Diagnostic immunology: Difference between revisions
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'''Diagnostic immunology''' is a collective term for a variety of diagnostic techniques that rely on the specificity of the bond between [[antibody|antibodies]] and [[antigen]]es. Diagnostic immunology is well-suited for the detection of even the smallest of amounts of (bio)chemical substances. Antibodies specific for a desired antigen can be conjugated with a [[radiolabel]], fluorescent label, or color-forming enzyme and are used as a "probe" to detect it. | '''Diagnostic immunology''' is a collective term for a variety of diagnostic techniques that rely on the specificity of the bond between [[antibody|antibodies]] and [[antigen]]es. Diagnostic immunology is well-suited for the detection of even the smallest of amounts of (bio)chemical substances. Antibodies specific for a desired antigen can be conjugated with a [[radiolabel]], fluorescent label, or color-forming enzyme and are used as a "probe" to detect it. | ||
Well known applications of this include [[immunoblot]]ting, [[ELISA]] and immunohistochemical staining of microscope slides. The speed, accuracy and simplicity of such tests has led to the development of rapid techniques for the diagnosis of disease, microbes and even illegal drugs ''[[in vivo]]'' (of course tests conducted in a closed environment have a higher degree of accuracy). Such testing is also used to distinguish compatible [[blood type]]s. | ==Examples== | ||
Well-known applications of this include [[immunoblot]]ting, [[ELISA]] and immunohistochemical staining of microscope slides. The speed, accuracy and simplicity of such tests has led to the development of rapid techniques for the diagnosis of disease, microbes and even illegal drugs ''[[in vivo]]'' (of course tests conducted in a closed environment have a higher degree of accuracy). Such testing is also used to distinguish compatible [[blood type]]s. | |||
Immunologic Techniques | |||
* [[Immunoassay]]. "A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance".<ref>{{MeSH|Immunoassay}}</ref> | |||
** [[Radioimmunoassay]] | |||
==See also== | ==See also== | ||
Revision as of 20:32, 22 February 2023
Diagnostic immunology is a collective term for a variety of diagnostic techniques that rely on the specificity of the bond between antibodies and antigenes. Diagnostic immunology is well-suited for the detection of even the smallest of amounts of (bio)chemical substances. Antibodies specific for a desired antigen can be conjugated with a radiolabel, fluorescent label, or color-forming enzyme and are used as a "probe" to detect it.
Examples
Well-known applications of this include immunoblotting, ELISA and immunohistochemical staining of microscope slides. The speed, accuracy and simplicity of such tests has led to the development of rapid techniques for the diagnosis of disease, microbes and even illegal drugs in vivo (of course tests conducted in a closed environment have a higher degree of accuracy). Such testing is also used to distinguish compatible blood types.
Immunologic Techniques
- Immunoassay. "A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance".[1]
See also
- Wikipedia:MeSH E01#MeSH E01.450.495 --- immunologic tests
- Medical technologist
External links
- Immunologic+diagnosis at the US National Library of Medicine Medical Subject Headings (MeSH)
- ↑ Anonymous (2024), Immunoassay (English). Medical Subject Headings. U.S. National Library of Medicine.