Mycobacterium abscessus causes: Difference between revisions
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Mycobacterium abscessus is a bacterium distantly related to the ones that cause [[tuberculosis]] and [[leprosy]]. It is part of a group known as rapidly growing mycobacteria and is found in water, soil, and dust. It has been known to contaminate medications and products, including medical devices. | Mycobacterium abscessus is a bacterium distantly related to the ones that cause [[tuberculosis]] and [[leprosy]]. It is part of a group known as rapidly growing mycobacteria and is found in water, soil, and dust. It has been known to contaminate medications and products, including medical devices. | ||
==Taxonomy== | |||
* Name: ''Mycobacterium abscessus'' | * Name: ''Mycobacterium abscessus'' | ||
* Regnum: [[Bacterium|Bacteria]] | * Regnum: [[Bacterium|Bacteria]] | ||
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* Binomial: ''Mycobacterium abscessus'' | * Binomial: ''Mycobacterium abscessus'' | ||
==Transmission== | |||
Infection with M. abscessus is usually caused by injections of substances contaminated with the bacterium or through invasive medical procedures employing contaminated equipment or material. Infection can also occur after accidental injury where the wound is contaminated by soil. There is very little risk of transmission from person to person. | Infection with M. abscessus is usually caused by injections of substances contaminated with the bacterium or through invasive medical procedures employing contaminated equipment or material. Infection can also occur after accidental injury where the wound is contaminated by soil. There is very little risk of transmission from person to person. | ||
Mycobacterium abscessus is relatively resistant to [[chlorine]] and standard desinfectant.<ref name="pmid9891805">{{cite journal| author=Wallace RJ, Brown BA, Griffith DE| title=Nosocomial outbreaks/pseudo-outbreaks caused by nontuberculous mycobacteria. | journal=Annu Rev Microbiol | year= 1998 | volume= 52 | issue= | pages= 453-90 | pmid=9891805 | doi=10.1146/annurev.micro.52.1.453 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=9891805 }} </ref> | Mycobacterium abscessus is relatively resistant to [[chlorine]] and standard desinfectant.<ref name="pmid9891805">{{cite journal| author=Wallace RJ, Brown BA, Griffith DE| title=Nosocomial outbreaks/pseudo-outbreaks caused by nontuberculous mycobacteria. | journal=Annu Rev Microbiol | year= 1998 | volume= 52 | issue= | pages= 453-90 | pmid=9891805 | doi=10.1146/annurev.micro.52.1.453 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=9891805 }} </ref> | ||
==Microscopy== | |||
*Gram-positive, nonmotile and acid-fast rods (1.0-2.5µm x 0.5µm). | *Gram-positive, nonmotile and acid-fast rods (1.0-2.5µm x 0.5µm). | ||
==Colony characteristics== | |||
*Colonies on Löwenstein-Jensen media may occur as smooth as well as rough, white or greyish and nonphotochromogenic. | *Colonies on Löwenstein-Jensen media may occur as smooth as well as rough, white or greyish and nonphotochromogenic. | ||
==Physiology== | |||
*Growth at 28°C and 37°C after 7 days but not at 43°C. | *Growth at 28°C and 37°C after 7 days but not at 43°C. | ||
*On MacConkey agar at 28°C and even 37°C. | *On MacConkey agar at 28°C and even 37°C. | ||
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*Negative iron uptake test. No utilisation of fructose, glucose, oxalate and citrate as sole carbon sources. | *Negative iron uptake test. No utilisation of fructose, glucose, oxalate and citrate as sole carbon sources. | ||
==Differential Characteristics== | |||
*M. abscessus and M. chelonae can be distinguished from M. fortuitum or M. peregrinum by their failure to reduce nitrate and to take up iron. | *M. abscessus and M. chelonae can be distinguished from M. fortuitum or M. peregrinum by their failure to reduce nitrate and to take up iron. | ||
*Tolerance to 5% NaCl in Löwenstein-Jensen media tolerance to 0.2% picrate in Sauton agar and non-utilisation of citrate as a sole carbon source are characteristics that distinguish M. abscessus from M. chelonae. | *Tolerance to 5% NaCl in Löwenstein-Jensen media tolerance to 0.2% picrate in Sauton agar and non-utilisation of citrate as a sole carbon source are characteristics that distinguish M. abscessus from M. chelonae. | ||
*M. abscessus and M. chelonae sequevar I share an identical sequence in the 54-510 region of 16S rRNA, However, both species can be differentiated by their hsp65 or ITS sequences | *M. abscessus and M. chelonae sequevar I share an identical sequence in the 54-510 region of 16S rRNA, However, both species can be differentiated by their hsp65 or ITS sequences | ||
==Strains== | |||
ATCC 19977 = CCUG 20993 = CIP 104536 = DSM 44196 = JCM 13569 = NCTC 13031 | ATCC 19977 = CCUG 20993 = CIP 104536 = DSM 44196 = JCM 13569 = NCTC 13031 | ||
==Genetics== | |||
A draft genome sequence of ''M. abscessus'' subsp. ''bolletii'' BD<sup>T</sup> was completed in 2012.<ref>{{cite journal|last=Choi|first=G.-E.|author2=Cho, Y.-J. |author3=Koh, W.-J. |author4=Chun, J. |author5=Cho, S.-N. |author6= Shin, S. J. |title=Draft Genome Sequence of Mycobacterium abscessus subsp. bolletii BDT|journal=Journal of Bacteriology|date=24 April 2012|volume=194|issue=10|pages=2756–2757|doi=10.1128/JB.00354-12}}</ref> More than 25 different strains of this subspecies, including pathogenic isolates, have had their genomes sequenced.<ref>{{cite journal|last=Davidson|first=Rebecca M.|coauthors=Hasan, Nabeeh A.; de Moura, Vinicius Calado Nogueira; Duarte, Rafael Silva; Jackson, Mary; Strong, Michael|title=Phylogenomics of Brazilian epidemic isolates of Mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains|journal=Infection, Genetics and Evolution|date=December 2013|volume=20|pages=292–297|doi=10.1016/j.meegid.2013.09.012}}</ref> | A draft genome sequence of ''M. abscessus'' subsp. ''bolletii'' BD<sup>T</sup> was completed in 2012.<ref>{{cite journal|last=Choi|first=G.-E.|author2=Cho, Y.-J. |author3=Koh, W.-J. |author4=Chun, J. |author5=Cho, S.-N. |author6= Shin, S. J. |title=Draft Genome Sequence of Mycobacterium abscessus subsp. bolletii BDT|journal=Journal of Bacteriology|date=24 April 2012|volume=194|issue=10|pages=2756–2757|doi=10.1128/JB.00354-12}}</ref> More than 25 different strains of this subspecies, including pathogenic isolates, have had their genomes sequenced.<ref>{{cite journal|last=Davidson|first=Rebecca M.|coauthors=Hasan, Nabeeh A.; de Moura, Vinicius Calado Nogueira; Duarte, Rafael Silva; Jackson, Mary; Strong, Michael|title=Phylogenomics of Brazilian epidemic isolates of Mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains|journal=Infection, Genetics and Evolution|date=December 2013|volume=20|pages=292–297|doi=10.1016/j.meegid.2013.09.012}}</ref> | ||
==Resistance to Antibiotics== | |||
===Intrinsic Factors=== | |||
* The permeability barrier of the envelope of the myobacterium<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | * The permeability barrier of the envelope of the myobacterium<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | ||
* Low affinity of the antibiotics to their target<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | * Low affinity of the antibiotics to their target<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | ||
| Line 55: | Line 54: | ||
* Neutralization of the antibiotics by cytoplasmic enzymes<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | * Neutralization of the antibiotics by cytoplasmic enzymes<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | ||
===Acquired Factors=== | |||
* Mutation of the genes that code the antibiotic targets<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | * Mutation of the genes that code the antibiotic targets<ref name="pmid22290346">{{cite journal| author=Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B| title=Mycobacterium abscessus: a new antibiotic nightmare. | journal=J Antimicrob Chemother | year= 2012 | volume= 67 | issue= 4 | pages= 810-8 | pmid=22290346 | doi=10.1093/jac/dkr578 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=22290346 }} </ref> | ||
Revision as of 23:29, 23 July 2014
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Rim Halaby, M.D. [2]
Overview
Mycobacterium abscessus is a bacterium distantly related to the ones that cause tuberculosis and leprosy. It is part of a group known as rapidly growing mycobacteria and is found in water, soil, and dust. It has been known to contaminate medications and products, including medical devices.
Taxonomy
- Name: Mycobacterium abscessus
- Regnum: Bacteria
- Phylum: Actinobacteria
- Ordo: Actinomycetales
- Subordo: Corynebacterineae
- Familia: Mycobacteriaceae
- Genus: Mycobacterium
- Species: M. abscessus
- Binomial: Mycobacterium abscessus
Transmission
Infection with M. abscessus is usually caused by injections of substances contaminated with the bacterium or through invasive medical procedures employing contaminated equipment or material. Infection can also occur after accidental injury where the wound is contaminated by soil. There is very little risk of transmission from person to person.
Mycobacterium abscessus is relatively resistant to chlorine and standard desinfectant.[1]
Microscopy
- Gram-positive, nonmotile and acid-fast rods (1.0-2.5µm x 0.5µm).
Colony characteristics
- Colonies on Löwenstein-Jensen media may occur as smooth as well as rough, white or greyish and nonphotochromogenic.
Physiology
- Growth at 28°C and 37°C after 7 days but not at 43°C.
- On MacConkey agar at 28°C and even 37°C.
- Tolerance to 5% NaCl and 500mg/l hydroxylamine (Ogawa egg medium) and 0.2% picrate (Sauton agar medium).
- Positive degradation of p-aminosalicylate.
- Production of arylsulfatase but not of nitrate reductase and Tween 80 hydrolase.
- Negative iron uptake test. No utilisation of fructose, glucose, oxalate and citrate as sole carbon sources.
Differential Characteristics
- M. abscessus and M. chelonae can be distinguished from M. fortuitum or M. peregrinum by their failure to reduce nitrate and to take up iron.
- Tolerance to 5% NaCl in Löwenstein-Jensen media tolerance to 0.2% picrate in Sauton agar and non-utilisation of citrate as a sole carbon source are characteristics that distinguish M. abscessus from M. chelonae.
- M. abscessus and M. chelonae sequevar I share an identical sequence in the 54-510 region of 16S rRNA, However, both species can be differentiated by their hsp65 or ITS sequences
Strains
ATCC 19977 = CCUG 20993 = CIP 104536 = DSM 44196 = JCM 13569 = NCTC 13031
Genetics
A draft genome sequence of M. abscessus subsp. bolletii BDT was completed in 2012.[2] More than 25 different strains of this subspecies, including pathogenic isolates, have had their genomes sequenced.[3]
Resistance to Antibiotics
Intrinsic Factors
- The permeability barrier of the envelope of the myobacterium[4]
- Low affinity of the antibiotics to their target[4]
- Drug export systems[4]
- Neutralization of the antibiotics by cytoplasmic enzymes[4]
Acquired Factors
- Mutation of the genes that code the antibiotic targets[4]
References
- ↑ Wallace RJ, Brown BA, Griffith DE (1998). "Nosocomial outbreaks/pseudo-outbreaks caused by nontuberculous mycobacteria". Annu Rev Microbiol. 52: 453–90. doi:10.1146/annurev.micro.52.1.453. PMID 9891805.
- ↑ Choi, G.-E.; Cho, Y.-J.; Koh, W.-J.; Chun, J.; Cho, S.-N.; Shin, S. J. (24 April 2012). "Draft Genome Sequence of Mycobacterium abscessus subsp. bolletii BDT". Journal of Bacteriology. 194 (10): 2756–2757. doi:10.1128/JB.00354-12.
- ↑ Davidson, Rebecca M. (December 2013). "Phylogenomics of Brazilian epidemic isolates of Mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains". Infection, Genetics and Evolution. 20: 292–297. doi:10.1016/j.meegid.2013.09.012. Unknown parameter
|coauthors=ignored (help) - ↑ 4.0 4.1 4.2 4.3 4.4 Nessar R, Cambau E, Reyrat JM, Murray A, Gicquel B (2012). "Mycobacterium abscessus: a new antibiotic nightmare". J Antimicrob Chemother. 67 (4): 810–8. doi:10.1093/jac/dkr578. PMID 22290346.