Trichomoniasis laboratory findings: Difference between revisions
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Laboratory tests used in the diagnosis of trichomoniasis include saline microscopy, culture, and nucleic acid amplification tests (NAATs).<ref name=ggg> Trichomonasias . Wikipedia.https://en.wikipedia.org/wiki/Trichomoniasis Accessed on February 4, 2016</ref> | Laboratory tests used in the diagnosis of trichomoniasis include saline microscopy, culture, and nucleic acid amplification tests (NAATs).<ref name=ggg> Trichomonasias . Wikipedia.https://en.wikipedia.org/wiki/Trichomoniasis Accessed on February 4, 2016</ref> | ||
== Laboratory Findings == | ==Laboratory Findings== | ||
Trichomoniasis is diagnosed by visually observing the trichomonads via a [[microscope]]. In women, the examiner collects the specimen during a [[pelvic examination]] by inserting a [[Speculum (medical)|speculum]] into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor [[Sensitivity|sensitivity]] of the tests | ===Microscopy=== | ||
Trichomoniasis is diagnosed by visually observing the trichomonads via a [[microscope]]. In women, the examiner collects the specimen during a [[pelvic examination]] by inserting a [[Speculum (medical)|speculum]] into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor [[Sensitivity|sensitivity]] of the tests.<ref name=Andrea&Chapin2011>{{cite journal| author=Andrea SB, Chapin KC| title=Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications. | journal=J Clin Microbiol | year= 2011 | volume= 49 | issue= 3 | pages= 866–9 | pmid=21248097 | doi=10.1128/JCM.02367-10 | pmc= | url= | laysummary = http://www.eurekalert.org/pub_releases/2011-03/l-amd030811.php}}</ref> | |||
In men, wet preparation is insensitive, and culture testing of [[urethral]] swab, urine, and [[semen]] is required for optimal sensitivity. No FDA-cleared [[PCR]] test for T. vaginalis is available in the United States, but such testing might be available from commercial laboratories that have developed their own PCR tests.<ref name="urlSTD Facts - Trichomoniasis">{{cite web |url=http://www.cdc.gov/std/trichomonas/STDFact-Trichomoniasis.htm |title=STD Facts - Trichomoniasis |format= |work= |accessdate=2012-12-27}}</ref><ref name="urlVaginal Discharge-STD Treatment Guidelines 2006">{{cite web |url=http://www.cdc.gov/std/treatment/2006/vaginal-discharge.htm#vagdis3 |title=Vaginal Discharge-STD Treatment Guidelines 2006 |format= |work= |accessdate=2012-12-27}}</ref> | In men, wet preparation is insensitive, and culture testing of [[urethral]] swab, urine, and [[semen]] is required for optimal sensitivity. No FDA-cleared [[PCR]] test for T. vaginalis is available in the United States, but such testing might be available from commercial laboratories that have developed their own PCR tests.<ref name="urlSTD Facts - Trichomoniasis">{{cite web |url=http://www.cdc.gov/std/trichomonas/STDFact-Trichomoniasis.htm |title=STD Facts - Trichomoniasis |format= |work= |accessdate=2012-12-27}}</ref><ref name="urlVaginal Discharge-STD Treatment Guidelines 2006">{{cite web |url=http://www.cdc.gov/std/treatment/2006/vaginal-discharge.htm#vagdis3 |title=Vaginal Discharge-STD Treatment Guidelines 2006 |format= |work= |accessdate=2012-12-27}}</ref> | ||
Revision as of 22:16, 4 February 2016
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Maliha Shakil, M.D. [2]
Overview
Laboratory tests used in the diagnosis of trichomoniasis include saline microscopy, culture, and nucleic acid amplification tests (NAATs).[1]
Laboratory Findings
Microscopy
Trichomoniasis is diagnosed by visually observing the trichomonads via a microscope. In women, the examiner collects the specimen during a pelvic examination by inserting a speculum into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor sensitivity of the tests.[2]
In men, wet preparation is insensitive, and culture testing of urethral swab, urine, and semen is required for optimal sensitivity. No FDA-cleared PCR test for T. vaginalis is available in the United States, but such testing might be available from commercial laboratories that have developed their own PCR tests.[3][4]
| Trichomonas vaginalis | Pap smear |
|---|---|
 |
|
Two trophozoites of Trichomonas vaginalis obtained from in vitro culture. Smear was stained with Giemsa.[5]
References
- ↑ Trichomonasias . Wikipedia.https://en.wikipedia.org/wiki/Trichomoniasis Accessed on February 4, 2016
- ↑ Andrea SB, Chapin KC (2011). "Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications". J Clin Microbiol. 49 (3): 866–9. doi:10.1128/JCM.02367-10. PMID 21248097. Lay summary.
- ↑ "STD Facts - Trichomoniasis". Retrieved 2012-12-27.
- ↑ "Vaginal Discharge-STD Treatment Guidelines 2006". Retrieved 2012-12-27.
- ↑ "DPDx - Trichomoniasis". Retrieved 2012-12-27.