Smallpox laboratory tests: Difference between revisions
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[[Image:Smallpox lab test.jpg]] | [[Image:Smallpox lab test.jpg]] | ||
==Laboratory confirmation=====Laboratory criteria for confirmation=== | |||
*[[Polymerase chain reaction]] (PCR) identification of [[variola]] [[DNA]] in a clinical specimen, OR | |||
*Isolation of smallpox (variola) [[virus]] from a clinical specimen (WHO Smallpox Reference laboratory or laboratory with appropriate reference capabilities) with variola PCR confirmation. | |||
Laboratory diagnostic testing for variola virus should be conducted in a CDC Laboratory Response Network (LRN) laboratory utilizing LRN-approved PCR tests and protocols for variola virus. Initial confirmation of a smallpox outbreak requires additional testing at CDC. | |||
''Note: Generic orthopox PCR and negative stain electron microscopy (EM) identification of a pox virus in a clinical specimen are suggestive of an'' 'orthopox ''' virus infection but not diagnostic for smallpox.'' | |||
The importance of case confirmation using laboratory diagnostic tests differs depending on the epidemiological situation. Because of the low predictive value of a positive lab test result in the absence of a known smallpox outbreak, in the pre-outbreak (pre-event) setting, laboratory testing should be reserved for cases that meet the clinical case definition and are thus classified as being a potential high risk for smallpox. | |||
==Vaccine== | ==Vaccine== | ||
Revision as of 20:44, 6 March 2012
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Smallpox Microchapters |
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Diagnosis |
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Treatment |
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Case Studies |
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Smallpox laboratory tests On the Web |
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American Roentgen Ray Society Images of Smallpox laboratory tests |
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Risk calculators and risk factors for Smallpox laboratory tests |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Michael Maddaleni, B.S.
Overview
This chart describes the laboratory testing protocol for a patient that presents with generalized vesicular or pustular rash illness. It is also based upon the risk level the patient has of developing acquiring smallpox.
Laboratory confirmation=====Laboratory criteria for confirmation=
- Polymerase chain reaction (PCR) identification of variola DNA in a clinical specimen, OR
- Isolation of smallpox (variola) virus from a clinical specimen (WHO Smallpox Reference laboratory or laboratory with appropriate reference capabilities) with variola PCR confirmation.
Laboratory diagnostic testing for variola virus should be conducted in a CDC Laboratory Response Network (LRN) laboratory utilizing LRN-approved PCR tests and protocols for variola virus. Initial confirmation of a smallpox outbreak requires additional testing at CDC.
Note: Generic orthopox PCR and negative stain electron microscopy (EM) identification of a pox virus in a clinical specimen are suggestive of an 'orthopox ' virus infection but not diagnostic for smallpox.
The importance of case confirmation using laboratory diagnostic tests differs depending on the epidemiological situation. Because of the low predictive value of a positive lab test result in the absence of a known smallpox outbreak, in the pre-outbreak (pre-event) setting, laboratory testing should be reserved for cases that meet the clinical case definition and are thus classified as being a potential high risk for smallpox.
Vaccine
The chart below describes a patient that appears to show an adverse reaction to the smallpox vaccine and the protocol involved with it.
