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*Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and other molecular assays can identify the presence of influenza viral RNA in respiratory specimens. | *Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and other molecular assays can identify the presence of influenza viral RNA in respiratory specimens. | ||
*RT-PCR is particularly useful to identify influenza virus infection as a cause of respiratory outbreaks in institutions, such as nursing homes, chronic care facilities, and hospitals. | *RT-PCR is particularly useful to identify influenza virus infection as a cause of respiratory outbreaks in institutions, such as nursing homes, chronic care facilities, and hospitals. | ||
*Some molecular assays are able to detect and discriminate between infections with influenza A and B viruses; other tests can identify specific influenza A virus subtypes [A(H1N1)pdm09, seasonal A (H1N1), or seasonal A (H3N2)]. | *Some molecular assays are able to detect and discriminate between infections with influenza A and B viruses; other tests can identify specific influenza A virus subtypes [A(H1N1)pdm09, seasonal A (H1N1), or seasonal A (H3N2)]. | ||
*These assays can yield results in 3-8 hours. | *These assays can yield results in 3-8 hours. | ||
*Notably, the detection of influenza viral RNA by these assays does not always indicate detection of viable virus or on-going influenza viral replication. | *Notably, the detection of influenza viral RNA by these assays does not always indicate detection of viable virus or on-going influenza viral replication. | ||
*It is important to note that not all assays have been cleared by the FDA for diagnostic use. | *It is important to note that not all assays have been cleared by the FDA for diagnostic use. | ||
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==References== | ==References== | ||
Revision as of 13:06, 28 October 2014
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Alejandro Lemor, M.D. [2]
Overview
Other Diagnostic Studies
Molecular Assays Adapted from CDC [1]
- Tests for influenza include molecular assays, rapid influenza diagnostic tests, viral culture or serology.
- Molecular assays for influenza are increasingly being used in clinical settings.
- Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and other molecular assays can identify the presence of influenza viral RNA in respiratory specimens.
- RT-PCR is particularly useful to identify influenza virus infection as a cause of respiratory outbreaks in institutions, such as nursing homes, chronic care facilities, and hospitals.
- Some molecular assays are able to detect and discriminate between infections with influenza A and B viruses; other tests can identify specific influenza A virus subtypes [A(H1N1)pdm09, seasonal A (H1N1), or seasonal A (H3N2)].
- These assays can yield results in 3-8 hours.
- Notably, the detection of influenza viral RNA by these assays does not always indicate detection of viable virus or on-going influenza viral replication.
- It is important to note that not all assays have been cleared by the FDA for diagnostic use.