Cardiac amyloidosis cardiac biopsy: Difference between revisions
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m Raviteja Reddy Guddeti moved page Cardiac amyloidosis other diagnostic studies to Cardiac amyloidosis cardiac biopsy without leaving a redirect |
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[[Biopsy]] is a standard diagnostic test in the evaluation of cardiac amyloidosis. Biopsied tissue is stained with amyloid specific stains to quantify the extent of involvement.<ref name="pmid3341867">{{cite journal |author=Pellikka PA, Holmes DR, Edwards WD, Nishimura RA, Tajik AJ, Kyle RA |title=Endomyocardial biopsy in 30 patients with primary amyloidosis and suspected cardiac involvement |journal=[[Archives of Internal Medicine]] |volume=148 |issue=3 |pages=662–6 |year=1988 |month=March |pmid=3341867 |doi= |url=}}</ref><ref name="pmid15131552">{{cite journal |author=Ardehali H, Qasim A, Cappola T, ''et al.'' |title=Endomyocardial biopsy plays a role in diagnosing patients with unexplained cardiomyopathy |journal=[[American Heart Journal]] |volume=147 |issue=5 |pages=919–23 |year=2004 |month=May |pmid=15131552 |doi=10.1016/j.ahj.2003.09.020 |url=}}</ref><ref name="pmid55419">{{cite journal |author=Pomerance A, Slavin G, McWatt J |title=Experience with the sodium sulphate-Alcian Blue stain for amyloid in cardiac pathology |journal=[[Journal of Clinical Pathology]] |volume=29 |issue=1 |pages=22–6 |year=1976 |month=January |pmid=55419 |pmc=475937 |doi= |url=}}</ref> | [[Biopsy]] is a standard diagnostic test in the evaluation of cardiac amyloidosis. Biopsied tissue is stained with amyloid specific stains to quantify the extent of involvement.<ref name="pmid3341867">{{cite journal |author=Pellikka PA, Holmes DR, Edwards WD, Nishimura RA, Tajik AJ, Kyle RA |title=Endomyocardial biopsy in 30 patients with primary amyloidosis and suspected cardiac involvement |journal=[[Archives of Internal Medicine]] |volume=148 |issue=3 |pages=662–6 |year=1988 |month=March |pmid=3341867 |doi= |url=}}</ref><ref name="pmid15131552">{{cite journal |author=Ardehali H, Qasim A, Cappola T, ''et al.'' |title=Endomyocardial biopsy plays a role in diagnosing patients with unexplained cardiomyopathy |journal=[[American Heart Journal]] |volume=147 |issue=5 |pages=919–23 |year=2004 |month=May |pmid=15131552 |doi=10.1016/j.ahj.2003.09.020 |url=}}</ref><ref name="pmid55419">{{cite journal |author=Pomerance A, Slavin G, McWatt J |title=Experience with the sodium sulphate-Alcian Blue stain for amyloid in cardiac pathology |journal=[[Journal of Clinical Pathology]] |volume=29 |issue=1 |pages=22–6 |year=1976 |month=January |pmid=55419 |pmc=475937 |doi= |url=}}</ref> | ||
* [[Congo red]] staining of the specimen appears as an amorphous pink deposit under light microscopy and as green-apple birefringence under polarized microscopy. | * [[Congo red]] staining of the specimen appears as an amorphous pink deposit under light microscopy and as green-apple birefringence under polarized microscopy. | ||
* An alternative stain is | * An alternative stain is highly amyloid specific [[Alcian blue]], producing a green color on staining. | ||
* Immunostaining of the biopsy specimen is required to identify the deposited protein and to differentiate between ATTR and AL types of cardiac amyloidosis. | * Immunostaining of the biopsy specimen is required to identify the deposited protein and to differentiate between ATTR and AL types of cardiac amyloidosis. | ||
Revision as of 23:55, 4 May 2013
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Cardiac amyloidosis Microchapters |
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Cardiac amyloidosis cardiac biopsy On the Web |
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Risk calculators and risk factors for Cardiac amyloidosis cardiac biopsy |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Raviteja Guddeti, M.B.B.S. [2]; Aarti Narayan, M.B.B.S [3]
Overview
A cardiac biopsy that reveals amyloid confirms the diagnosis. Biopsy of other tissues may also confirm the diagnosis. Amyloidosis is frequently confirmed by biopsy of abdominal fat, rectal submucosa, kidney, or bone marrow.
Cardiac Biopsy
Biopsy is a standard diagnostic test in the evaluation of cardiac amyloidosis. Biopsied tissue is stained with amyloid specific stains to quantify the extent of involvement.[1][2][3]
- Congo red staining of the specimen appears as an amorphous pink deposit under light microscopy and as green-apple birefringence under polarized microscopy.
- An alternative stain is highly amyloid specific Alcian blue, producing a green color on staining.
- Immunostaining of the biopsy specimen is required to identify the deposited protein and to differentiate between ATTR and AL types of cardiac amyloidosis.
Advantages
- Most accurate with a sensitivity approaching 100%
- Safe in experienced hands
Dis-advantages
- Invasive
- Expensive
- Lack of availability
References
- ↑ Pellikka PA, Holmes DR, Edwards WD, Nishimura RA, Tajik AJ, Kyle RA (1988). "Endomyocardial biopsy in 30 patients with primary amyloidosis and suspected cardiac involvement". Archives of Internal Medicine. 148 (3): 662–6. PMID 3341867. Unknown parameter
|month=ignored (help) - ↑ Ardehali H, Qasim A, Cappola T; et al. (2004). "Endomyocardial biopsy plays a role in diagnosing patients with unexplained cardiomyopathy". American Heart Journal. 147 (5): 919–23. doi:10.1016/j.ahj.2003.09.020. PMID 15131552. Unknown parameter
|month=ignored (help) - ↑ Pomerance A, Slavin G, McWatt J (1976). "Experience with the sodium sulphate-Alcian Blue stain for amyloid in cardiac pathology". Journal of Clinical Pathology. 29 (1): 22–6. PMC 475937. PMID 55419. Unknown parameter
|month=ignored (help)