Legionella pneumophila: Difference between revisions
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==Overview== | ==Overview== | ||
''L.pneumophila'' is a ubiquitous aquatic organism that thrives in warm environments (32°- 45°C). ''L. pneumophila'' is a pleomorphic, aerobic, catalase-positive, oxidase-positive, [[Endospore|non-spore-forming]], non-capsulated, motile, Gram-negative bacteria. Although ''Legionella'' is categorized as a Gram-negative bacterium, it stains poorly to Gram stain due to its unique [[lipopolysaccharide]]-content in the outer psuedospamodulating leaflet of the outer cell membrane. | ''L.pneumophila'' is a ubiquitous aquatic organism that thrives in warm environments (32°- 45°C). ''L. pneumophila'' is a [[pleomorphic]], [[aerobic]], [[catalase]]-positive, [[oxidase]]-positive, [[Endospore|non-spore-forming]], non-capsulated, [[motile]], Gram-negative bacteria. Although ''[[Legionella]]'' is categorized as a [[Gram-negative bacteria|Gram-negative bacterium]], it stains poorly to Gram stain due to its unique [[lipopolysaccharide]]-content in the outer psuedospamodulating leaflet of the outer cell membrane. | ||
==Microbiological Classification== | ==Microbiological Classification== | ||
*Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae | *[[Bacteria]]; [[Proteobacteria]]; [[Gammaproteobacteria]]; [[Legionellales]]; [[Legionellaceae]] | ||
==Microbiological Characteristics== | ==Microbiological Characteristics== | ||
*''L.pneumophila'' is a ubiquitous aquatic organism that thrives in warm environments (32°- 45°C). | *''L.pneumophila'' is a ubiquitous aquatic organism that thrives in warm environments (32°- 45°C). | ||
*''L. pneumophila'' is a pleomorphic, aerobic, catalase-positive, oxidase-positive, [[Endospore|non-spore-forming]], non-capsulated, motile, Gram-negative bacteria. | *''L. pneumophila'' is a pleomorphic, [[aerobic]], [[catalase]]-positive, [[oxidase]]-positive, [[Endospore|non-spore-forming]], non-capsulated, [[motile]], [[Gram-negative bacteria]]. | ||
*Although ''Legionella'' is categorized as a Gram-negative bacterium, it stains poorly to Gram stain due to its unique [[lipopolysaccharide]]-content in the outer psuedospamodulating leaflet of the outer cell membrane.<ref name=Sherris>{{cite book | author = Ryan KJ; Ray CG (editors) | title = Sherris Medical Microbiology | edition = 4th ed. | publisher = McGraw Hill | year = 2004 | id = ISBN 0-8385-8529-9 }}</ref> | *Although ''Legionella'' is categorized as a [[Gram-negative bacteria|Gram-negative bacterium]], it stains poorly to Gram stain due to its unique [[lipopolysaccharide]]-content in the outer psuedospamodulating leaflet of the outer cell membrane.<ref name="Sherris">{{cite book | author = Ryan KJ; Ray CG (editors) | title = Sherris Medical Microbiology | edition = 4th ed. | publisher = McGraw Hill | year = 2004 | id = ISBN 0-8385-8529-9 }}</ref> | ||
*''Legionella'' is non-fermentative and is unable to [[hydrolysis|hydrolyse]] [[gelatin]] or produce [[urease]]. | *''[[Legionella]]'' is non-fermentative and is unable to [[hydrolysis|hydrolyse]] [[gelatin]] or produce [[urease]]. | ||
*At least 46 species and 70 serogroups of ''Legionella'' have been identified. | *At least 46 species and 70 serogroups of ''[[Legionella]]'' have been identified. | ||
==Life Cycle== | ==Life Cycle== | ||
*There are two major phases to the life cycle. | *There are two major phases to the life cycle. | ||
:*The first is called the replicative phase. During this period, the bacteria are nonmotile and have a low toxicity. | :*The first is called the replicative phase. During this period, the [[bacteria]] are nonmotile and have a low toxicity. | ||
:*In the second phase, called the infectious phase, the bacteria are shorter and thicker. | :*In the second phase, called the infectious phase, the bacteria are shorter and thicker. | ||
==Gallery== | ==Gallery== | ||
[[File:Legionella microscopy.jpg|thumb|none|Colorized scanning electron micrograph (SEM) depicted a large grouping of Gram-negative Legionella pneumophila bacteria under 8000x magnification.<sup>Courtesy of Janice Haney Carr, Public Health Image Library<ref name=PHIL1>{{cite web |url=http://phil.cdc.gov/phil/details.asp |title=Public Health Image Library |last1=Haney Carr |first1=Janice |website= www.cdc.gov |access-date= Jan 15 2016}}</ref></sup>]] | [[File:Legionella microscopy.jpg|thumb|none|Colorized scanning electron micrograph (SEM) depicted a large grouping of Gram-negative Legionella pneumophila bacteria under 8000x magnification.<sup>Courtesy of Janice Haney Carr, Public Health Image Library<ref name="PHIL1">{{cite web |url=http://phil.cdc.gov/phil/details.asp |title=Public Health Image Library |last1=Haney Carr |first1=Janice |website= www.cdc.gov |access-date= Jan 15 2016}}</ref></sup>]] | ||
[[File:Legionella microscopy 2.jpg|thumb|none|Colorized scanning electron micrograph (SEM) depicted a large grouping of Gram-negative Legionella pneumophila bacteria under 10,000x magnification.<sup>Courtesy of Janice Haney Carr, Public Health Image Library<ref name=PHIL2>{{cite web |url=http://phil.cdc.gov/phil/details.asp |title=Public Health Image Library |last1=Haney Carr |first1=Janice |website= www.cdc.gov |access-date= Jan 15 2016}}</ref></sup>]] | [[File:Legionella microscopy 2.jpg|thumb|none|Colorized scanning electron micrograph (SEM) depicted a large grouping of Gram-negative Legionella pneumophila bacteria under 10,000x magnification.<sup>Courtesy of Janice Haney Carr, Public Health Image Library<ref name="PHIL2">{{cite web |url=http://phil.cdc.gov/phil/details.asp |title=Public Health Image Library |last1=Haney Carr |first1=Janice |website= www.cdc.gov |access-date= Jan 15 2016}}</ref></sup>]] | ||
[[File:Pittsburgh pneumonia Legionella micdadei.jpg|thumb|none|Legionella micdadei bacteria using TATLOCK conjugate in egg yolk sac suspension, DFA-stained photomicrograph with positive HEBA stain.<sup>Courtesy of Ann Herbert, Public Health Image Library<ref name=PHIL3>{{cite web |url=http://phil.cdc.gov/phil/details.asp |title=Public Health Image Library |last1=Herbert |first1=Anne |website= www.cdc.gov |access-date= Jan 15 2016}}</ref></sup>]] | [[File:Pittsburgh pneumonia Legionella micdadei.jpg|thumb|none|Legionella micdadei bacteria using TATLOCK conjugate in egg yolk sac suspension, DFA-stained photomicrograph with positive HEBA stain.<sup>Courtesy of Ann Herbert, Public Health Image Library<ref name="PHIL3">{{cite web |url=http://phil.cdc.gov/phil/details.asp |title=Public Health Image Library |last1=Herbert |first1=Anne |website= www.cdc.gov |access-date= Jan 15 2016}}</ref></sup>]] | ||
==References== | ==References== |
Revision as of 20:26, 13 June 2017
Legionellosis Microchapters |
Diagnosis |
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Treatment |
Case Studies |
Legionella pneumophila On the Web |
American Roentgen Ray Society Images of Legionella pneumophila |
Risk calculators and risk factors for Legionella pneumophila |
Legionella pneumophila | ||||||||||||||
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TEM image of L. pneumophila TEM image of L. pneumophila
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Scientific classification | ||||||||||||||
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Binomial name | ||||||||||||||
Legionella pneumophila Brenner DJ, Steigerwalt AG, McDade JE 1979 |
This page is about microbiologic aspects of the organism(s). For clinical aspects of the disease, see Legionellosis.
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
L.pneumophila is a ubiquitous aquatic organism that thrives in warm environments (32°- 45°C). L. pneumophila is a pleomorphic, aerobic, catalase-positive, oxidase-positive, non-spore-forming, non-capsulated, motile, Gram-negative bacteria. Although Legionella is categorized as a Gram-negative bacterium, it stains poorly to Gram stain due to its unique lipopolysaccharide-content in the outer psuedospamodulating leaflet of the outer cell membrane.
Microbiological Classification
Microbiological Characteristics
- L.pneumophila is a ubiquitous aquatic organism that thrives in warm environments (32°- 45°C).
- L. pneumophila is a pleomorphic, aerobic, catalase-positive, oxidase-positive, non-spore-forming, non-capsulated, motile, Gram-negative bacteria.
- Although Legionella is categorized as a Gram-negative bacterium, it stains poorly to Gram stain due to its unique lipopolysaccharide-content in the outer psuedospamodulating leaflet of the outer cell membrane.[1]
- Legionella is non-fermentative and is unable to hydrolyse gelatin or produce urease.
- At least 46 species and 70 serogroups of Legionella have been identified.
Life Cycle
- There are two major phases to the life cycle.
- The first is called the replicative phase. During this period, the bacteria are nonmotile and have a low toxicity.
- In the second phase, called the infectious phase, the bacteria are shorter and thicker.
Gallery
References
- ↑ Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed. ed.). McGraw Hill. ISBN 0-8385-8529-9.
- ↑ Haney Carr, Janice. "Public Health Image Library". www.cdc.gov. Retrieved Jan 15 2016. Check date values in:
|access-date=
(help) - ↑ Haney Carr, Janice. "Public Health Image Library". www.cdc.gov. Retrieved Jan 15 2016. Check date values in:
|access-date=
(help) - ↑ Herbert, Anne. "Public Health Image Library". www.cdc.gov. Retrieved Jan 15 2016. Check date values in:
|access-date=
(help)