Chronic myelogenous leukemia other diagnostic studies: Difference between revisions
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Genomic PCR and Southern blot assay can determine the exact breakpoints of the fusion genes. Reverse transcriptase (RT) PCR and Northern blot analysis detect BCR-ABL transcripts at the RNA level. Western blot analysis or immunoprecipitation demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl (27).PMID:10428738 PMID:20221270 | Genomic PCR and Southern blot assay can determine the exact breakpoints of the fusion genes. Reverse transcriptase (RT) PCR and Northern blot analysis detect BCR-ABL transcripts at the RNA level. Western blot analysis or immunoprecipitation demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl (27).PMID:10428738 PMID:20221270 | ||
Genomic PCR, Southern blot assay, Reverse transcriptase (RT) PCR, Northern blot analysis, Western blot analysis or immunoprecipitation can be helpful in the diagnosis of chronic myelogenous leukemia. | |||
==Other diagnostic studies== | ==Other diagnostic studies== | ||
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:*Used to detect any metastasis to the [[cerebral spinal fluid]] (CSF) | :*Used to detect any metastasis to the [[cerebral spinal fluid]] (CSF) | ||
*[[Lymph node biopsy]] | *[[Lymph node biopsy]] | ||
**Used to detect and/or determine the type of leukemic cells | |||
*Genomic PCR | |||
**can determine the exact breakpoints of the fusion genes | |||
*Southern blot assay | |||
**can determine the exact breakpoints of the fusion genes | |||
*Reverse transcriptase (RT) PCR | |||
**detect BCR-ABL transcripts at the RNA level | |||
*Northern blot analysis | |||
**detect BCR-ABL transcripts at the RNA level | |||
*Western blot analysis | |||
**demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl | |||
*[[Immunoprecipitation]] | |||
**demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl | |||
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==Gallery== | ==Gallery== | ||
<gallery widths="200px" class="center"> | <gallery widths="200px" class="center"> | ||
Revision as of 18:20, 19 May 2018
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Chronic myelogenous leukemia Microchapters |
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Differentiating Chronic myelogenous leukemia from other Diseases |
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Mohamad Alkateb, MBBCh [2]
Overview
Other diagnostic studies for chronic myelogenous leukemia include bone marrow aspiration and biopsy, lumbar puncture, and lymph node biopsy.
cytogenetic studies for Ph and markers of karyotypic evolution. In patients who are Ph negative, molecular studies, such as Southern blot assay or polymerase chain reaction (PCR), are indicated to detect the BCR-ABL rearrangements.PMID:10428738
Cytogenetic analysis is the gold standard diagnostic test in chronic myelogenous leukemia.PMID:10428738
Genomic PCR and Southern blot assay can determine the exact breakpoints of the fusion genes. Reverse transcriptase (RT) PCR and Northern blot analysis detect BCR-ABL transcripts at the RNA level. Western blot analysis or immunoprecipitation demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl (27).PMID:10428738 PMID:20221270
Genomic PCR, Southern blot assay, Reverse transcriptase (RT) PCR, Northern blot analysis, Western blot analysis or immunoprecipitation can be helpful in the diagnosis of chronic myelogenous leukemia.
Other diagnostic studies
Other diagnostic studies for chronic myelogenous leukemia include:[1]
- Used to detect and/or determine the type of leukemic cells
- Used to detect any metastasis to the cerebral spinal fluid (CSF)
- Lymph node biopsy
- Used to detect and/or determine the type of leukemic cells
- Genomic PCR
- can determine the exact breakpoints of the fusion genes
- Southern blot assay
- can determine the exact breakpoints of the fusion genes
- Reverse transcriptase (RT) PCR
- detect BCR-ABL transcripts at the RNA level
- Northern blot analysis
- detect BCR-ABL transcripts at the RNA level
- Western blot analysis
- demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl
- Immunoprecipitation
- demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl
Gallery
-
![Bone marrow aspiration and biopsy. After a small area of skin is numbed, a Jamshidi needle (a long, hollow needle) is inserted into the patient’s hip bone. Samples of blood, bone, and bone marrow are removed for examination under a microscope.[2]](/images/e/e4/CDR554337-274.jpg)
Bone marrow aspiration and biopsy. After a small area of skin is numbed, a Jamshidi needle (a long, hollow needle) is inserted into the patient’s hip bone. Samples of blood, bone, and bone marrow are removed for examination under a microscope.[2]
![Bone marrow aspiration and biopsy. After a small area of skin is numbed, a Jamshidi needle (a long, hollow needle) is inserted into the patient’s hip bone. Samples of blood, bone, and bone marrow are removed for examination under a microscope.[2]](/index.php/File:CDR554337-274.jpg)
References
- ↑ Canadian Cancer Society.2015.http://www.cancer.ca/en/cancer-information/cancer-type/leukemia-chronic-myelogenous-cml/diagnosis/?region=ab
- ↑ National Cancer Institute. Physician Data Query Database 2015.http://www.cancer.gov/types/leukemia/patient/cml-treatment-pdq