Trichomoniasis laboratory findings: Difference between revisions
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Trichomoniasis is diagnosed by visually observing the trichomonads via a [[microscope]]. In women, the examiner collects the specimen during a [[pelvic examination]] by inserting a [[Speculum (medical)|speculum]] into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor [[Sensitivity|sensitivity]] of the tests.<ref name=Andrea&Chapin2011>{{cite journal| author=Andrea SB, Chapin KC| title=Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications. | journal=J Clin Microbiol | year= 2011 | volume= 49 | issue= 3 | pages= 866–9 | pmid=21248097 | doi=10.1128/JCM.02367-10 | pmc= | url= | laysummary = http://www.eurekalert.org/pub_releases/2011-03/l-amd030811.php}}</ref> | Trichomoniasis is diagnosed by visually observing the trichomonads via a [[microscope]]. In women, the examiner collects the specimen during a [[pelvic examination]] by inserting a [[Speculum (medical)|speculum]] into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor [[Sensitivity|sensitivity]] of the tests.<ref name=Andrea&Chapin2011>{{cite journal| author=Andrea SB, Chapin KC| title=Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications. | journal=J Clin Microbiol | year= 2011 | volume= 49 | issue= 3 | pages= 866–9 | pmid=21248097 | doi=10.1128/JCM.02367-10 | pmc= | url= | laysummary = http://www.eurekalert.org/pub_releases/2011-03/l-amd030811.php}}</ref> | ||
Findings on microscopy suggestive of trichomoniasis include:<ref name="pmid23633669">{{cite journal| author=Hobbs MM, Seña AC| title=Modern diagnosis of Trichomonas vaginalis infection. | journal=Sex Transm Infect | year= 2013 | volume= 89 | issue= 6 | pages= 434-8 | pmid=23633669 | doi=10.1136/sextrans-2013-051057 | pmc=PMC3787709 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=23633669 }} </ref><ref name="pmid15489349">{{cite journal| author=Schwebke JR, Burgess D| title=Trichomoniasis. | journal=Clin Microbiol Rev | year= 2004 | volume= 17 | issue= 4 | pages= 794-803, table of contents | pmid=15489349 | doi=10.1128/CMR.17.4.794-803.2004 | pmc=PMC523559 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=15489349 }} </ref> | |||
*Characteristic "tumbling" motility of protozoa | |||
*[[Leukocytes]] | |||
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Revision as of 22:31, 4 February 2016
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Trichomoniasis Microchapters |
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Maliha Shakil, M.D. [2]
Overview
Laboratory tests used in the diagnosis of trichomoniasis include saline microscopy, culture, and nucleic acid amplification tests (NAATs).[1]
Laboratory Findings
Microscopy
Trichomoniasis is diagnosed by visually observing the trichomonads via a microscope. In women, the examiner collects the specimen during a pelvic examination by inserting a speculum into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor sensitivity of the tests.[2]
Findings on microscopy suggestive of trichomoniasis include:[3][4]
- Characteristic "tumbling" motility of protozoa
- Leukocytes
| Trichomonas vaginalis | Pap smear |
|---|---|
 |
|
Two trophozoites of Trichomonas vaginalis obtained from in vitro culture. Smear was stained with Giemsa.[5]
Culture
Nucleic Acid Amplification Tests
References
- ↑ Trichomonasias . Wikipedia.https://en.wikipedia.org/wiki/Trichomoniasis Accessed on February 4, 2016
- ↑ Andrea SB, Chapin KC (2011). "Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications". J Clin Microbiol. 49 (3): 866–9. doi:10.1128/JCM.02367-10. PMID 21248097. Lay summary.
- ↑ Hobbs MM, Seña AC (2013). "Modern diagnosis of Trichomonas vaginalis infection". Sex Transm Infect. 89 (6): 434–8. doi:10.1136/sextrans-2013-051057. PMC 3787709. PMID 23633669.
- ↑ Schwebke JR, Burgess D (2004). "Trichomoniasis". Clin Microbiol Rev. 17 (4): 794–803, table of contents. doi:10.1128/CMR.17.4.794-803.2004. PMC 523559. PMID 15489349.
- ↑ "DPDx - Trichomoniasis". Retrieved 2012-12-27.