Xylene cyanol: Difference between revisions

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[[Image:Agarose gel dyes.jpg|right|200px]]
{{Chembox new
|  Name = Xylene cyanol
|  ImageFile = Xylene cyanol.png
<!-- | ImageSize = 200px -->
|  ImageName =
|  IUPACName =
| Section1 = {{Chembox Identifiers
|  CASNo = 2650-17-1
|  SMILES = [Na+].CCNc1ccc(cc1C)/C(=C2/C=C\C(=[NH+]\CC)<br />C(=C2)C)c3ccc(OS([O-])=O)cc3OS([O-])=O
  }}
| Section2 = {{Chembox Properties
|  Formula = C<sub>25</sub>H<sub>27</sub>N<sub>2</sub>O<sub>6</sub>S<sub>2</sub>Na
|  MolarMass = 538.61 g/mol
|  Density =
|   MeltingPt =
|   BoilingPt =
  }}
}}


'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).
'''Xylene cyanol''' can be used as a color marker to monitor the process of [[agarose gel electrophoresis]] and [[polyacrylamide gel electrophoresis]]; in 1% agarose gels, it typically migrates at about the same rate as a 4000 base pair DNA fragment. [[Bromophenol blue]] and [[orange G]] can also be used for this purpose.


* 10'000 bp at 0.7%
Xylene cyanol is also known as '''xylene cyanole''', '''Acid Blue 147''', '''xylene cyanol FF''', '''C.I. 42135''',  ,and sometimes as '''xylene cyanole FF'''.
* 4000 bp at 1.5%
* 750 bp at 2.0%
* 200 bp at 3.0%


== order information ==
==References==
* CAS 2650-17-1


* e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=X4126|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Xylene Cyanol FF X4126] 10g at around €50
<references/>


== see also ==
[[Category:Triarylmethane dyes]]
* [[agarose gel loading buffer]]
* [[agarose gel electrophoresis]]
* [[Cresol Red]] - faster than XC
* [[Bromophenol blue]] - faster than XC


== external links ==
{{biochem-stub}}
* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia]


[[Category:Material]] [[Category:Agarose gel electrophoresis]] [[Category:Dye]]
[[pt:Xileno cianol]]
{{WikiDoc Sources}}

Latest revision as of 21:15, 9 July 2009

Template:Chembox new

Xylene cyanol can be used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis; in 1% agarose gels, it typically migrates at about the same rate as a 4000 base pair DNA fragment. Bromophenol blue and orange G can also be used for this purpose.

Xylene cyanol is also known as xylene cyanole, Acid Blue 147, xylene cyanol FF, C.I. 42135, ,and sometimes as xylene cyanole FF.

References


Template:WikiDoc Sources

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