Schistosomiasis laboratory findings: Difference between revisions
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==Laboratory Findings== | ==Laboratory Findings== | ||
Visualization of schistosoma eggs in stool, urine, and crushed biopsy tissues is diagnostic of schistosomiasis. Laboratory findings consistent with the diagnosis of schistosomiasis include detection of circulating antibodies to schistosomes and schistosomal antigen in serum. Diagnostic tests for schistosomiasis include the following: | |||
*Microscopic examination of stool | |||
*Urine testing for schistosome eggs | |||
*Serologic testing | |||
*Schistosomal antigen testing (urine or serum) | |||
*Microscopic examination of tissue | |||
*PCR to detect schistosomal DNA | |||
Eggs can be present in the stool in infections with all ''Schistosoma'' species. The examination can be performed on a simple smear (1 to 2 mg of fecal material). Since eggs may be passed intermittently or in small amounts, their detection will be enhanced by repeated examinations and/or concentration procedures (such as the formalin-ethyl acetate technique). In addition, for field surveys and investigational purposes, the egg output can be quantified by using the [[Kato-Katz technique]] (20 to 50 mg of fecal material) or the Ritchie technique. | Eggs can be present in the stool in infections with all ''Schistosoma'' species. The examination can be performed on a simple smear (1 to 2 mg of fecal material). Since eggs may be passed intermittently or in small amounts, their detection will be enhanced by repeated examinations and/or concentration procedures (such as the formalin-ethyl acetate technique). In addition, for field surveys and investigational purposes, the egg output can be quantified by using the [[Kato-Katz technique]] (20 to 50 mg of fecal material) or the Ritchie technique. | ||
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The eggs of ''S. haematobium'' are ellipsoidal with a terminal spine, ''S. mansoni'' eggs are also ellipsoidal but with a lateral spine, ''S. japonicum'' eggs are spheroidal with a small knob. | The eggs of ''S. haematobium'' are ellipsoidal with a terminal spine, ''S. mansoni'' eggs are also ellipsoidal but with a lateral spine, ''S. japonicum'' eggs are spheroidal with a small knob. | ||
==References== | ==References== | ||
Revision as of 21:28, 10 August 2017
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
Microscopic identification of eggs in stool or urine is the most practical method for diagnosis. The stool exam is the more common of the two. For the measurement of eggs in the feces of presenting patients the scientific unit used is epg or eggs per gram. Stool examination should be performed when infection with S. mansoni or S. japonicum is suspected, and urine examination should be performed if S. haematobium is suspected.
Laboratory Findings
Visualization of schistosoma eggs in stool, urine, and crushed biopsy tissues is diagnostic of schistosomiasis. Laboratory findings consistent with the diagnosis of schistosomiasis include detection of circulating antibodies to schistosomes and schistosomal antigen in serum. Diagnostic tests for schistosomiasis include the following:
- Microscopic examination of stool
- Urine testing for schistosome eggs
- Serologic testing
- Schistosomal antigen testing (urine or serum)
- Microscopic examination of tissue
- PCR to detect schistosomal DNA
Eggs can be present in the stool in infections with all Schistosoma species. The examination can be performed on a simple smear (1 to 2 mg of fecal material). Since eggs may be passed intermittently or in small amounts, their detection will be enhanced by repeated examinations and/or concentration procedures (such as the formalin-ethyl acetate technique). In addition, for field surveys and investigational purposes, the egg output can be quantified by using the Kato-Katz technique (20 to 50 mg of fecal material) or the Ritchie technique.
Eggs can be found in the urine in infections with (recommended time for collection: between noon and 3 PM) S. japonicum' and with S. intercalatum. Detection will be enhanced by centrifugation and examination of the sediment. Quantification is possible by using filtration through a nucleopore membrane of a standard volume of urine followed by egg counts on the membrane. Investigation of S. haematobium should also include a pelvic x-ray as bladder wall calcificaition is highly characteristic of chronic infection.
Recently a field evaluation of a novel handheld microscope was undertaken in Uganda for the diagnosis of intestinal schistosomiasis by a team led by Dr. Russell Stothard who heads the Schistosomiasis Control Iniative at the Natural History Museum, London. His report abstract may be found here: [2]
Tissue biopsy (rectal biopsy for all species and biopsy of the bladder for S. haematobium) may demonstrate eggs when stool or urine examinations are negative.
The eggs of S. haematobium are ellipsoidal with a terminal spine, S. mansoni eggs are also ellipsoidal but with a lateral spine, S. japonicum eggs are spheroidal with a small knob.