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| ==Purpose==
| | #REDIRECT [[Tick-borne encephalitis]] |
| *TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.
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| ==Recipe==
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| '''10x TBE (1 liter):'''
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| #Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water. | |
| #Add 40 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0) (alternatively use 9.3 g Na<sub>2</sub>EDTA)
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| #Adjust volume to 1 Liter.
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| #Store at room temperature.
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| '''Note:''' 10x TBE may take some time to dissolve, even with fast stirring
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| '''1x TBE (1 liter):'''
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| #Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
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| #Add 4 ml 0.5 M Na<sub>2</sub>EDTA (pH 8.0)
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| #Adjust volume to 1 Liter.
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| #Store at room temperature.
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| ==Commercial sources==
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| #[https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=195&catname=North%20America%20Main UltraPure™ 10X TBE Buffer] from Invitrogen
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| [[Category:Material]] [[Category:Agarose gel electrophoresis]][[Category:Buffers]]
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