Brucellosis laboratory findings: Difference between revisions
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[[Image:Brucella granuloma.jpg|thumb|center|400px|[[Granuloma]] and [[necrosis]] in the liver of a guinea pig infected with ''Brucella suis'']] | [[Image:Brucella granuloma.jpg|thumb|center|400px|[[Granuloma]] and [[necrosis]] in the liver of a guinea pig infected with ''Brucella suis'']] | ||
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==Gallery== | |||
<gallery> | |||
Image: Brucellosis12.jpeg| Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis11.jpeg| Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis10.jpeg| Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis09.jpeg|Brucella suis bacteria grown on chocolate medium 72hrs. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis08.jpeg|Brucella sis bacteria grown on chocolate medium 72hrs. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis07.jpeg|Brucella bacteria grown on MacConkey agar (MAC) medium 24hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis06.jpeg|Brucella suis bacteria cultured on chocolate agar medium 48hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis05.jpeg|Brucella suis bacteria cultured on SBA 24hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis04.jpeg|Brucella suis bacteria cultured on SBA 72hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis03.jpeg|Brucella suis bacteria cultured on SBA 48hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis02.jpeg|Brucella suis bacteria cultured on Thayer-Martin (TM) agar medium 48hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
Image: Brucellosis02.jpeg|Brucella suis bacteria cultured on chocolate agar medium 24hrs (10x mag). <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL> | |||
</gallery> | |||
==References== | ==References== | ||
Revision as of 19:48, 16 June 2015
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Brucellosis Microchapters |
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Diagnosis |
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Treatment |
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Case Studies |
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Brucellosis laboratory findings On the Web |
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American Roentgen Ray Society Images of Brucellosis laboratory findings |
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Risk calculators and risk factors for Brucellosis laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Raviteja Guddeti, M.B.B.S. [2]
Overview
Brucellosis is diagnosed in a laboratory by finding Brucella organisms in samples of blood or bone marrow. Also, blood tests can be done to detect antibodies against the bacteria. If this method is used, two blood samples should be collected 2 weeks apart [1].
Laboratory Findings
- Complete blood count and differential count - leukopenia, anemia
- Liver function tests - elevation of AST and ALT
- Demonstration of the agent: blood cultures in tryptose broth, bone marrow cultures. The growth of brucellae is extremely slow (they can take until 2 months to grow) and the culture poses a risk to laboratory personnel due to high infectivity of brucellae.
- Demonstration of antibodies against the agent either with the classic Huddleson, Wright and/or Bengal Rose reactions, either with ELISA or the 2-mercaptoethanol assay for IgM antibodies associated with chronic disease
- Histologic evidence of granulomatous hepatitis (hepatic biopsy)
Gallery
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![Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]](/images/7/76/Brucellosis12.jpeg)
Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]
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![Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]](/images/8/88/Brucellosis11.jpeg)
Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]
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![Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]](/images/7/71/Brucellosis10.jpeg)
Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]
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![Brucella suis bacteria grown on chocolate medium 72hrs. From Public Health Image Library (PHIL). [2]](/images/0/0e/Brucellosis09.jpeg)
Brucella suis bacteria grown on chocolate medium 72hrs. From Public Health Image Library (PHIL). [2]
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![Brucella sis bacteria grown on chocolate medium 72hrs. From Public Health Image Library (PHIL). [2]](/images/4/4e/Brucellosis08.jpeg)
Brucella sis bacteria grown on chocolate medium 72hrs. From Public Health Image Library (PHIL). [2]
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![Brucella bacteria grown on MacConkey agar (MAC) medium 24hrs (10x mag). From Public Health Image Library (PHIL). [2]](/images/4/4c/Brucellosis07.jpeg)
Brucella bacteria grown on MacConkey agar (MAC) medium 24hrs (10x mag). From Public Health Image Library (PHIL). [2]
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![Brucella suis bacteria cultured on chocolate agar medium 48hrs (10x mag). From Public Health Image Library (PHIL). [2]](/images/6/6d/Brucellosis06.jpeg)
Brucella suis bacteria cultured on chocolate agar medium 48hrs (10x mag). From Public Health Image Library (PHIL). [2]
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![Brucella suis bacteria cultured on SBA 24hrs (10x mag). From Public Health Image Library (PHIL). [2]](/images/b/bb/Brucellosis05.jpeg)
Brucella suis bacteria cultured on SBA 24hrs (10x mag). From Public Health Image Library (PHIL). [2]
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![Brucella suis bacteria cultured on SBA 72hrs (10x mag). From Public Health Image Library (PHIL). [2]](/images/2/23/Brucellosis04.jpeg)
Brucella suis bacteria cultured on SBA 72hrs (10x mag). From Public Health Image Library (PHIL). [2]
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![Brucella suis bacteria cultured on SBA 48hrs (10x mag). From Public Health Image Library (PHIL). [2]](/images/d/d0/Brucellosis03.jpeg)
Brucella suis bacteria cultured on SBA 48hrs (10x mag). From Public Health Image Library (PHIL). [2]
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![Brucella suis bacteria cultured on Thayer-Martin (TM) agar medium 48hrs (10x mag). From Public Health Image Library (PHIL). [2]](/images/4/45/Brucellosis02.jpeg)
Brucella suis bacteria cultured on Thayer-Martin (TM) agar medium 48hrs (10x mag). From Public Health Image Library (PHIL). [2]
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Brucella suis bacteria cultured on chocolate agar medium 24hrs (10x mag). From Public Health Image Library (PHIL). [2]
![Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis12.jpeg)
![Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis11.jpeg)
![Brucella abortus bacteria grown on a medium of sheep’s blood agar (SBA) 72hrs. From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis10.jpeg)
![Brucella suis bacteria grown on chocolate medium 72hrs. From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis09.jpeg)
![Brucella sis bacteria grown on chocolate medium 72hrs. From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis08.jpeg)
![Brucella bacteria grown on MacConkey agar (MAC) medium 24hrs (10x mag). From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis07.jpeg)
![Brucella suis bacteria cultured on chocolate agar medium 48hrs (10x mag). From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis06.jpeg)
![Brucella suis bacteria cultured on SBA 24hrs (10x mag). From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis05.jpeg)
![Brucella suis bacteria cultured on SBA 72hrs (10x mag). From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis04.jpeg)
![Brucella suis bacteria cultured on SBA 48hrs (10x mag). From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis03.jpeg)
![Brucella suis bacteria cultured on Thayer-Martin (TM) agar medium 48hrs (10x mag). From Public Health Image Library (PHIL). [2]](/index.php/File:Brucellosis02.jpeg)