Cryptococcosis laboratory tests: Difference between revisions
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==Laboratory Findings== | ==Laboratory Findings== | ||
Detection of cryptococcal [[antigen]] (capsular material) by [[Microbiological culture|culture]] of [[Cerebrospinal fluid|CSF]], [[sputum]] and [[urine]] provides definitive diagnosis. | |||
Detection of cryptococcal [[antigen]] (capsular material) by [[Microbiological culture|culture]] of [[Cerebrospinal fluid|CSF]], [[sputum]] and [[urine]] provides definitive diagnosis. Blood cultures may be positive in heavy infections. | ====Culture==== | ||
*The gold standard for diagnosing cryptococcal infection; culture is traditionally identify Cryptococcus from human body samples. | |||
*Blood cultures may be positive in heavy infections. | |||
* | ====Microscopy==== | ||
* | *India Ink can be performed on CSF to quickly visualize Cryptococcus cells under a microscope; however, it can have limited sensitivity. | ||
* | *Histopathology for detection of narrow-based budding yeasts in tissue can also be used. | ||
* | ====Antigen detection==== | ||
* Antigen detection can be used on CSF or serum for detection of early, asymptomatic cryptococcal infection in HIV-infected patients. | |||
* | * It has a higher sensitivity than microscopy or culture. | ||
* | *Techniques used include: | ||
* | :*Latex agglutination (LA) | ||
:*Enzyme immunoassay (EIA) | |||
:*Lateral flow assay (LFA) | |||
==References== | ==References== | ||
Revision as of 16:08, 31 December 2015
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
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Cryptococcosis Microchapters |
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Diagnosis |
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Cryptococcosis laboratory tests On the Web |
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American Roentgen Ray Society Images of Cryptococcosis laboratory tests |
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Risk calculators and risk factors for Cryptococcosis laboratory tests |
Overview
Laboratory Findings
Detection of cryptococcal antigen (capsular material) by culture of CSF, sputum and urine provides definitive diagnosis.
Culture
- The gold standard for diagnosing cryptococcal infection; culture is traditionally identify Cryptococcus from human body samples.
- Blood cultures may be positive in heavy infections.
Microscopy
- India Ink can be performed on CSF to quickly visualize Cryptococcus cells under a microscope; however, it can have limited sensitivity.
- Histopathology for detection of narrow-based budding yeasts in tissue can also be used.
Antigen detection
- Antigen detection can be used on CSF or serum for detection of early, asymptomatic cryptococcal infection in HIV-infected patients.
- It has a higher sensitivity than microscopy or culture.
- Techniques used include:
- Latex agglutination (LA)
- Enzyme immunoassay (EIA)
- Lateral flow assay (LFA)