Rotavirus infection laboratory findings: Difference between revisions
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==Laboratory Findings== | ==Laboratory Findings== | ||
*Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease). | *Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue= | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214 }} </ref> | ||
Other diagnostic techniques that can be used: | Other diagnostic techniques that can be used: | ||
**Electron microscopy | **Electron microscopy | ||
Revision as of 15:20, 10 May 2017
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Ahmed Elsaiey, MBBCH [2]
Overview
Laboratory Findings
- Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).[1]
Other diagnostic techniques that can be used:
- Electron microscopy
- RNA electrophoresis
- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
- Tissue culture
- The virus is usually excreted in the stool in the first phases of the infection so, stool examination is required to detect the virus particles. Polyacrylamid gel electrophoresis can be used to detect the virus in the patients stool.
- Antigen detecting tests can be used to diagnose the rotavirus infection. It includes:
- Enzyme Linked Immunosorbent assay (ELISA)
- Immunochromatographic assay
References
- ↑ Parashar UD, Nelson EA, Kang G (2013). "Diagnosis, management, and prevention of rotavirus gastroenteritis in children". BMJ. 347: f7204. doi:10.1136/bmj.f7204. PMID 24379214.