[Bacteria]]; [[Proteobacteria]]; [[Proteobacteria#Gammaproteobacteria|Gammaproteobacteria]]; [[Enterobacteriales]]; ''[[Yersinia]]''; [[Yersinia pestis]]
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| ==Biology==
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| {| style="float: right;"
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| | [[File:Yersinia pestis fluorescent.jpeg|200px|thumb|none|Yersinia pestis, Direct Fluorescent Antibody Stain (DFA), 200x Magnification <SMALL>Courtesy: ''[http://phil.cdc.gov/phil/home.asp Public Health Image Library (PHIL), Centers for Disease Control and Prevention (CDC)]''<ref>{{Cite web | title = http://phil.cdc.gov/phil/details.asp | url = http://phil.cdc.gov/phil/details.asp}}</ref></SMALL>]]
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| | [[File:Yersinia2.jpg|200px|thumb|none|Photomicrograph of Yersinia (Pasteurella) pestis, sometimes referred to as Bacillus pestis. <SMALL>Courtesy: ''[http://phil.cdc.gov/phil/home.asp Public Health Image Library (PHIL), Centers for Disease Control and Prevention (CDC)]''<ref>{{Cite web | title = http://phil.cdc.gov/phil/details.asp | url = http://phil.cdc.gov/phil/details.asp}}</ref></SMALL>]]
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| Yersinia pests is a nonmotile, non-spore-forming, Gram-negative, non-lactose fermenting, bipolar, ovoid, "safety-pin-shaped" (bipolar appearance when stained) bacillus. It is an [[enterobacteriaceae]] commonly measuring 0.75x1.5 μm. On cell cultures, it grows on sheep-blood agar, as grayish translucent colonies. It also grows on MacConkey and nutrient-rich broths.
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| [[Yersinia pestis]] only survives for a few hours on physical surfaces, being very sensitive to high temperatures, sunlight and disinfectants.<ref name="pmid8993858">{{cite journal| author=Perry RD, Fetherston JD| title=Yersinia pestis--etiologic agent of plague. | journal=Clin Microbiol Rev | year= 1997 | volume= 10 | issue= 1 | pages= 35-66 | pmid=8993858 | doi= | pmc=PMC172914 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8993858 }} </ref><ref name="pmid10852811">{{cite journal| author=Gage KL, Dennis DT, Orloski KA, Ettestad P, Brown TL, Reynolds PJ et al.| title=Cases of cat-associated human plague in the Western US, 1977-1998. | journal=Clin Infect Dis | year= 2000 | volume= 30 | issue= 6 | pages= 893-900 | pmid=10852811 | doi=10.1086/313804 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=10852811 }} </ref><ref name="Koirala2006">{{cite journal|last1=Koirala|first1=Janak|title=Plague: Disease, Management, and Recognition of Act of Terrorism|journal=Infectious Disease Clinics of North America|volume=20|issue=2|year=2006|pages=273–287|issn=08915520|doi=10.1016/j.idc.2006.02.004}}</ref>
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| According to its behavior towards nitrate and glycerol, [[Yersinia pestis]] bay be classified as:
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| * Biovar Antiqua
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| :* Nitrate reduction: positive
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| :* Glycerol reduction: positive
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| * Biovar Medievalis
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| :* Nitrate reduction: negative
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| :* Glycerol use: positive
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| * Biovar Orientalis
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| :* Nitrate reduction: positive
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| :* Glycerol use: negative
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| ===Genome===
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| The complete [[genome|genomic]] sequence is available for two of the three sub-species of yersinia pestis: strain KIM (of biovar Medievalis),<ref>{{cite journal | author = Deng W| title = Genome Sequence of Yersinia pestis KIM | journal = Journal of Bacteriology | year = 2002 | volume = 184 | issue = 16 | pages = 4601–4611 | doi= 10.1128/JB.184.16.4601-4611.2002 | pmid=12142430 | pmc = 135232 | author-separator = , | display-authors = 1 | last2 = Burland | first2 = V. | last3 = Plunkett Iii | first3 = G. | last4 = Boutin | first4 = A. | last5 = Mayhew | first5 = G. F. | last6 = Liss | first6 = P. | last7 = Perna | first7 = N. T. | last8 = Rose | first8 = D. J. | last9 = Mau | first9 = B.}}</ref> and strain CO92 (of biovar Orientalis, obtained from a clinical isolate in the United States).<ref>{{cite journal | author = Parkhill J| title = Genome sequence of Yersinia pestis, the causative agent of plague | journal = Nature | year = 2001 | volume = 413 | issue = 6855| pages = 523–527 | doi = 10.1038/35097083 | pmid = 11586360 | author-separator = , | display-authors = 1 | last2 = Wren | first2 = B. W. | last3 = Thomson | first3 = N. R. | last4 = Titball | first4 = R. W. | last5 = Holden | first5 = M. T. G. | last6 = Prentice | first6 = M. B. | last7 = Sebaihia | first7 = M. | last8 = James | first8 = K. D. | last9 = Churcher | first9 = C.}}</ref> As of 2006, the genomic sequence of a strain of biovar Antiqua has been recently completed.<ref name="pmid16740952">{{cite journal |author=Chain PS |title=Complete Genome Sequence of Yersinia pestis Strains Antiqua and Nepal516: Evidence of Gene Reduction in an Emerging Pathogen |journal=J. Bacteriol. |volume=188 |issue=12 |pages=4453–63 |year=2006 |pmid=16740952 |doi=10.1128/JB.00124-06 |pmc=1482938 |author-separator=, |author2=Hu P |author3=Malfatti SA |display-authors=3 |last4=Radnedge |first4=L. |last5=Larimer |first5=F. |last6=Vergez |first6=L. M. |last7=Worsham |first7=P. |last8=Chu |first8=M. C. |last9=Andersen |first9=G. L.}}</ref> Similar to the other pathogenic strains, there are signs of loss of function mutations. The [[chromosome]] of strain KIM is 4,600,755 base pairs long; the chromosome of strain CO92 is 4,653,728 base pairs long. Like its cousins [[Yersinia pseudotuberculosis|Yersinia pseudotuberculosis]] and [[Yersinia enterocolitica|Yersinia enterocolitica]], Yersinia pestis is host to the [[plasmid]] pCD1. In addition, it also hosts two other plasmids, pPCP1 (also called pPla or pPst) and pMT1 (also called pFra) that are not carried by the other Yersinia species. pFra codes for a [[phospholipase D]] that is important for the ability of Yersinia pestis to be transmitted by fleas. pPla codes for a [[protease]], Pla, that activates [[plasminogen]] in human hosts and is a very important [[virulence factor]] for pneumonic plague.<ref name="pmid17255510">{{cite journal| author=Lathem WW, Price PA, Miller VL, Goldman WE| title=A plasminogen-activating protease specifically controls the development of primary pneumonic plague. | journal=Science | year= 2007 | volume= 315 | issue= 5811 | pages= 509-13 | pmid=17255510 | doi=10.1126/science.1137195 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=17255510 }} </ref> Together, these plasmids, and a [[pathogenicity island]] called HPI, encode several proteins that cause the pathogenesis, for which Yersinia pestis is famous. Among other things, these [[virulence]] factors are required for bacterial adhesion and injection of proteins into the host cell, invasion of bacteria in the host cell (via a Type III secretion system), and acquisition and binding of iron that is harvested from red blood cells (via siderophores). Yersinia pestis is thought to be descendant from Yersinia pseudotuberculosis, differing only in the presence of specific virulence plasmids.
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| A comprehensive and comparative [[proteomics]] analysis of Yersinia pestis strain KIM was performed in 2006.<ref>{{cite journal | author = Hixson K| title = Biomarker candidate identification in Yersinia pestis using organism-wide semiquantitative proteomics | journal = Journal of Proteome Research | year = 2006 | volume = 5 | issue = 11 | pages = 3008–3017 | pmid = 16684765 | doi = 10.1021/pr060179y | author-separator = , | display-authors = 1 | last2 = Adkins | first2 = Joshua N. | last3 = Baker | first3 = Scott E. | last4 = Moore | first4 = Ronald J. | last5 = Chromy | first5 = Brett A. | last6 = Smith | first6 = Richard D. | last7 = McCutchen-Maloney | first7 = Sandra L. | last8 = Lipton | first8 = Mary S. | last9 = Heffron | first9 = F}}</ref> The analysis focused on the transition to a growth condition mimicking growth in host cells.
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| ==Tropism==
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| [[Yersinia pestis]] shows [[tropism]] for [[lymphoid tissue]].
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| ==Natural reservoir==
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| Plague is primarily a disease of rodents. The infection is maintained in natural foci of the disease in wild rodent colonies through transmission between rodents by their flea ectoparasites.
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| ==References==
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| {{Reflist|2}}
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| [[Category:Dermatology]]
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| [[Category:Infectious disease]]
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| [[Category:Pulmonology]]
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| [[Category:Hematology]]
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| [[Category:Disease]]
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| {{WH}}
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| {{WS}}
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