Acute myeloid leukemia other diagnostic studies: Difference between revisions

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==Other Diagnostic Studies==
==Other Diagnostic Studies==
===Fluorescent in situ hybridization===
Because [[acute promyelocytic leukemia]] (APL) has the highest curability and requires a unique form of treatment, it is important to quickly establish or exclude the diagnosis of this subtype of leukemia.  [[Fluorescent in situ hybridization]] performed on blood or bone marrow is often used for this purpose, as it readily identifies the [[chromosomal translocation]] (t&#91;15;17&#93;) that characterizes APL.<ref>{{cite journal | author = Grimwade D, Howe K, Langabeer S, Davies L, Oliver F, Walker H, Swirsky D, Wheatley K, Goldstone A, Burnett A, Solomon E | title = Establishing the presence of the t(15;17) in suspected acute promyelocytic leukaemia: cytogenetic, molecular and PML immunofluorescence assessment of patients entered into the M.R.C. ATRA trial. M.R.C. Adult Leukaemia Working Party. | journal = Br J Haematol | volume = 94 | issue = 3 | pages = 557-73 | year = 1996 | pmid = 8790159}}</ref>
===Cytochemistry===
===Cytochemistry===
This is a test used to differentiate acute myeloid leukemia from acute lymphoblastic leukemia using a stain which reacts to a particular type of leukemic cells.  The stain causes the granules of most acute myeloid leukemia cells to appear as black spots under the microscope, but it does not cause ALL cells to change colors.
This is a test used to differentiate acute myeloid leukemia from acute lymphoblastic leukemia. Using a stain which reacts to a particular type of leukemic cells.  The stain causes the granules of most acute myeloid leukemia cells to appear as black spots under the microscope, but it does not cause Acute lymphoblastic cells to change colors.


===Flow cytometry and Immunohistochemistry===
===Flow cytometry and Immunohistochemistry===
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===PCR===
===PCR===
PCR is a highly sensitive DNA test used to recognize cancer cells.
PCR is a highly sensitive DNA test used to recognize cancer cells in acute myeloid leukemia.


==References==
==References==

Revision as of 15:48, 2 September 2015

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Raviteja Guddeti, M.B.B.S. [2] Carlos A Lopez, M.D. [3]

Overview

Other Diagnostic Studies

Cytochemistry

This is a test used to differentiate acute myeloid leukemia from acute lymphoblastic leukemia. Using a stain which reacts to a particular type of leukemic cells. The stain causes the granules of most acute myeloid leukemia cells to appear as black spots under the microscope, but it does not cause Acute lymphoblastic cells to change colors.

Flow cytometry and Immunohistochemistry

In these tests special antibodies are used to detect cancer cells and thus to differentiate acute myeloid leukemia from other leukemias.

PCR

PCR is a highly sensitive DNA test used to recognize cancer cells in acute myeloid leukemia.

References

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