Giardiasis laboratory findings: Difference between revisions
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__NOTOC__ | __NOTOC__ | ||
{{Giardiasis}} | {{Giardiasis}} | ||
{{CMG}} {{AE}} {{YD}}; {{SSK}} | |||
{{CMG}} | |||
==Overview== | ==Overview== | ||
The diagnosis of giardiasis requires the detection of ''Giardia'' in at least 1 out of 3 stool samples upon ova and parasite examination. Multiple stool collections (i.e., three stool specimens collected on separate days) should be performed for ova and parasite examination (O & P) to increase test sensitivity since ''Giardia'' cysts are excreted intermittently (detection of apprroximately 80% of cases). Blood samples are usually unremarkable. Fecal immunoassays may also be used for the diagnosis of ''Giardia'' if stool collections were negative and giardiasis is still suspected. Only molecular testing, such as polymerase chain reaction techniques, can be used to identify the subtypes of ''Giardia''. Blood samples are usually unremarkable. | |||
==Laboratory Findings== | ==Laboratory Findings== | ||
===Blood Test=== | |||
*[[ | Blood samples are usually unremarkable. Blood test findings may include the following: | ||
* | *[[Eosinophilia]] (uncommon) | ||
*Elevated anti-''Giardia'' IgM and IgG may distinguish between acute giardiasis (elevated IgM) vs. past infections (elevated IgG) | |||
== | ===Stool Examination=== | ||
*Multiple stool collections (i.e., three stool specimens collected on separate days) should be performed for ova and parasite examination (O & P) to increase test sensitivity since ''Giardia'' cysts are excreted intermittently (detection of apprroximately 80% of cases). | |||
*Fecal immunoassays may also be used for the diagnosis of ''Giardia'' if at least 3 stool collections showed a negative O & P exam and giardiasis is still suspected. Fecal immunoassays are more sensitive and specific than the concentration method and trichrome staining. | |||
*Rapid immune-chromatographic cartridge assays also are available, but should not be ca replacement of the ova and parasite examination. | |||
*Stool culture is not routinely performed due to difficult culturing of the parasite. | |||
===Polymerase Chain Reaction (PCR)=== | |||
*Only molecular testing, such as polymerase chain reaction techniques, can be used to identify the subtypes of ''Giardia''. | |||
==Gallery== | |||
[[Image:giardia.jpg|right|thumb|400px|This picture shows multiple views of a single Giardia lamblia (intestinalis) cyst as imaged at different instrument settings by confocal microscopy.Bar = 10 micrometres.<br />(A) is the cyst imaged by transmission (differential interference contrast), only.<br />(B) is the cyst wall selectively imaged through use of fluorescent-labelled (TRITC) antibody that is cyst wall specific.</br>(C) is the cyst imaged through use of carboxy fluorescein diacetate, a viability stain.<br />(D) is a composite image of (B) and (C).<br />(E) is a composite image of (A), (B), and (C).]] | [[Image:giardia.jpg|right|thumb|400px|This picture shows multiple views of a single Giardia lamblia (intestinalis) cyst as imaged at different instrument settings by confocal microscopy.Bar = 10 micrometres.<br />(A) is the cyst imaged by transmission (differential interference contrast), only.<br />(B) is the cyst wall selectively imaged through use of fluorescent-labelled (TRITC) antibody that is cyst wall specific.</br>(C) is the cyst imaged through use of carboxy fluorescein diacetate, a viability stain.<br />(D) is a composite image of (B) and (C).<br />(E) is a composite image of (A), (B), and (C).]] | ||
Under a normal compound light [[microscope]], ''Giardia'' often looks like a "clown face," with two nuclei outlined by adhesive discs above dark [[median bodies]] that form the "mouth." Cysts have four nuclei. | Under a normal compound light [[microscope]], ''Giardia'' often looks like a "clown face," with two nuclei outlined by adhesive discs above dark [[median bodies]] that form the "mouth." Cysts have four nuclei. | ||
==References== | ==References== | ||
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{{Protozoal diseases}} | {{Protozoal diseases}} | ||
[[Category:Disease]] | [[Category:Disease]] | ||
[[Category:Parasitic diseases]] | [[Category:Parasitic diseases]] | ||
[[Category:Water-borne diseases]] | [[Category:Water-borne diseases]] | ||
[[Category:Infectious disease]] | [[Category:Infectious disease]] | ||
[[Category:Gastroenterology]] | [[Category:Gastroenterology]] | ||
{{WikiDoc Help Menu}} | {{WikiDoc Help Menu}} | ||
{{WikiDoc Sources}} | {{WikiDoc Sources}} | ||
Revision as of 20:41, 9 March 2016
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Giardiasis Microchapters |
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Giardiasis laboratory findings On the Web |
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Risk calculators and risk factors for Giardiasis laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Yazan Daaboul, M.D.; Serge Korjian M.D.
Overview
The diagnosis of giardiasis requires the detection of Giardia in at least 1 out of 3 stool samples upon ova and parasite examination. Multiple stool collections (i.e., three stool specimens collected on separate days) should be performed for ova and parasite examination (O & P) to increase test sensitivity since Giardia cysts are excreted intermittently (detection of apprroximately 80% of cases). Blood samples are usually unremarkable. Fecal immunoassays may also be used for the diagnosis of Giardia if stool collections were negative and giardiasis is still suspected. Only molecular testing, such as polymerase chain reaction techniques, can be used to identify the subtypes of Giardia. Blood samples are usually unremarkable.
Laboratory Findings
Blood Test
Blood samples are usually unremarkable. Blood test findings may include the following:
- Eosinophilia (uncommon)
- Elevated anti-Giardia IgM and IgG may distinguish between acute giardiasis (elevated IgM) vs. past infections (elevated IgG)
Stool Examination
- Multiple stool collections (i.e., three stool specimens collected on separate days) should be performed for ova and parasite examination (O & P) to increase test sensitivity since Giardia cysts are excreted intermittently (detection of apprroximately 80% of cases).
- Fecal immunoassays may also be used for the diagnosis of Giardia if at least 3 stool collections showed a negative O & P exam and giardiasis is still suspected. Fecal immunoassays are more sensitive and specific than the concentration method and trichrome staining.
- Rapid immune-chromatographic cartridge assays also are available, but should not be ca replacement of the ova and parasite examination.
- Stool culture is not routinely performed due to difficult culturing of the parasite.
Polymerase Chain Reaction (PCR)
- Only molecular testing, such as polymerase chain reaction techniques, can be used to identify the subtypes of Giardia.
Gallery
(A) is the cyst imaged by transmission (differential interference contrast), only.
(B) is the cyst wall selectively imaged through use of fluorescent-labelled (TRITC) antibody that is cyst wall specific.
(C) is the cyst imaged through use of carboxy fluorescein diacetate, a viability stain.
(D) is a composite image of (B) and (C).
(E) is a composite image of (A), (B), and (C).
Under a normal compound light microscope, Giardia often looks like a "clown face," with two nuclei outlined by adhesive discs above dark median bodies that form the "mouth." Cysts have four nuclei.