Donovanosis laboratory findings: Difference between revisions
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===Culture=== | ===Culture=== | ||
*''K. granulomatis'' has been successfully cultured in human epithelial (HEp-2) cells with a technique adapted from ''Chlamydia'' culture.<ref name="pmid9350758">{{cite journal| author=Carter J, Hutton S, Sriprakash KS, Kemp DJ, Lum G, Savage J et al.| title=Culture of the causative organism of donovanosis (Calymmatobacterium granulomatis) in HEp-2 cells. | journal=J Clin Microbiol | year= 1997 | volume= 35 | issue= 11 | pages= 2915-7 | pmid=9350758 | doi= | pmc=PMC230086 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=9350758 }} </ref> | |||
*Culturing ''K. granulomatis'' is difficult and not a practical means for diagnosis. | |||
===Polymerase Chain Reaction=== | |||
*A PCR targeting the PhoE gene has been developed but is currently only available as a research tool.<ref name=" O'Farrell"></ref> | |||
===Serology=== | |||
*An indirect immunofluorescence test has been developed but has a low sensitivity and is not practical for diagnosis.<ref name=" O'Farrell"></ref> | |||
==References== | ==References== | ||
Revision as of 18:17, 3 March 2016
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Donovanosis Microchapters |
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Diagnosis |
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Treatment |
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Case Studies |
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Donovanosis laboratory findings On the Web |
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American Roentgen Ray Society Images of Donovanosis laboratory findings |
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Risk calculators and risk factors for Donovanosis laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Kalsang Dolma, M.B.B.S.[2]; Nate Michalak, B.A.
Overview
Laboratory Findings
Microscopy
- Identification of Donovan bodies in tissue smears indicates donovanosis as a strong diagnosis. Donovan bodies are sufficiently unique from other etiologic agents that parasitize macrophages.[1]
- Slides can be created using two methods:[2]
- Tissue smear: after cleaning ulcer surface with saline, a cotton swab is rolled over the ulcer and then rolled over a slide.
- Tissue biopsy: tissue can be obtained from lesion with punch biopsy, forceps, or scalpel and then crushed between two slides. The lesion is often friable but local anesthetic may be necessary.
- Slides are then stained with Giemsa, Leishman's, or Wright's stain to reveal intracellular Donovan bodies within monocytes that may or may not be capsulated.[2]
- Haematoloxylin and eosin are poor stains.
- If swabs are taken for other diagnostic tests, the smear for Donovan bodies should be taken first to ensure there is adequate material to detect the Donovan bodies.
Culture
- K. granulomatis has been successfully cultured in human epithelial (HEp-2) cells with a technique adapted from Chlamydia culture.[3]
- Culturing K. granulomatis is difficult and not a practical means for diagnosis.
Polymerase Chain Reaction
- A PCR targeting the PhoE gene has been developed but is currently only available as a research tool.[2]
Serology
- An indirect immunofluorescence test has been developed but has a low sensitivity and is not practical for diagnosis.[2]
References
- ↑ Richens J (1991). "The diagnosis and treatment of donovanosis (granuloma inguinale)". Genitourin Med. 67 (6): 441–52. PMC 1194766. PMID 1774048.
- ↑ 2.0 2.1 2.2 2.3 O'Farrell N (2002). "Donovanosis". Sex Transm Infect. 78 (6): 452–7. PMC 1758360. PMID 12473810.
- ↑ Carter J, Hutton S, Sriprakash KS, Kemp DJ, Lum G, Savage J; et al. (1997). "Culture of the causative organism of donovanosis (Calymmatobacterium granulomatis) in HEp-2 cells". J Clin Microbiol. 35 (11): 2915–7. PMC 230086. PMID 9350758.