Measles other diagnostic studies: Difference between revisions
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==Overview== | |||
==Vero/hSLAM Cells for Isolation of Measles Virus== | |||
The availability of a sensitive cell line for isolation of measles virus from clinical specimens and establishment of RT-PCR and automated DNA sequencing techniques have allowed for rapid genetic characterization of a large number of wild-type strains of [[measles virus]]. A database of sequence information now makes it possible to use molecular epidemiological techniques to identify the source of wild-type viruses and to differentiate between wild type and vaccine strains. | |||
The Vero/hSLAM cell line [hSLAM=(human) signaling lymphocytic activation molecule] is recommended for use in the laboratories that provide measles surveillance as part of the World Health Organization's Measles and Rubella Laboratory Network (WHO LabNet).<ref name="FeatherstoneRota2011">{{cite journal|last1=Featherstone|first1=D. A.|last2=Rota|first2=P. A.|last3=Icenogle|first3=J.|last4=Mulders|first4=M. N.|last5=Jee|first5=Y.|last6=Ahmed|first6=H.|last7=Bispo de Filippis|first7=A. M.|last8=Ramamurty|first8=N.|last9=Gavrilin|first9=E.|last10=Byabamazima|first10=C.|last11=Dosseh|first11=A.|last12=Xu|first12=W.|last13=Komase|first13=K.|last14=Tashiro|first14=M.|last15=Brown|first15=D.|last16=Bellini|first16=W. J.|last17=Strebel|first17=P.|title=Expansion of the Global Measles and Rubella Laboratory Network 2005-09|journal=Journal of Infectious Diseases|volume=204|issue=Supplement 1|year=2011|pages=S491–S498|issn=0022-1899|doi=10.1093/infdis/jir107}}</ref> | |||
The Vero/hSLAM cells are Vero cells that have been stably transfected with a plasmid encoding the gene for the human (h) SLAM molecule. SLAM (signaling lymphocyte activation molecule) has been shown to be a receptor for both wild-type and laboratory-adapted strains of measles. The sensitivity of Vero/hSLAM cells for isolation of measles virus is equivalent to that of B95a cells. The advantage to the Vero/hSLAM cells is that, unlike the B95a cells, they are not persistently infected with virus. This provides a significant safety advantage for laboratorians and greatly facilitates international shipments. The disadvantage of the Vero/hSLAM cells is that they must be cultured in medium containing geneticin to retain SLAM expression. This increases the cost of the tissue culture medium. | |||
==Genetic Analysis of Measles Viruses== | |||
==References== | ==References== | ||
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[[Category:Pediatrics]] | [[Category:Pediatrics]] | ||
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Alejandro Lemor, M.D. [2]
Overview
Vero/hSLAM Cells for Isolation of Measles Virus
The availability of a sensitive cell line for isolation of measles virus from clinical specimens and establishment of RT-PCR and automated DNA sequencing techniques have allowed for rapid genetic characterization of a large number of wild-type strains of measles virus. A database of sequence information now makes it possible to use molecular epidemiological techniques to identify the source of wild-type viruses and to differentiate between wild type and vaccine strains.
The Vero/hSLAM cell line [hSLAM=(human) signaling lymphocytic activation molecule] is recommended for use in the laboratories that provide measles surveillance as part of the World Health Organization's Measles and Rubella Laboratory Network (WHO LabNet).[1]
The Vero/hSLAM cells are Vero cells that have been stably transfected with a plasmid encoding the gene for the human (h) SLAM molecule. SLAM (signaling lymphocyte activation molecule) has been shown to be a receptor for both wild-type and laboratory-adapted strains of measles. The sensitivity of Vero/hSLAM cells for isolation of measles virus is equivalent to that of B95a cells. The advantage to the Vero/hSLAM cells is that, unlike the B95a cells, they are not persistently infected with virus. This provides a significant safety advantage for laboratorians and greatly facilitates international shipments. The disadvantage of the Vero/hSLAM cells is that they must be cultured in medium containing geneticin to retain SLAM expression. This increases the cost of the tissue culture medium.
Genetic Analysis of Measles Viruses
References
- ↑ Featherstone, D. A.; Rota, P. A.; Icenogle, J.; Mulders, M. N.; Jee, Y.; Ahmed, H.; Bispo de Filippis, A. M.; Ramamurty, N.; Gavrilin, E.; Byabamazima, C.; Dosseh, A.; Xu, W.; Komase, K.; Tashiro, M.; Brown, D.; Bellini, W. J.; Strebel, P. (2011). "Expansion of the Global Measles and Rubella Laboratory Network 2005-09". Journal of Infectious Diseases. 204 (Supplement 1): S491–S498. doi:10.1093/infdis/jir107. ISSN 0022-1899.