| The NDUFA8 gene is located on the q arm of [[chromosome 9]] in position 33.2 and spans 27,354 base pairs.<ref name = "entrez"/> The gene produces a 20 kDa protein composed of 172 [[amino acids]].<ref name="url_COPaKB">{{cite web | url = http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=P51970 | work = Cardiac Organellar Protein Atlas Knowledgebase (COPaKB) | title = NDUFA8 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 }}</ref> NDUFA8 is a subunit of the enzyme [[NADH dehydrogenase (ubiquinone)]], the largest of the respiratory complexes. The structure is L-shaped with a long, [[hydrophobic]] [[transmembrane]] domain and a [[hydrophilic]] domain for the peripheral arm that includes all the known redox centers and the NADH binding site.<ref name = Biochem /> It has been noted that the [[N-terminus|N-terminal]] hydrophobic domain has the potential to be folded into an [[alpha helix]] spanning the inner [[mitochondrion|mitochondrial membrane]] with a [[C-terminus|C-terminal]] hydrophilic domain interacting with globular subunits of Complex I. The highly [[conserved sequence|conserved]] two-domain structure suggests that this feature is critical for the protein function and that the hydrophobic domain acts as an anchor for the [[NADH dehydrogenase (ubiquinone)]] complex at the inner mitochondrial membrane. NDUFA8 is one of about 31 hydrophobic subunits that form the transmembrane region of Complex I. The predicted [[secondary structure]] is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related [[pseudogenes]] have also been identified on four other chromosomes.<ref name="entrez"/><ref name="pmid9763677"/><ref name="pmid9763676">{{cite journal | vauthors = Emahazion T, Brookes AJ | title = Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping | journal = Cytogenet Cell Genet | volume = 82 | issue = 1–2 | pages = 114 |date=Nov 1998 | pmid = 9763676 | pmc = | doi =10.1159/000015081 }}</ref><ref name="pmid9425316">{{cite journal | vauthors = Ton C, Hwang DM, Dempsey AA, Liew CC | title = Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits | journal = Biochem Biophys Res Commun | volume = 241 | issue = 2 | pages = 589–94 |date=Jan 1998 | pmid = 9425316 | pmc = | doi = 10.1006/bbrc.1997.7707 }}</ref> | | The NDUFA8 gene is located on the q arm of [[chromosome 9]] in position 33.2 and spans 27,354 base pairs.<ref name = "entrez"/> The gene produces a 20 kDa protein composed of 172 [[amino acids]].<ref name=COPaKB>{{cite journal | vauthors = Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, Deng N, Kim AK, Choi JH, Zelaya I, Liem D, Meyer D, Odeberg J, Fang C, Lu HJ, Xu T, Weiss J, Duan H, Uhlen M, Yates JR, Apweiler R, Ge J, Hermjakob H, Ping P | title = Integration of cardiac proteome biology and medicine by a specialized knowledgebase | journal = Circulation Research | volume = 113 | issue = 9 | pages = 1043–53 | date = Oct 2013 | pmid = 23965338 | pmc = 4076475 | doi = 10.1161/CIRCRESAHA.113.301151 }}</ref><ref name="url_COPaKB">{{cite web | url = https://amino.heartproteome.org/web/protein/P51970 | work = Cardiac Organellar Protein Atlas Knowledgebase (COPaKB) | title = NDUFA8 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 }}</ref> NDUFA8 is a subunit of the enzyme [[NADH dehydrogenase (ubiquinone)]], the largest of the respiratory complexes. The structure is L-shaped with a long, [[hydrophobic]] [[transmembrane]] domain and a [[hydrophilic]] domain for the peripheral arm that includes all the known redox centers and the NADH binding site.<ref name = Biochem /> It has been noted that the [[N-terminus|N-terminal]] hydrophobic domain has the potential to be folded into an [[alpha helix]] spanning the inner [[mitochondrion|mitochondrial membrane]] with a [[C-terminus|C-terminal]] hydrophilic domain interacting with globular subunits of Complex I. The highly [[conserved sequence|conserved]] two-domain structure suggests that this feature is critical for the protein function and that the hydrophobic domain acts as an anchor for the [[NADH dehydrogenase (ubiquinone)]] complex at the inner mitochondrial membrane. NDUFA8 is one of about 31 hydrophobic subunits that form the transmembrane region of Complex I. The predicted [[secondary structure]] is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related [[pseudogenes]] have also been identified on four other chromosomes.<ref name="entrez"/><ref name="pmid9763677"/><ref name="pmid9763676">{{cite journal | vauthors = Emahazion T, Brookes AJ | title = Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping | journal = Cytogenet Cell Genet | volume = 82 | issue = 1–2 | pages = 114 |date=Nov 1998 | pmid = 9763676 | pmc = | doi =10.1159/000015081 }}</ref><ref name="pmid9425316">{{cite journal | vauthors = Ton C, Hwang DM, Dempsey AA, Liew CC | title = Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits | journal = Biochem Biophys Res Commun | volume = 241 | issue = 2 | pages = 589–94 |date=Jan 1998 | pmid = 9425316 | pmc = | doi = 10.1006/bbrc.1997.7707 }}</ref> |