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{{Infobox_gene}}
{{PBB_Controls
'''Crossover junction endonuclease MUS81''' is an [[enzyme]] that in humans is encoded by the ''MUS81'' [[gene]].<ref name="pmid11741546">{{cite journal | vauthors = Chen XB, Melchionna R, Denis CM, Gaillard PH, Blasina A, Van de Weyer I, Boddy MN, Russell P, Vialard J, McGowan CH | title = Human Mus81-associated endonuclease cleaves Holliday junctions in vitro | journal = Molecular Cell | volume = 8 | issue = 5 | pages = 1117–27 | date = Nov 2001 | pmid = 11741546 | pmc =  | doi = 10.1016/S1097-2765(01)00375-6 }}</ref><ref name="pmid12374758">{{cite journal | vauthors = Constantinou A, Chen XB, McGowan CH, West SC | title = Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities | journal = The EMBO Journal | volume = 21 | issue = 20 | pages = 5577–85 | date = Oct 2002 | pmid = 12374758 | pmc = 129086 | doi = 10.1093/emboj/cdf554 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: MUS81 MUS81 endonuclease homolog (S. cerevisiae)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=80198| accessdate = }}</ref>
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<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
In mammalian somatic cells, MUS81 and another structure specific DNA endonuclease,  XPF ([[ERCC4]]), play overlapping and essential roles in completion of homologous recombination.<ref name=Kikuchi>{{cite journal |vauthors=Kikuchi K, Narita T, Pham VT, Iijima J, Hirota K, Keka IS, Mohiuddin, Okawa K, Hori T, Fukagawa T, Essers J, Kanaar R, Whitby MC, Sugasawa K, Taniguchi Y, Kitagawa K, Takeda S |title=Structure-specific endonucleases xpf and mus81 play overlapping but essential roles in DNA repair by homologous recombination |journal=Cancer Res. |volume=73 |issue=14 |pages=4362–71 |year=2013 |pmid=23576554 |pmc=3718858 |doi=10.1158/0008-5472.CAN-12-3154 |url=}}</ref>  The significant overlap in function between these enzymes is most likely related to processing joint molecules such as [[D-loop]]s and nicked [[Holliday junctions]].<ref name=Kikuchi />
{{GNF_Protein_box
| image = 
| image_source = 
| PDB =
| Name = MUS81 endonuclease homolog (S. cerevisiae)
| HGNCid = 29814
| Symbol = MUS81
| AltSymbols =; FLJ21012; FLJ44872
| OMIM = 606591
| ECnumber = 
| Homologene = 5725
| MGIid = 1918961
| GeneAtlas_image1 = PBB_GE_MUS81_218463_s_at_tn.png
| Function = {{GNF_GO|id=GO:0000287 |text = magnesium ion binding}} {{GNF_GO|id=GO:0003676 |text = nucleic acid binding}} {{GNF_GO|id=GO:0004518 |text = nuclease activity}} {{GNF_GO|id=GO:0004519 |text = endonuclease activity}} {{GNF_GO|id=GO:0016787 |text = hydrolase activity}} {{GNF_GO|id=GO:0043565 |text = sequence-specific DNA binding}}
| Component = {{GNF_GO|id=GO:0005622 |text = intracellular}} {{GNF_GO|id=GO:0005634 |text = nucleus}}
| Process = {{GNF_GO|id=GO:0006259 |text = DNA metabolic process}} {{GNF_GO|id=GO:0006281 |text = DNA repair}} {{GNF_GO|id=GO:0006310 |text = DNA recombination}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 80198
    | Hs_Ensembl = ENSG00000172732
    | Hs_RefseqProtein = NP_079404
    | Hs_RefseqmRNA = NM_025128
    | Hs_GenLoc_db =
    | Hs_GenLoc_chr = 11
    | Hs_GenLoc_start = 65384525
    | Hs_GenLoc_end = 65390485
    | Hs_Uniprot = Q96NY9
    | Mm_EntrezGene = 71711
    | Mm_Ensembl = ENSMUSG00000024906
    | Mm_RefseqmRNA = NM_027877
    | Mm_RefseqProtein = NP_082153
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 19
    | Mm_GenLoc_start = 5482851
    | Mm_GenLoc_end = 5488339
    | Mm_Uniprot = Q91ZJ0
  }}
}}
'''MUS81 endonuclease homolog (S. cerevisiae)''', also known as '''MUS81''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: MUS81 MUS81 endonuclease homolog (S. cerevisiae)| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=80198| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot. See Template:PBB_Controls to Stop updates. -->
==Meiosis==
{{PBB_Summary
MUS81 is a component of a minor [[chromosomal crossover]] (CO) pathway in the [[meiosis]] of [[Saccharomyces cerevisiae|budding yeast]], plants and vertebrates.<ref name=Lukaszewicz>{{cite journal |vauthors=Lukaszewicz A, Howard-Till RA, Loidl J |title=Mus81 nuclease and Sgs1 helicase are essential for meiotic recombination in a protist lacking a synaptonemal complex |journal=Nucleic Acids Res. |volume=41 |issue=20 |pages=9296–309 |year=2013 |pmid=23935123 |pmc=3814389 |doi=10.1093/nar/gkt703 |url=}}</ref>  However, in the protozoan ''[[Tetrahymena]] thermophila'', MUS81 appears to be part of an essential (if not the predominant) CO pathway.<ref name=Lukaszewicz />  The MUS81 pathway also appears to be the predominant CO pathway in the fission yeast ''[[Schizosaccharomyces pombe]]''.<ref name=Lukaszewicz />
| section_title =  
| summary_text =  
}}


==References==
[[File:Homologous Recombination.jpg|thumb|400px|A current model of meiotic recombination, initiated by a double-strand break or gap, followed by pairing with an homologous chromosome and strand invasion to initiate the recombinational repair process. Repair of the gap can lead to crossover (CO) or non-crossover (NCO) of the flanking regions. CO recombination is thought to occur by the Double Holliday Junction (DHJ) model, illustrated on the right, above. NCO recombinants are thought to occur primarily by the Synthesis Dependent Strand Annealing (SDSA) model, illustrated on the left, above. Most recombination events appear to be the SDSA type.]]
{{reflist|2}}
 
==Further reading==
The relationship of the CO pathway to the overall process of meiotic recombination is illustrated in the accompanying diagram.  [[Genetic recombination|Recombination]] during [[meiosis]] is often initiated by a DNA double-strand break (DSB). During recombination, sections of DNA at the 5' ends of the break are cut away in a process called ''resection''. In the ''strand invasion'' step that follows, an overhanging 3' end of the broken DNA molecule "invades" the DNA of an homologous chromosome that is not broken forming a displacement loop ([[D-loop]]). After strand invasion, the further sequence of events may follow either of two main pathways, leading to a crossover (CO) or a non-crossover (NCO) recombinant (see [[Genetic recombination]]).  The pathway leading to a CO involves a double [[Holliday junction]] (DHJ) intermediate. Holliday junctions need to be resolved for CO recombination to be completed.
 
MU81-MMS4, in the budding yeast ''[[Saccharomyces cerevisiae]]'', is a DNA structure-selective [[endonuclease]] that cleaves joint DNA molecules formed during [[homologous recombination]] in [[meiosis]] and [[mitosis]].<ref name="pmid24744239">{{cite journal |vauthors=Mukherjee S, Wright WD, Ehmsen KT, Heyer WD |title=The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage |journal=Nucleic Acids Res. |volume=42 |issue=10 |pages=6511–22 |year=2014 |pmid=24744239 |pmc=4041439 |doi=10.1093/nar/gku265 |url=}}</ref>  The MUS81-MMS4 endonuclease, although a minor resolvase for CO formation in ''S. cerevisiae'', is crucial for limiting chromosome entanglements by suppressing multiple consecutive recombination events from initiating from the same DSB.<ref name="pmid25329811">{{cite journal |vauthors=Oke A, Anderson CM, Yam P, Fung JC |title=Controlling meiotic recombinational repair - specifying the roles of ZMMs, Sgs1 and Mus81/Mms4 in crossover formation |journal=PLoS Genet. |volume=10 |issue=10 |pages=e1004690 |year=2014 |pmid=25329811 |pmc=4199502 |doi=10.1371/journal.pgen.1004690 |url=}}</ref>
 
Mus81 deficient mice have significant meiotic defects including the failure to repair a subset of DSBs.<ref name="pmid18787696">{{cite journal |vauthors=Holloway JK, Booth J, Edelmann W, McGowan CH, Cohen PE |title=MUS81 generates a subset of MLH1-MLH3-independent crossovers in mammalian meiosis |journal=PLoS Genet. |volume=4 |issue=9 |pages=e1000186 |year=2008 |pmid=18787696 |pmc=2525838 |doi=10.1371/journal.pgen.1000186 |url=}}</ref>
 
== Interactions ==
 
MUS81 has been shown to [[Protein-protein interaction|interact]] with [[CHEK2]].<ref name=pmid11741546 />
 
== References ==
{{reflist}}
{{Clear}}
 
== Further reading ==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
* {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1–2 | pages = 171–4 | date = Jan 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
| citations =
* {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1–2 | pages = 149–56 | date = Oct 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
*{{cite journal | author=Maruyama K, Sugano S |title=Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. |journal=Gene |volume=138 |issue= 1-2 |pages= 171-4 |year= 1994 |pmid= 8125298 |doi= }}
* {{cite journal | vauthors = Boddy MN, Lopez-Girona A, Shanahan P, Interthal H, Heyer WD, Russell P | title = Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1 | journal = Molecular and Cellular Biology | volume = 20 | issue = 23 | pages = 8758–66 | date = Dec 2000 | pmid = 11073977 | pmc = 86503 | doi = 10.1128/MCB.20.23.8758-8766.2000 }}
*{{cite journal | author=Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, ''et al.'' |title=Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. |journal=Gene |volume=200 |issue= 1-2 |pages= 149-56 |year= 1997 |pmid= 9373149 |doi= }}
* {{cite journal | vauthors = Oğrünç M, Sancar A | title = Identification and characterization of human MUS81-MMS4 structure-specific endonuclease | journal = The Journal of Biological Chemistry | volume = 278 | issue = 24 | pages = 21715–20 | date = Jun 2003 | pmid = 12686547 | doi = 10.1074/jbc.M302484200 }}
*{{cite journal | author=Boddy MN, Lopez-Girona A, Shanahan P, ''et al.'' |title=Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1. |journal=Mol. Cell. Biol. |volume=20 |issue= 23 |pages= 8758-66 |year= 2000 |pmid= 11073977 |doi=  }}
* {{cite journal | vauthors = Ciccia A, Constantinou A, West SC | title = Identification and characterization of the human mus81-eme1 endonuclease | journal = The Journal of Biological Chemistry | volume = 278 | issue = 27 | pages = 25172–8 | date = Jul 2003 | pmid = 12721304 | doi = 10.1074/jbc.M302882200 }}
*{{cite journal  | author=Chen XB, Melchionna R, Denis CM, ''et al.'' |title=Human Mus81-associated endonuclease cleaves Holliday junctions in vitro. |journal=Mol. Cell |volume=8 |issue= 5 |pages= 1117-27 |year= 2002 |pmid= 11741546 |doi= }}
* {{cite journal | vauthors = Blais V, Gao H, Elwell CA, Boddy MN, Gaillard PH, Russell P, McGowan CH | title = RNA interference inhibition of Mus81 reduces mitotic recombination in human cells | journal = Molecular Biology of the Cell | volume = 15 | issue = 2 | pages = 552–62 | date = Feb 2004 | pmid = 14617801 | pmc = 329235 | doi = 10.1091/mbc.E03-08-0580 }}
*{{cite journal  | author=Constantinou A, Chen XB, McGowan CH, West SC |title=Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities. |journal=EMBO J. |volume=21 |issue= 20 |pages= 5577-85 |year= 2002 |pmid= 12374758 |doi=  }}
* {{cite journal | vauthors = Gao H, Chen XB, McGowan CH | title = Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cells | journal = Molecular Biology of the Cell | volume = 14 | issue = 12 | pages = 4826–34 | date = Dec 2003 | pmid = 14638871 | pmc = 284787 | doi = 10.1091/mbc.E03-05-0276 }}
*{{cite journal  | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
* {{cite journal | vauthors = Zhang R, Sengupta S, Yang Q, Linke SP, Yanaihara N, Bradsher J, Blais V, McGowan CH, Harris CC | title = BLM helicase facilitates Mus81 endonuclease activity in human cells | journal = Cancer Research | volume = 65 | issue = 7 | pages = 2526–31 | date = Apr 2005 | pmid = 15805243 | doi = 10.1158/0008-5472.CAN-04-2421 }}
*{{cite journal | author=Oğrünç M, Sancar A |title=Identification and characterization of human MUS81-MMS4 structure-specific endonuclease. |journal=J. Biol. Chem. |volume=278 |issue= 24 |pages= 21715-20 |year= 2003 |pmid= 12686547 |doi= 10.1074/jbc.M302484200 }}
* {{cite journal | vauthors = Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M | title = Towards a proteome-scale map of the human protein-protein interaction network | journal = Nature | volume = 437 | issue = 7062 | pages = 1173–8 | date = Oct 2005 | pmid = 16189514 | doi = 10.1038/nature04209 }}
*{{cite journal | author=Ciccia A, Constantinou A, West SC |title=Identification and characterization of the human mus81-eme1 endonuclease. |journal=J. Biol. Chem. |volume=278 |issue= 27 |pages= 25172-8 |year= 2003 |pmid= 12721304 |doi= 10.1074/jbc.M302882200 }}
* {{cite journal | vauthors = Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S | title = Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes | journal = Genome Research | volume = 16 | issue = 1 | pages = 55–65 | date = Jan 2006 | pmid = 16344560 | pmc = 1356129 | doi = 10.1101/gr.4039406 }}
*{{cite journal | author=Blais V, Gao H, Elwell CA, ''et al.'' |title=RNA interference inhibition of Mus81 reduces mitotic recombination in human cells. |journal=Mol. Biol. Cell |volume=15 |issue= 2 |pages= 552-62 |year= 2004 |pmid= 14617801 |doi= 10.1091/mbc.E03-08-0580 }}
* {{cite journal | vauthors = Hiyama T, Katsura M, Yoshihara T, Ishida M, Kinomura A, Tonda T, Asahara T, Miyagawa K | title = Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells | journal = Nucleic Acids Research | volume = 34 | issue = 3 | pages = 880–92 | year = 2006 | pmid = 16456034 | pmc = 1360746 | doi = 10.1093/nar/gkj495 }}
*{{cite journal | author=Gao H, Chen XB, McGowan CH |title=Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cells. |journal=Mol. Biol. Cell |volume=14 |issue= 12 |pages= 4826-34 |year= 2004 |pmid= 14638871 |doi= 10.1091/mbc.E03-05-0276 }}
* {{cite journal | vauthors = Ii M, Ii T, Brill SJ | title = Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae | journal = Mutation Research | volume = 625 | issue = 1–2 | pages = 1–19 | date = Dec 2007 | pmid = 17555773 | pmc = 2100401 | doi = 10.1016/j.mrfmmm.2007.04.007 }}
*{{cite journal | author=Ota T, Suzuki Y, Nishikawa T, ''et al.'' |title=Complete sequencing and characterization of 21,243 full-length human cDNAs. |journal=Nat. Genet. |volume=36 |issue= 1 |pages= 40-5 |year= 2004 |pmid= 14702039 |doi= 10.1038/ng1285 }}
* {{cite journal | vauthors = Nomura Y, Adachi N, Koyama H | title = Human Mus81 and FANCB independently contribute to repair of DNA damage during replication | journal = Genes to Cells | volume = 12 | issue = 10 | pages = 1111–22 | date = Oct 2007 | pmid = 17903171 | doi = 10.1111/j.1365-2443.2007.01124.x }}
*{{cite journal  | author=Gerhard DS, Wagner L, Feingold EA, ''et al.'' |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121-7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 }}
*{{cite journal  | author=Zhang R, Sengupta S, Yang Q, ''et al.'' |title=BLM helicase facilitates Mus81 endonuclease activity in human cells. |journal=Cancer Res. |volume=65 |issue= 7 |pages= 2526-31 |year= 2005 |pmid= 15805243 |doi= 10.1158/0008-5472.CAN-04-2421 }}
*{{cite journal | author=Rual JF, Venkatesan K, Hao T, ''et al.'' |title=Towards a proteome-scale map of the human protein-protein interaction network. |journal=Nature |volume=437 |issue= 7062 |pages= 1173-8 |year= 2005 |pmid= 16189514 |doi= 10.1038/nature04209 }}
*{{cite journal | author=Kimura K, Wakamatsu A, Suzuki Y, ''et al.'' |title=Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. |journal=Genome Res. |volume=16 |issue= 1 |pages= 55-65 |year= 2006 |pmid= 16344560 |doi= 10.1101/gr.4039406 }}
*{{cite journal | author=Hiyama T, Katsura M, Yoshihara T, ''et al.'' |title=Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells. |journal=Nucleic Acids Res. |volume=34 |issue= 3 |pages= 880-92 |year= 2006 |pmid= 16456034 |doi= 10.1093/nar/gkj495 }}
*{{cite journal | author=Ii M, Ii T, Brill SJ |title=Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae. |journal=Mutat. Res. |volume=625 |issue= 1-2 |pages= 1-19 |year= 2007 |pmid= 17555773 |doi= 10.1016/j.mrfmmm.2007.04.007 }}
*{{cite journal | author=Nomura Y, Adachi N, Koyama H |title=Human Mus81 and FANCB independently contribute to repair of DNA damage during replication. |journal=Genes Cells |volume=12 |issue= 10 |pages= 1111-22 |year= 2007 |pmid= 17903171 |doi= 10.1111/j.1365-2443.2007.01124.x }}
}}
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Latest revision as of 07:06, 4 September 2017

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Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

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Ensembl

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UniProt

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RefSeq (mRNA)

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RefSeq (protein)

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Location (UCSC)n/an/a
PubMed searchn/an/a
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View/Edit Human

Crossover junction endonuclease MUS81 is an enzyme that in humans is encoded by the MUS81 gene.[1][2][3]

In mammalian somatic cells, MUS81 and another structure specific DNA endonuclease, XPF (ERCC4), play overlapping and essential roles in completion of homologous recombination.[4] The significant overlap in function between these enzymes is most likely related to processing joint molecules such as D-loops and nicked Holliday junctions.[4]

Meiosis

MUS81 is a component of a minor chromosomal crossover (CO) pathway in the meiosis of budding yeast, plants and vertebrates.[5] However, in the protozoan Tetrahymena thermophila, MUS81 appears to be part of an essential (if not the predominant) CO pathway.[5] The MUS81 pathway also appears to be the predominant CO pathway in the fission yeast Schizosaccharomyces pombe.[5]

File:Homologous Recombination.jpg
A current model of meiotic recombination, initiated by a double-strand break or gap, followed by pairing with an homologous chromosome and strand invasion to initiate the recombinational repair process. Repair of the gap can lead to crossover (CO) or non-crossover (NCO) of the flanking regions. CO recombination is thought to occur by the Double Holliday Junction (DHJ) model, illustrated on the right, above. NCO recombinants are thought to occur primarily by the Synthesis Dependent Strand Annealing (SDSA) model, illustrated on the left, above. Most recombination events appear to be the SDSA type.

The relationship of the CO pathway to the overall process of meiotic recombination is illustrated in the accompanying diagram. Recombination during meiosis is often initiated by a DNA double-strand break (DSB). During recombination, sections of DNA at the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule "invades" the DNA of an homologous chromosome that is not broken forming a displacement loop (D-loop). After strand invasion, the further sequence of events may follow either of two main pathways, leading to a crossover (CO) or a non-crossover (NCO) recombinant (see Genetic recombination). The pathway leading to a CO involves a double Holliday junction (DHJ) intermediate. Holliday junctions need to be resolved for CO recombination to be completed.

MU81-MMS4, in the budding yeast Saccharomyces cerevisiae, is a DNA structure-selective endonuclease that cleaves joint DNA molecules formed during homologous recombination in meiosis and mitosis.[6] The MUS81-MMS4 endonuclease, although a minor resolvase for CO formation in S. cerevisiae, is crucial for limiting chromosome entanglements by suppressing multiple consecutive recombination events from initiating from the same DSB.[7]

Mus81 deficient mice have significant meiotic defects including the failure to repair a subset of DSBs.[8]

Interactions

MUS81 has been shown to interact with CHEK2.[1]

References

  1. ↑ 1.0 1.1 Chen XB, Melchionna R, Denis CM, Gaillard PH, Blasina A, Van de Weyer I, Boddy MN, Russell P, Vialard J, McGowan CH (Nov 2001). "Human Mus81-associated endonuclease cleaves Holliday junctions in vitro". Molecular Cell. 8 (5): 1117–27. doi:10.1016/S1097-2765(01)00375-6. PMID 11741546.
  2. ↑ Constantinou A, Chen XB, McGowan CH, West SC (Oct 2002). "Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities". The EMBO Journal. 21 (20): 5577–85. doi:10.1093/emboj/cdf554. PMC 129086. PMID 12374758.
  3. ↑ "Entrez Gene: MUS81 MUS81 endonuclease homolog (S. cerevisiae)".
  4. ↑ 4.0 4.1 Kikuchi K, Narita T, Pham VT, Iijima J, Hirota K, Keka IS, Mohiuddin, Okawa K, Hori T, Fukagawa T, Essers J, Kanaar R, Whitby MC, Sugasawa K, Taniguchi Y, Kitagawa K, Takeda S (2013). "Structure-specific endonucleases xpf and mus81 play overlapping but essential roles in DNA repair by homologous recombination". Cancer Res. 73 (14): 4362–71. doi:10.1158/0008-5472.CAN-12-3154. PMC 3718858. PMID 23576554.
  5. ↑ 5.0 5.1 5.2 Lukaszewicz A, Howard-Till RA, Loidl J (2013). "Mus81 nuclease and Sgs1 helicase are essential for meiotic recombination in a protist lacking a synaptonemal complex". Nucleic Acids Res. 41 (20): 9296–309. doi:10.1093/nar/gkt703. PMC 3814389. PMID 23935123.
  6. ↑ Mukherjee S, Wright WD, Ehmsen KT, Heyer WD (2014). "The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage". Nucleic Acids Res. 42 (10): 6511–22. doi:10.1093/nar/gku265. PMC 4041439. PMID 24744239.
  7. ↑ Oke A, Anderson CM, Yam P, Fung JC (2014). "Controlling meiotic recombinational repair - specifying the roles of ZMMs, Sgs1 and Mus81/Mms4 in crossover formation". PLoS Genet. 10 (10): e1004690. doi:10.1371/journal.pgen.1004690. PMC 4199502. PMID 25329811.
  8. ↑ Holloway JK, Booth J, Edelmann W, McGowan CH, Cohen PE (2008). "MUS81 generates a subset of MLH1-MLH3-independent crossovers in mammalian meiosis". PLoS Genet. 4 (9): e1000186. doi:10.1371/journal.pgen.1000186. PMC 2525838. PMID 18787696.

Further reading

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