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<!-- The PBB_Controls template provides controls for Protein Box Bot, please see Template:PBB_Controls for details. -->
{{Infobox_gene}}
{{PBB_Controls
'''Mediator of RNA polymerase II transcription subunit 15''', also known as '''Gal11''','''Spt13''' in yeast and  '''PCQAP''', '''ARC105''', or '''TIG-1''' in humans is a [[protein]] encoded by the ''MED15'' [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: PCQAP PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51586| accessdate = }}</ref>
| update_page = yes
| require_manual_inspection = no
| update_protein_box = yes
| update_summary = yes
| update_citations = yes
}}


<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
== Function ==
{{GNF_Protein_box
| image = PBB_Protein_PCQAP_image.jpg
| image_source = [[Protein_Data_Bank|PDB]] rendering based on 2gut.
| PDB = {{PDB2|2gut}}
| Name = PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein
| HGNCid = 14248
| Symbol = PCQAP
| AltSymbols =; TIG1; ARC105; CAG7A; CTG7A; DKFZp686A2214; DKFZp762B1216; FLJ42282; FLJ42935; MED15; TIG-1; TNRC7
| OMIM = 607372
| ECnumber = 
| Homologene = 32283
| MGIid = 2137379
| GeneAtlas_image1 = PBB_GE_PCQAP_222175_s_at_tn.png
| Function =  
| Component = {{GNF_GO|id=GO:0005634 |text = nucleus}}
| Process = {{GNF_GO|id=GO:0006350 |text = transcription}} {{GNF_GO|id=GO:0006355 |text = regulation of transcription, DNA-dependent}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 51586
    | Hs_Ensembl = ENSG00000099917
    | Hs_RefseqProtein = NP_001003891
    | Hs_RefseqmRNA = NM_001003891
    | Hs_GenLoc_db = 
    | Hs_GenLoc_chr = 22
    | Hs_GenLoc_start = 19191886
    | Hs_GenLoc_end = 19271918
    | Hs_Uniprot = Q96RN5
    | Mm_EntrezGene = 94112
    | Mm_Ensembl = ENSMUSG00000012114
    | Mm_RefseqmRNA = NM_001040683
    | Mm_RefseqProtein = NP_001035773
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 16
    | Mm_GenLoc_start = 17564784
    | Mm_GenLoc_end = 17636460
    | Mm_Uniprot = A0JLM5
  }}
}}
'''PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein''', also known as '''PCQAP''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: PCQAP PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51586| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot. See Template:PBB_Controls to Stop updates. -->
MED15 is a general transcriptional cofactor of the mediator complex involved in RNA polymerase II dependent transcription, originally called Gal11 and Spt13 and found in yeast as an essential factor for '''[[Gal4 transcription factor|Gal4]]''' dependent transactivation by T.Fukasawa and F.Winston labs. Transcription factors Gcn4, Pho4, Msn2, Ino2, members of the '''[[Gal4 transcription factor|Gal4]]''' family - [[Gal4 transcription factor|Gal4]], Oaf1, Pdr1, and viral VP16 have been reported to interact with yeast MED15.<ref>
{{PBB_Summary
{{cite journal | vauthors = Suzuki Y, Nogi Y, Abe A, Fukasawa T | title = GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae | journal = Molecular and Cellular Biology | volume = 8 | issue = 11 | pages = 4991–9 | date = Nov 1988 | pmid = 3062377 | pmc = 365593 | doi =  10.1128/mcb.8.11.4991}};
| section_title =  
{{cite journal | vauthors = Fassler JS, Winston F | title = The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription | journal = Molecular and Cellular Biology | volume = 9 | issue = 12 | pages = 5602–9 | date = Dec 1989 | pmid = 2685570 | pmc = 363730 | doi =  10.1128/mcb.9.12.5602}};
| summary_text = The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene.<ref name="entrez">{{cite web | title = Entrez Gene: PCQAP PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51586| accessdate = }}</ref>
{{cite journal | vauthors = Swanson MJ, Qiu H, Sumibcay L, Krueger A, Kim SJ, Natarajan K, Yoon S, Hinnebusch AG | title = A multiplicity of coactivators is required by Gcn4p at individual promoters in vivo | journal = Molecular and Cellular Biology | volume = 23 | issue = 8 | pages = 2800–20 | date = Apr 2003 | pmid = 12665580 | pmc = 152555 | doi = 10.1128/MCB.23.8.2800-2820.2003 }};
}}
{{cite journal | vauthors = Bryant GO, Ptashne M | title = Independent recruitment in vivo by Gal4 of two complexes required for transcription | journal = Molecular Cell | volume = 11 | issue = 5 | pages = 1301–9 | date = May 2003 | pmid = 12769853 | doi = 10.1016/S1097-2765(03)00144-8 }};
{{cite journal | vauthors = Jedidi I, Zhang F, Qiu H, Stahl SJ, Palmer I, Kaufman JD, Nadaud PS, Mukherjee S, Wingfield PT, Jaroniec CP, Hinnebusch AG | title = Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo | journal = The Journal of Biological Chemistry | volume = 285 | issue = 4 | pages = 2438–55 | date = Jan 2010 | pmid = 19940160 | pmc = 2807301 | doi = 10.1074/jbc.M109.071589 }};
{{cite journal | vauthors = Thakur JK, Arthanari H, Yang F, Chau KH, Wagner G, Näär AM | title = Mediator subunit Gal11p/MED15 is required for fatty acid-dependent gene activation by yeast transcription factor Oaf1p | journal = The Journal of Biological Chemistry | volume = 284 | issue = 7 | pages = 4422–8 | date = Feb 2009 | pmid = 19056732 | doi = 10.1074/jbc.M808263200 | pmc = 3837390 }}
</ref>


==References==
Most of these transcription factors share the same transactivation domain, 9aaTAD, which directly interacts with KIX domain of the MED15.<ref name = "9aaTAD">
{{reflist|2}}
{{cite journal | vauthors = Piskacek S, Gregor M, Nemethova M, Grabner M, Kovarik P, Piskacek M | title = Nine-amino-acid transactivation domain: establishment and prediction utilities | journal = Genomics | volume = 89 | issue = 6 | pages = 756–68 | date = Jun 2007 | pmid = 17467953 | doi = 10.1016/j.ygeno.2007.02.003 }};
==Further reading==
{{cite journal | vauthors = Piskacek M | title = Common Transactivation Motif 9aaTAD recruits multiple general co-activators TAF9, MED15, CBP and p300 | journal = Nature Precedings |date=November 2009 | doi = 10.1038/npre.2009.3488.2 }};
{{cite journal | vauthors = Thakur JK, Arthanari H, Yang F, Chau KH, Wagner G, Näär AM | title = Mediator subunit Gal11p/MED15 is required for fatty acid-dependent gene activation by yeast transcription factor Oaf1p | journal = The Journal of Biological Chemistry | volume = 284 | issue = 7 | pages = 4422–8 | date = Feb 2009 | pmid = 19056732 | doi = 10.1074/jbc.M808263200 | pmc = 3837390 }};
{{cite journal | vauthors = Piskacek M | title = 9aaTAD Prediction result (2006) | journal = Nature Precedings |date=November 2009 | doi = 10.1038/npre.2009.3984.1 }};
{{cite journal | vauthors = Piskacek M | title = Common Transactivation Motif 9aaTAD recruits multiple general co-activators TAF9, MED15, CBP and p300 | journal = Nature Precedings |date=November 2009 | doi = 10.1038/npre.2009.3488.2 }};
{{cite journal | vauthors = Piskacek M | title = 9aaTADs mimic DNA to interact with a pseudo-DNA Binding Domain KIX of Med15 (Molecular Chameleons) | journal = Nature Precedings |date=November 2009 | doi = 10.1038/npre.2009.3939.1 }}
</ref>
 
Furthermore, human MED15 cooperates in mediator complex (previously known as PC2, ARC, or DRIP) with transcription factors like VP16 and  [[sterol regulatory element binding protein|SREBP]]. Human SREBP regulates sterol responsive gene expression, and this regulatory action is conserved in the genetic model organism [[caenorhabditis elegans|''C. elegans'']], a roundworm (homologues MDT-15 and SBP-1). Also in ''C. elegans'', MDT-15 is essential for the response to several stresses (fasting, heavy metal, toxin, and oxidative stress); at least in part the fasting response is conferred by interactions of MDT-15 with [[nuclear receptor]]s, including NHR-49.<ref name="entrez" />
 
== Gene ==
The MED15 gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in [[DiGeorge's syndrome]]. Two transcript variants encoding different isoforms have been found for this gene.<ref name="entrez"/>
 
== References ==
{{reflist}}
 
== Further reading ==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
* {{cite journal | vauthors = Taubert S, Hansen M, Van Gilst MR, Cooper SB, Yamamoto KR | title = The Mediator subunit MDT-15 confers metabolic adaptation to ingested material | journal = PLoS Genetics | volume = 4 | issue = 2 | pages = e1000021 | date = Feb 2008 | pmid = 18454197 | pmc = 2265483 | doi = 10.1371/journal.pgen.1000021 | editor1-last = Mango | editor1-first = Susan E. }} {{open access}}
| citations =  
* {{cite journal | vauthors = Margolis RL, Abraham MR, Gatchell SB, Li SH, Kidwai AS, Breschel TS, Stine OC, Callahan C, McInnis MG, Ross CA | title = cDNAs with long CAG trinucleotide repeats from human brain | journal = Human Genetics | volume = 100 | issue = 1 | pages = 114–22 | date = Jul 1997 | pmid = 9225980 | doi = 10.1007/s004390050476 }}
*{{cite journal | author=Margolis RL, Abraham MR, Gatchell SB, ''et al.'' |title=cDNAs with long CAG trinucleotide repeats from human brain. |journal=Hum. Genet. |volume=100 |issue= 1 |pages= 114-22 |year= 1997 |pmid= 9225980 |doi= }}
* {{cite journal | vauthors = Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R | title = Composite co-activator ARC mediates chromatin-directed transcriptional activation | journal = Nature | volume = 398 | issue = 6730 | pages = 828–32 | date = Apr 1999 | pmid = 10235267 | doi = 10.1038/19789 }}
*{{cite journal | author=Näär AM, Beaurang PA, Zhou S, ''et al.'' |title=Composite co-activator ARC mediates chromatin-directed transcriptional activation. |journal=Nature |volume=398 |issue= 6730 |pages= 828-32 |year= 1999 |pmid= 10235267 |doi= 10.1038/19789 }}
* {{cite journal | vauthors = Taubert S, Van Gilst MR, Hansen M, Yamamoto KR | title = A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans | journal = Genes & Development | volume = 20 | issue = 9 | pages = 1137–49 | date = May 2006 | pmid = 16651656 | pmc = 1472473 | doi = 10.1101/gad.1395406 }}
*{{cite journal | author=Abraham S, Solomon WB |title=A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues. |journal=Gene |volume=255 |issue= 2 |pages= 389-400 |year= 2000 |pmid= 11024300 |doi= }}
* {{cite journal | vauthors = Abraham S, Solomon WB | title = A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues | journal = Gene | volume = 255 | issue = 2 | pages = 389–400 | date = Sep 2000 | pmid = 11024300 | doi = 10.1016/S0378-1119(00)00292-4 }}
*{{cite journal | author=Berti L, Mittler G, Przemeck GK, ''et al.'' |title=Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit. |journal=Genomics |volume=74 |issue= 3 |pages= 320-32 |year= 2001 |pmid= 11414760 |doi= 10.1006/geno.2001.6566 }}
* {{cite journal | vauthors = Berti L, Mittler G, Przemeck GK, Stelzer G, Günzler B, Amati F, Conti E, Dallapiccola B, Hrabé de Angelis M, Novelli G, Meisterernst M | title = Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit | journal = Genomics | volume = 74 | issue = 3 | pages = 320–32 | date = Jun 2001 | pmid = 11414760 | doi = 10.1006/geno.2001.6566 }}
*{{cite journal | author=Kato Y, Habas R, Katsuyama Y, ''et al.'' |title=A component of the ARC/Mediator complex required for TGF beta/Nodal signalling. |journal=Nature |volume=418 |issue= 6898 |pages= 641-6 |year= 2002 |pmid= 12167862 |doi= 10.1038/nature00969 }}
* {{cite journal | vauthors = Kato Y, Habas R, Katsuyama Y, Näär AM, He X | title = A component of the ARC/Mediator complex required for TGF beta/Nodal signalling | journal = Nature | volume = 418 | issue = 6898 | pages = 641–6 | date = Aug 2002 | pmid = 12167862 | doi = 10.1038/nature00969 }}
*{{cite journal | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
* {{cite journal | vauthors = De Luca A, Conti E, Grifone N, Amati F, Spalletta G, Caltagirone C, Bonaviri G, Pasini A, Gennarelli M, Stefano B, Berti L, Mittler G, Meisterernst M, Dallapiccola B, Novelli G | title = Association study between CAG trinucleotide repeats in the PCQAP gene (PC2 glutamine/Q-rich-associated protein) and schizophrenia | journal = American Journal of Medical Genetics Part B | volume = 116B | issue = 1 | pages = 32–5 | date = Jan 2003 | pmid = 12497610 | doi = 10.1002/ajmg.b.10008 }}
*{{cite journal  | author=De Luca A, Conti E, Grifone N, ''et al.'' |title=Association study between CAG trinucleotide repeats in the PCQAP gene (PC2 glutamine/Q-rich-associated protein) and schizophrenia. |journal=Am. J. Med. Genet. B Neuropsychiatr. Genet. |volume=116 |issue= 1 |pages= 32-5 |year= 2003 |pmid= 12497610 |doi= 10.1002/ajmg.b.10008 }}
* {{cite journal | vauthors = Zhang J, Liu L, Pfeifer GP | title = Methylation of the retinoid response gene TIG1 in prostate cancer correlates with methylation of the retinoic acid receptor beta gene | journal = Oncogene | volume = 23 | issue = 12 | pages = 2241–9 | date = Mar 2004 | pmid = 14691453 | doi = 10.1038/sj.onc.1207328 }}
*{{cite journal | author=Zhang J, Liu L, Pfeifer GP |title=Methylation of the retinoid response gene TIG1 in prostate cancer correlates with methylation of the retinoic acid receptor beta gene. |journal=Oncogene |volume=23 |issue= 12 |pages= 2241-9 |year= 2004 |pmid= 14691453 |doi= 10.1038/sj.onc.1207328 }}
* {{cite journal | vauthors = Yang F, DeBeaumont R, Zhou S, Näär AM | title = The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 101 | issue = 8 | pages = 2339–44 | date = Feb 2004 | pmid = 14983011 | pmc = 356952 | doi = 10.1073/pnas.0308676100 }}
*{{cite journal | author=Ota T, Suzuki Y, Nishikawa T, ''et al.'' |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40-5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
* {{cite journal | vauthors = Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC | title = A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology | journal = Molecular Cell | volume = 14 | issue = 5 | pages = 685–91 | date = Jun 2004 | pmid = 15175163 | doi = 10.1016/j.molcel.2004.05.006 }}
*{{cite journal  | author=Yang F, DeBeaumont R, Zhou S, Näär AM |title=The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=101 |issue= 8 |pages= 2339-44 |year= 2004 |pmid= 14983011 |doi= }}
* {{cite journal | vauthors = Sandhu HK, Hollenbeck N, Wassink TH, Philibert RA | title = An association study of PCQAP polymorphisms and schizophrenia | journal = Psychiatric Genetics | volume = 14 | issue = 3 | pages = 169–72 | date = Sep 2004 | pmid = 15318033 | doi = 10.1097/00041444-200409000-00010 }}
*{{cite journal | author=Sato S, Tomomori-Sato C, Parmely TJ, ''et al.'' |title=A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. |journal=Mol. Cell |volume=14 |issue= 5 |pages= 685-91 |year= 2004 |pmid= 15175163 |doi= 10.1016/j.molcel.2004.05.006 }}
* {{cite journal | vauthors = Kwong J, Lo KW, Chow LS, Chan FL, To KF, Huang DP | title = Silencing of the retinoid response gene TIG1 by promoter hypermethylation in nasopharyngeal carcinoma | journal = International Journal of Cancer | volume = 113 | issue = 3 | pages = 386–92 | date = Jan 2005 | pmid = 15455391 | doi = 10.1002/ijc.20593 }}
*{{cite journal | author=Sandhu HK, Hollenbeck N, Wassink TH, Philibert RA |title=An association study of PCQAP polymorphisms and schizophrenia. |journal=Psychiatr. Genet. |volume=14 |issue= 3 |pages= 169-72 |year= 2005 |pmid= 15318033 |doi= }}
* {{cite journal | vauthors = Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I | title = A genome annotation-driven approach to cloning the human ORFeome | journal = Genome Biology | volume = 5 | issue = 10 | pages = R84 | year = 2005 | pmid = 15461802 | pmc = 545604 | doi = 10.1186/gb-2004-5-10-r84 }}
*{{cite journal | author=Kwong J, Lo KW, Chow LS, ''et al.'' |title=Silencing of the retinoid response gene TIG1 by promoter hypermethylation in nasopharyngeal carcinoma. |journal=Int. J. Cancer |volume=113 |issue= 3 |pages= 386-92 |year= 2005 |pmid= 15455391 |doi= 10.1002/ijc.20593 }}
* {{cite journal | vauthors = Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY | title = A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration | journal = Cell | volume = 125 | issue = 4 | pages = 801–14 | date = May 2006 | pmid = 16713569 | doi = 10.1016/j.cell.2006.03.032 }}
*{{cite journal | author=Collins JE, Wright CL, Edwards CA, ''et al.'' |title=A genome annotation-driven approach to cloning the human ORFeome. |journal=Genome Biol. |volume=5 |issue= 10 |pages= R84 |year= 2005 |pmid= 15461802 |doi= 10.1186/gb-2004-5-10-r84 }}
* {{cite journal | vauthors = Yang F, Vought BW, Satterlee JS, Walker AK, Jim Sun ZY, Watts JL, DeBeaumont R, Saito RM, Hyberts SG, Yang S, Macol C, Iyer L, Tjian R, van den Heuvel S, Hart AC, Wagner G, Näär AM | title = An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis | journal = Nature | volume = 442 | issue = 7103 | pages = 700–4 | date = Aug 2006 | pmid = 16799563 | doi = 10.1038/nature04942 }}
*{{cite journal | author=Gerhard DS, Wagner L, Feingold EA, ''et al.'' |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121-7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 }}
* {{cite journal | vauthors = Ishikawa H, Tachikawa H, Miura Y, Takahashi N | title = TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system | journal = FEBS Letters | volume = 580 | issue = 20 | pages = 4784–92 | date = Sep 2006 | pmid = 16904669 | doi = 10.1016/j.febslet.2006.07.066 }}
*{{cite journal  | author=Rual JF, Venkatesan K, Hao T, ''et al.'' |title=Towards a proteome-scale map of the human protein-protein interaction network. |journal=Nature |volume=437 |issue= 7062 |pages= 1173-8 |year= 2005 |pmid= 16189514 |doi= 10.1038/nature04209 }}
*{{cite journal  | author=Lim J, Hao T, Shaw C, ''et al.'' |title=A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. |journal=Cell |volume=125 |issue= 4 |pages= 801-14 |year= 2006 |pmid= 16713569 |doi= 10.1016/j.cell.2006.03.032 }}
*{{cite journal | author=Yang F, Vought BW, Satterlee JS, ''et al.'' |title=An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. |journal=Nature |volume=442 |issue= 7103 |pages= 700-4 |year= 2006 |pmid= 16799563 |doi= 10.1038/nature04942 }}
*{{cite journal | author=Ishikawa H, Tachikawa H, Miura Y, Takahashi N |title=TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system. |journal=FEBS Lett. |volume=580 |issue= 20 |pages= 4784-92 |year= 2006 |pmid= 16904669 |doi= 10.1016/j.febslet.2006.07.066 }}
}}
{{refend}}
{{refend}}
 
{{PDB Gallery|geneid=51586}}
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Latest revision as of 12:26, 9 January 2019

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Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

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Ensembl

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UniProt

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RefSeq (mRNA)

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RefSeq (protein)

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Location (UCSC)n/an/a
PubMed searchn/an/a
Wikidata
View/Edit Human

Mediator of RNA polymerase II transcription subunit 15, also known as Gal11,Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene.[1]

Function

MED15 is a general transcriptional cofactor of the mediator complex involved in RNA polymerase II dependent transcription, originally called Gal11 and Spt13 and found in yeast as an essential factor for Gal4 dependent transactivation by T.Fukasawa and F.Winston labs. Transcription factors Gcn4, Pho4, Msn2, Ino2, members of the Gal4 family - Gal4, Oaf1, Pdr1, and viral VP16 have been reported to interact with yeast MED15.[2]

Most of these transcription factors share the same transactivation domain, 9aaTAD, which directly interacts with KIX domain of the MED15.[3]

Furthermore, human MED15 cooperates in mediator complex (previously known as PC2, ARC, or DRIP) with transcription factors like VP16 and SREBP. Human SREBP regulates sterol responsive gene expression, and this regulatory action is conserved in the genetic model organism C. elegans, a roundworm (homologues MDT-15 and SBP-1). Also in C. elegans, MDT-15 is essential for the response to several stresses (fasting, heavy metal, toxin, and oxidative stress); at least in part the fasting response is conferred by interactions of MDT-15 with nuclear receptors, including NHR-49.[1]

Gene

The MED15 gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge's syndrome. Two transcript variants encoding different isoforms have been found for this gene.[1]

References

  1. ↑ 1.0 1.1 1.2 "Entrez Gene: PCQAP PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein".
  2. ↑ Suzuki Y, Nogi Y, Abe A, Fukasawa T (Nov 1988). "GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae". Molecular and Cellular Biology. 8 (11): 4991–9. doi:10.1128/mcb.8.11.4991. PMC 365593. PMID 3062377.; Fassler JS, Winston F (Dec 1989). "The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription". Molecular and Cellular Biology. 9 (12): 5602–9. doi:10.1128/mcb.9.12.5602. PMC 363730. PMID 2685570.; Swanson MJ, Qiu H, Sumibcay L, Krueger A, Kim SJ, Natarajan K, Yoon S, Hinnebusch AG (Apr 2003). "A multiplicity of coactivators is required by Gcn4p at individual promoters in vivo". Molecular and Cellular Biology. 23 (8): 2800–20. doi:10.1128/MCB.23.8.2800-2820.2003. PMC 152555. PMID 12665580.; Bryant GO, Ptashne M (May 2003). "Independent recruitment in vivo by Gal4 of two complexes required for transcription". Molecular Cell. 11 (5): 1301–9. doi:10.1016/S1097-2765(03)00144-8. PMID 12769853.; Jedidi I, Zhang F, Qiu H, Stahl SJ, Palmer I, Kaufman JD, Nadaud PS, Mukherjee S, Wingfield PT, Jaroniec CP, Hinnebusch AG (Jan 2010). "Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo". The Journal of Biological Chemistry. 285 (4): 2438–55. doi:10.1074/jbc.M109.071589. PMC 2807301. PMID 19940160.; Thakur JK, Arthanari H, Yang F, Chau KH, Wagner G, Näär AM (Feb 2009). "Mediator subunit Gal11p/MED15 is required for fatty acid-dependent gene activation by yeast transcription factor Oaf1p". The Journal of Biological Chemistry. 284 (7): 4422–8. doi:10.1074/jbc.M808263200. PMC 3837390. PMID 19056732.
  3. ↑ Piskacek S, Gregor M, Nemethova M, Grabner M, Kovarik P, Piskacek M (Jun 2007). "Nine-amino-acid transactivation domain: establishment and prediction utilities". Genomics. 89 (6): 756–68. doi:10.1016/j.ygeno.2007.02.003. PMID 17467953.; Piskacek M (November 2009). "Common Transactivation Motif 9aaTAD recruits multiple general co-activators TAF9, MED15, CBP and p300". Nature Precedings. doi:10.1038/npre.2009.3488.2.; Thakur JK, Arthanari H, Yang F, Chau KH, Wagner G, Näär AM (Feb 2009). "Mediator subunit Gal11p/MED15 is required for fatty acid-dependent gene activation by yeast transcription factor Oaf1p". The Journal of Biological Chemistry. 284 (7): 4422–8. doi:10.1074/jbc.M808263200. PMC 3837390. PMID 19056732.; Piskacek M (November 2009). "9aaTAD Prediction result (2006)". Nature Precedings. doi:10.1038/npre.2009.3984.1.; Piskacek M (November 2009). "Common Transactivation Motif 9aaTAD recruits multiple general co-activators TAF9, MED15, CBP and p300". Nature Precedings. doi:10.1038/npre.2009.3488.2.; Piskacek M (November 2009). "9aaTADs mimic DNA to interact with a pseudo-DNA Binding Domain KIX of Med15 (Molecular Chameleons)". Nature Precedings. doi:10.1038/npre.2009.3939.1.

Further reading

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