Otitis externa laboratory findings: Difference between revisions
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***Results are purple: indicates that the culture is [[gram-positive]] and is indicative of ''[[Staphylococcus aureus]]''. | ***Results are purple: indicates that the culture is [[gram-positive]] and is indicative of ''[[Staphylococcus aureus]]''. | ||
***Results that are red: indicates that the culture is [[gram-negative]] and is indicative of ''[[Pseudomonas aeruginosa]]''. | ***Results that are red: indicates that the culture is [[gram-negative]] and is indicative of ''[[Pseudomonas aeruginosa]]''. | ||
**[[Polymerase chain reaction|Real-time polymerase chain reaction]] to detect and determine the exact [[bacterial]] pathogen.<ref name="pmid7986047">{{cite journal |vauthors=Khan AA, Cerniglia CE |title=Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR |journal=Appl. Environ. Microbiol. |volume=60 |issue=10 |pages=3739–45 |year=1994 |pmid=7986047 |pmc=201881 |doi= |url=}}</ref> | |||
***Results indicative of ''[[Pseudomonas aeruginosa]]'' or ''[[Staphylococcus aureus]]'' include [[DNA]] samples from either pathogen.<ref name="pmid1629319">{{cite journal |vauthors=Brakstad OG, Aasbakk K, Maeland JA |title=Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene |journal=J. Clin. Microbiol. |volume=30 |issue=7 |pages=1654–60 |year=1992 |pmid=1629319 |pmc=265359 |doi= |url=}}</ref> | |||
==References== | ==References== | ||
Revision as of 13:44, 19 May 2016
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Luke Rusowicz-Orazem, B.S.
Overview
Laboratory Findings
Laboratory findings will vary based on the suspected source of otitis externa.
Bacterial
- Otitis externa from a suspected bacterial infection will be determined by the following tests:
- Swab of the ear canal to determine if the infection is caused by Pseudomonas aeruginosa or Staphylococcus aureus.[1]
- This test will be performed in the event that the symptoms do not resolve with the treatment and the otitis externa becomes recurrent.
- Gram stain test: If the cause is determined to be bacterial, the gram-stain test will determine whether the cause is Psuedomonas aeruginosa or Staphylococcus aureus:[2]
- Results are purple: indicates that the culture is gram-positive and is indicative of Staphylococcus aureus.
- Results that are red: indicates that the culture is gram-negative and is indicative of Pseudomonas aeruginosa.
- Real-time polymerase chain reaction to detect and determine the exact bacterial pathogen.[3]
- Results indicative of Pseudomonas aeruginosa or Staphylococcus aureus include DNA samples from either pathogen.[4]
- Swab of the ear canal to determine if the infection is caused by Pseudomonas aeruginosa or Staphylococcus aureus.[1]
References
- ↑ Llor, C.; McNulty, C. A. M.; Butler, C. C. (2014). "Ordering and interpreting ear swabs in otitis externa". BMJ. 349 (sep01 2): g5259–g5259. doi:10.1136/bmj.g5259. ISSN 1756-1833.
- ↑ Beveridge TJ (2001). "Use of the gram stain in microbiology". Biotech Histochem. 76 (3): 111–8. PMID 11475313.
- ↑ Khan AA, Cerniglia CE (1994). "Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR". Appl. Environ. Microbiol. 60 (10): 3739–45. PMC 201881. PMID 7986047.
- ↑ Brakstad OG, Aasbakk K, Maeland JA (1992). "Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene". J. Clin. Microbiol. 30 (7): 1654–60. PMC 265359. PMID 1629319.