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{{Influenza}}
{{Influenza}}
{{CMG}}; {{AE}} {{AL}}
==Overview==
==Other Diagnostic Studies==
===Molecular Assays<small><small><small> Adapted from CDC <ref name=CDC> {{cite web|url=http://www.cdc.gov/flu/professionals/diagnosis/molecular-assays.htm| title=CDC Guidance for Clinicians on the Use of RT-PCR and Other Molecular Assays for Diagnosis of Influenza Virus Infection}} </ref> </small></small></small>===
*Tests for influenza include molecular assays, rapid influenza diagnostic tests, viral culture or serology.
*Molecular assays for influenza are increasingly being used in clinical settings.
*Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and other molecular assays can identify the presence of influenza viral RNA in respiratory specimens.
*RT-PCR is particularly useful to identify influenza virus infection as a cause of respiratory outbreaks in institutions, such as nursing homes, chronic care facilities, and hospitals.
*Some molecular assays are able to detect and discriminate between infections with influenza A and B viruses; other tests can identify specific influenza A virus subtypes [A(H1N1)pdm09, seasonal A (H1N1), or seasonal A (H3N2)].
*These assays can yield results in 3-8 hours.
*Notably, the detection of influenza viral RNA by these assays does not always indicate detection of viable virus or on-going influenza viral replication.
*It is important to note that not all assays have been cleared by the FDA for diagnostic use.


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==References==
==References==
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[[Category:Infectious disease]]
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Revision as of 13:04, 28 October 2014

Influenza Microchapters

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Alejandro Lemor, M.D. [2]

Overview

Other Diagnostic Studies

Molecular Assays Adapted from CDC [1]

  • Tests for influenza include molecular assays, rapid influenza diagnostic tests, viral culture or serology.
  • Molecular assays for influenza are increasingly being used in clinical settings.
  • Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and other molecular assays can identify the presence of influenza viral RNA in respiratory specimens.
  • RT-PCR is particularly useful to identify influenza virus infection as a cause of respiratory outbreaks in institutions, such as nursing homes, chronic care facilities, and hospitals.
  • Some molecular assays are able to detect and discriminate between infections with influenza A and B viruses; other tests can identify specific influenza A virus subtypes [A(H1N1)pdm09, seasonal A (H1N1), or seasonal A (H3N2)].
  • These assays can yield results in 3-8 hours.
  • Notably, the detection of influenza viral RNA by these assays does not always indicate detection of viable virus or on-going influenza viral replication.
  • It is important to note that not all assays have been cleared by the FDA for diagnostic use.



References

  1. "CDC Guidance for Clinicians on the Use of RT-PCR and Other Molecular Assays for Diagnosis of Influenza Virus Infection".

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