RiboGreen: Difference between revisions
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'''RiboGreen''' is a proprietary [[fluorescent]] dye that is used in the detection and quantitation of [[Nucleic acid|nucleic acids]], including both [[DNA]] and [[RNA]]. It is synthesized and marketed by | '''RiboGreen''' is a proprietary [[fluorescent]] dye that is used in the detection and quantitation of [[Nucleic acid|nucleic acids]], including both [[DNA]] and [[RNA]]. It is synthesized and marketed by Molecular Probes (a division of [[Invitrogen]]) of Eugene, Oregon, United States. In its free form, RiboGreen exhibits little fluorescence and possesses a negligible absorbance signature. When bound to nucleic acids, the dye fluoresces with an intensity that, according to the manufacturer, is several orders of magnitude greater than the unbound form. The fluorescence can be detected by a sensor and the nucleic acid can be quantified. The presence of protein contaminants in the sample of nucleic acids to be tested does not make significant contributions to the absorbance, and thus allows for the addition of [[deoxyribonuclease]]s to the protocol in order to degrade DNA, in the instances where one is only interested in detecting or quantifying RNA. | ||
==References== | ==References== | ||
Revision as of 04:13, 6 January 2009
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WikiDoc Resources for RiboGreen |
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Ongoing Trials on RiboGreen at Clinical Trials.gov Clinical Trials on RiboGreen at Google
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US National Guidelines Clearinghouse on RiboGreen
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Patient resources on RiboGreen Discussion groups on RiboGreen Directions to Hospitals Treating RiboGreen Risk calculators and risk factors for RiboGreen
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RiboGreen is a proprietary fluorescent dye that is used in the detection and quantitation of nucleic acids, including both DNA and RNA. It is synthesized and marketed by Molecular Probes (a division of Invitrogen) of Eugene, Oregon, United States. In its free form, RiboGreen exhibits little fluorescence and possesses a negligible absorbance signature. When bound to nucleic acids, the dye fluoresces with an intensity that, according to the manufacturer, is several orders of magnitude greater than the unbound form. The fluorescence can be detected by a sensor and the nucleic acid can be quantified. The presence of protein contaminants in the sample of nucleic acids to be tested does not make significant contributions to the absorbance, and thus allows for the addition of deoxyribonucleases to the protocol in order to degrade DNA, in the instances where one is only interested in detecting or quantifying RNA.