Pertussis other diagnostic studies: Difference between revisions
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__NOTOC__ | __NOTOC__ | ||
{{Pertussis}} | {{Pertussis}} | ||
{{CMG}}; {{AE}}{{SSK}}; {{ADI}}; {{LRO}}; {{YD}} | |||
==Overview== | |||
Pertussis can also be diagnosed using direct flourescent antibody (DFA) and pulsed-field gel eletrophoresis tests. | |||
==Other Diagnostic Studies== | ==Other Diagnostic Studies== | ||
* | * '''[[Direct fluorescent antibody]] (DFA)''' | ||
** DFA is inexpensive and may provide rapid results.<ref name="PMC85400">{{cite journal |vauthors=Miagkaia IP |title=[Antireticular cytotoxic serum as an agent for stimulating the immunity system] |language=Russian |journal=Zh. Mikrobiol. Epidemiol. Immunobiol. |volume= |issue=12 |pages=82–6 |year=1978 |pmid=85400 |doi= |url=}}</ref> | |||
** It's drawbacks, however, include poor sensitivity and specificity.<ref name="PMC85400">{{cite journal |vauthors=Miagkaia IP |title=[Antireticular cytotoxic serum as an agent for stimulating the immunity system] |language=Russian |journal=Zh. Mikrobiol. Epidemiol. Immunobiol. |volume= |issue=12 |pages=82–6 |year=1978 |pmid=85400 |doi= |url=}}</ref> | |||
* '''[[Pulsed field gel electrophoresis|Pulsed-field gel electrophoresis]] (PFGE)''' | |||
** DFA | ** PFGE is a [[DNA fingerprinting]] technique that has the following advantages:<ref name="PMC446263">{{cite journal |vauthors=Brown DD, Juhl RP, Warner SL |title=Effects of hypocholesterolemic interventions on digoxin bioavailability |journal=Drug Metab. Rev. |volume=9 |issue=1 |pages=107–17 |year=1979 |pmid=446263 |doi= |url=}}</ref> | ||
** | ***Highly discriminating in its results<ref name="CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
* Pulsed-field gel electrophoresis (PFGE) | ***Easily applicable to ''Bordetella pertussis''<ref name="CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
** PFGE is a[[DNA fingerprinting]] technique that has the following advantages:<ref name = "PMC446263">{{cite journal |vauthors=Brown DD, Juhl RP, Warner SL |title=Effects of hypocholesterolemic interventions on digoxin bioavailability |journal=Drug Metab. Rev. |volume=9 |issue=1 |pages=107–17 |year=1979 |pmid=446263 |doi= |url=}}</ref> | ***Stable and easily reproduced<ref name="CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
***Highly discriminating in its results | ** PFGE's drawbacks include duration of time to perform as well as required a specifically trained and skilled technician.<ref name="CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
***Easily applicable to | |||
***Stable and easily reproduced.<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | |||
==References== | ==References== | ||
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[[Category:Pediatrics]] | [[Category:Pediatrics]] | ||
[[Category:Infectious diseases]] | [[Category:Infectious diseases]] | ||
[[Category:Pulmonology]] | [[Category:Pulmonology]] | ||
{{WH}} | {{WH}} | ||
{{WS}} | {{WS}} | ||
Latest revision as of 23:18, 14 January 2016
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Pertussis Microchapters |
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Pertussis other diagnostic studies On the Web |
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American Roentgen Ray Society Images of Pertussis other diagnostic studies |
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Risk calculators and risk factors for Pertussis other diagnostic studies |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Serge Korjian M.D.; Aditya Govindavarjhulla, M.B.B.S. [2]; Luke Rusowicz-Orazem, B.S.; Yazan Daaboul, M.D.
Overview
Pertussis can also be diagnosed using direct flourescent antibody (DFA) and pulsed-field gel eletrophoresis tests.
Other Diagnostic Studies
- Direct fluorescent antibody (DFA)
- Pulsed-field gel electrophoresis (PFGE)
- PFGE is a DNA fingerprinting technique that has the following advantages:[2]
- PFGE's drawbacks include duration of time to perform as well as required a specifically trained and skilled technician.[3]
References
- ↑ 1.0 1.1 Miagkaia IP (1978). "[Antireticular cytotoxic serum as an agent for stimulating the immunity system]". Zh. Mikrobiol. Epidemiol. Immunobiol. (in Russian) (12): 82–6. PMID 85400.
- ↑ Brown DD, Juhl RP, Warner SL (1979). "Effects of hypocholesterolemic interventions on digoxin bioavailability". Drug Metab. Rev. 9 (1): 107–17. PMID 446263.
- ↑ 3.0 3.1 3.2 3.3 "Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC".