Norovirus infection laboratory findings

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Overview

Laboratory findings of norovirus infection include elevated concentration of inflammatory markers, hypokalemia, and chloride-sensitive metabolic alkalosis. Signs of dehydration may be present, such as relative polycythemia, elevated BUN, and elevated creatinine (pre-renal acute kidney injury). RT-qPCR assay is the optimal test for the diagnosis of norovirus infection. Enzyme immunoassays to detect norovirus have been developed but are less useful due to low sensitivity. Norovirus is not usually grown on culture.

Laboratory Findings

• Lab findings of norovirus infection are usually related to the degree of dehydration. Lab findings include:

• WBC count may be normal or elevated

• Elevated concentration of inflammatory markers (e.g. CRP or ESR)

• Chloride-sensitive metabolic alkalosis and electrolyte derangements (commmonly hypokalemia)

• Relative polycythemia

• Elevated concentration of BUN

• Elevated creatinine is suggestive of pre-renal acute kidney injury in severe dehydration

Diagnostic Methods

RT-qPCR Assays

• RT-qPCR assays are the preferred laboratory method for detecting norovirus.
• These assays are very sensitive and can detect as few as 10 to 100 norovirus copies per reaction.*They use different primers to differentiate genogroup I and genogroup II norovirus.
• RT-qPCR assays are also quantitative and can provide estimates of viral load.
• The assays may be used to detect norovirus in stool, vomitus, foods, water, and environmental specimens.

Conventional RT-PCR Assays for Genotyping

• Conventional RT-PCR followed by sequence analysis of the RT-PCR products is used for norovirus genotyping.
• Typically, a partial region of the capsid gene, such as region D, is used by laboratories participating in CaliciNet, a national laboratory surveillance network for norovirus outbreaks coordinated by CDC.

Enzyme Immunoassays

• Rapid commercial assays, such as enzyme immunoassays (EIAs), that detect norovirus antigen have recently been developed.

• However, these kits have poor sensitivity (50%) and are not recommended for diagnosing norovirus infection in sporadic cases of gastroenteritis.
• The RIDASCREEN Norovirus 3rd Generation EIA was recently cleared by Food and Drug Administration for preliminary identification of norovirus when testing multiple specimens during outbreaks.
• Samples that test negative should be confirmed by a second technique, such as RT-qPCR.
• EIA kits should not replace molecular methods during outbreak investigations.

Culture

• Human noroviruses cannot be grown in cell culture.
• Diagnostic methods focus on detecting viral RNA or antigen.

Specimen Collection

Clinical Specimens

• Stool: Whole stool is the preferred clinical specimen for laboratory diagnosis of norovirus. Ideally, specimens should be collected during the acute phase of illness (within 48 to 72 hours after symptoms start) while stools are still liquid or semisolid. Virus is excreted in the greatest amount during this time. Norovirus can sometimes be detected in stool specimens that are collected later in the illness or after the symptoms have resolved (up to 7 to 10 days after onset). Whole stool specimens should be kept refrigerated at 39°F (4°C) if testing is done within 2 to 3 weeks. If the specimens are shipped to a laboratory for testing, each sample should be sealed in a separate bag, and kept on frozen refrigerant packs in an insulated, waterproof polystyrene container. If testing will be done more than 3 weeks after the specimens are collected, they should be frozen at -4°F (-20°C) or -94°F (-70°C). When the specimens are stored in this way, norovirus can be detected after at least 5 years.
• Vomitus: Vomitus can be collected to supplement stool specimens during an investigation. These specimens should be collected, stored, and shipped in the same way as stool specimens.
• Serum: Serum specimens are not recommended for routine laboratory diagnosis of norovirus. If feasible and warranted for special studies, acute- and convalescent-phase serum specimens may be collected and tested for a greater than fourfold rise in IgG titer to noroviruses. Acute-phase serum specimens should be collected during the first 5 days after the symptoms start. Convalescent-phase specimens should be collected during the third to fourth week after the symptoms start.

Food, Water, and Environmental Specimens

• Food and Water Specimens: In principle, norovirus can be detected in water, food, and environmental specimens. However, the virus first needs to be concentrated or extracted or both from the specimen. Validated methods for these techniques are available only for water (at CDC) and shellfish [at the Gulf Coast Seafood Laboratory, Food and Drug Administration (FDA)]. If food or water is the suspected cause of a norovirus outbreak, samples should be collected as soon as possible after people were exposed. Food specimens should be stored frozen at -4°F (-20°C). Water can be tested for norovirus by processing large volumes (up to 100L) through specially designed filters. Water samples should be stored refrigerated or chilled on ice at 39°F (4°C).
• Environmental Specimens: Norovirus RNA has been detected in swabs of environmental surfaces collected in specific outbreak settings. However, obtaining virus from swabs is highly variable. Results should be interpreted with caution and in the context of the available epidemiologic evidence.

References

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