Brucellosis laboratory findings: Difference between revisions
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{{Brucellosis}} | {{Brucellosis}} | ||
{{CMG}}; {{AE}} {{RT}} | {{CMG}}; {{AE}} {{RT}} | ||
==Overview== | ==Overview== | ||
Brucellosis is diagnosed in a laboratory by finding Brucella organisms in samples of blood or bone marrow. Also, blood tests can be done to detect antibodies against the bacteria. If this method is used, two blood samples should be collected 2 weeks apart <ref>http://www.cdc.gov/ncidod/dbmd/diseaseinfo/brucellosis_g.htm# </ref>. | Brucellosis is diagnosed in a laboratory by finding [[Brucella]] organisms in samples of blood or [[bone marrow]]. Also, blood tests can be done to detect antibodies against the bacteria. If this method is used, two blood samples should be collected 2 weeks apart <ref>http://www.cdc.gov/ncidod/dbmd/diseaseinfo/brucellosis_g.htm# </ref>. | ||
==Laboratory Findings== | ==Laboratory Findings== | ||
* Complete blood count and differential count - [[leukopenia]], [[anemia]] | * [[Complete blood count]] and differential count - [[leukopenia]], [[anemia]] | ||
* Liver function tests - elevation of AST and ALT | * [[Liver function tests]] - elevation of [[AST]] and [[ALT]] | ||
* Demonstration of the agent: blood cultures in tryptose broth, bone marrow cultures. The growth of brucellae is extremely slow (they can take until 2 months to grow) and the culture poses a risk to laboratory personnel due to high infectivity of brucellae. | * Demonstration of the agent: blood cultures in tryptose broth, bone marrow cultures. The growth of brucellae is extremely slow (they can take until 2 months to grow) and the culture poses a risk to laboratory personnel due to high infectivity of brucellae. | ||
* Demonstration of antibodies against the agent either with the classic Huddleson, Wright and/or Bengal Rose reactions, either with ELISA or the 2-mercaptoethanol assay for IgM antibodies associated with chronic disease | * Demonstration of antibodies against the agent either with the classic Huddleson, Wright and/or Bengal Rose reactions, either with ELISA or the 2-mercaptoethanol assay for IgM antibodies associated with chronic disease | ||
* Histologic evidence of granulomatous hepatitis (hepatic biopsy) | * Histologic evidence of [[granulomatous hepatitis]] (hepatic biopsy) | ||
[[Image:Brucella granuloma.jpg|thumb|left|[[Granuloma]] and [[necrosis]] in the liver of a guinea pig infected with ''Brucella suis'']]{{clr}} | [[Image:Brucella granuloma.jpg|thumb|left|[[Granuloma]] and [[necrosis]] in the liver of a guinea pig infected with ''Brucella suis'']]{{clr}} | ||
==Reference== | ==Reference== | ||
{{reflist|2}} | {{reflist|2}} | ||
[[Category:Bacterial diseases]] | |||
[[Category:Occupational diseases]] | |||
[[Category:Zoonoses]] | |||
[[Category:Infectious disease]] | |||
[[Category:Biological weapons]] | |||
[[Category:Disease]] | |||
{{WH}} | |||
{{WS}} | |||
Revision as of 14:56, 5 December 2012
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Raviteja Guddeti, M.B.B.S. [2]
Overview
Brucellosis is diagnosed in a laboratory by finding Brucella organisms in samples of blood or bone marrow. Also, blood tests can be done to detect antibodies against the bacteria. If this method is used, two blood samples should be collected 2 weeks apart [1].
Laboratory Findings
- Complete blood count and differential count - leukopenia, anemia
- Liver function tests - elevation of AST and ALT
- Demonstration of the agent: blood cultures in tryptose broth, bone marrow cultures. The growth of brucellae is extremely slow (they can take until 2 months to grow) and the culture poses a risk to laboratory personnel due to high infectivity of brucellae.
- Demonstration of antibodies against the agent either with the classic Huddleson, Wright and/or Bengal Rose reactions, either with ELISA or the 2-mercaptoethanol assay for IgM antibodies associated with chronic disease
- Histologic evidence of granulomatous hepatitis (hepatic biopsy)
