CAT box gene transcriptions: Difference between revisions
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The "5‘ flanking region of the rat acetylcholine receptor (AChR) ''β'' subunit gene [with] regulatory elements that confer muscle specificity [includes] a minimal TATA-box-less promoter region containing an initiator motif. An 85-bp fragment [promotes] high muscle-specific expression of a chloramphenicol acetyltransferase (CAT) reporter construct upon transfection in primary muscle cells. This sequence can be functionally dissected in a basal muscle-specific promoter element carrying a M-CAT box that is flanked at the 5’ end by an enhancer element with two binding sites for myogenic factors. Point mutations in the M-CAT box cause the loss of transcriptional activity of the basal promoter fragment. The enhancer activity depends on the presence of both E boxes that cooperate in a synergistic fashion. [The] control of muscle-specific and developmental expression of the rat AChR ''β'' subunit gene requires both regulatory elements, the M-CAT box and two adjacent E boxes, located in close proximity to each other."<ref name=Berberich/> | |||
The "minimal regulatory region of the 5’ flanking sequence contains E box elements that are defined by the nucleotides CANNTG [26, 271. E boxes are shown to provide binding sites for helix-loop-helix proteins of the MyoDl family including MyoDl [28], myogenin [29, 301, MRF4/ herculin [31] and myf5 [32]."<ref name=Berberich/> | |||
"Partial sequence of the 5' flanking region of the rat AChR ''β'' subunit gene [contains] putative E box element [CAGGTG], putative Sp1 element [GGGGCGGGT at -85 nts], putative Shue box element [CCCTGGCCTGG at -15 nts], M-CAT box element [GCGGCCTC at -8 nts]."<ref name=Berberich/> | |||
"Within the first 140bp of the 5’ flanking region the position and sequence of three other putative regulatory elements, the Spl [43, 44], M-CAT [34] and Shue box [45], are conserved between mouse and rat".<ref name=Berberich/> | |||
"The M-CAT consensus sequence [is] CATTCCT".<ref name=Berberich/> | |||
"A CAT-box-like element, GCCATT [34], adjacent to the GC-box, is conserved in the three promoters."<ref name=Berberich>{{ cite journal | |||
|author=Christof Berberich, Ingolf Dürr, Michael Koenen and Veit Witzemann | |||
|title=Two adjacent E box elements and a M‐CAT box are involved in the muscle‐specific regulation of the rat acetylcholine receptor β subunit gene | |||
|journal=European Journal of Biochemistry | |||
|date=September 1993 | |||
|volume=216 | |||
|issue=2 | |||
|pages=395-404 | |||
|url=https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1993.tb18157.x | |||
|arxiv= | |||
|bibcode= | |||
|doi=10.1111/j.1432-1033.1993.tb18157.x | |||
|pmid= | |||
|accessdate=27 December 2019 }}</ref> | |||
==Acknowledgements== | ==Acknowledgements== | ||
Revision as of 04:10, 28 December 2019
The "four cystatin genes [GeneID: 1469 CST1, GeneID: 1470 CST2, GeneID: 1471 CST3, and GeneID: 1472 CST4] contain the ATA-box sequence (ATAAA) in their 5'-flanking regions; however, the CAT-box sequence (CAT), a binding site of the transcription factor, CTF, is found only in the 5'-flanking region of the S-type cystatin genes."[1]
The "5‘ flanking region of the rat acetylcholine receptor (AChR) β subunit gene [with] regulatory elements that confer muscle specificity [includes] a minimal TATA-box-less promoter region containing an initiator motif. An 85-bp fragment [promotes] high muscle-specific expression of a chloramphenicol acetyltransferase (CAT) reporter construct upon transfection in primary muscle cells. This sequence can be functionally dissected in a basal muscle-specific promoter element carrying a M-CAT box that is flanked at the 5’ end by an enhancer element with two binding sites for myogenic factors. Point mutations in the M-CAT box cause the loss of transcriptional activity of the basal promoter fragment. The enhancer activity depends on the presence of both E boxes that cooperate in a synergistic fashion. [The] control of muscle-specific and developmental expression of the rat AChR β subunit gene requires both regulatory elements, the M-CAT box and two adjacent E boxes, located in close proximity to each other."[2]
The "minimal regulatory region of the 5’ flanking sequence contains E box elements that are defined by the nucleotides CANNTG [26, 271. E boxes are shown to provide binding sites for helix-loop-helix proteins of the MyoDl family including MyoDl [28], myogenin [29, 301, MRF4/ herculin [31] and myf5 [32]."[2]
"Partial sequence of the 5' flanking region of the rat AChR β subunit gene [contains] putative E box element [CAGGTG], putative Sp1 element [GGGGCGGGT at -85 nts], putative Shue box element [CCCTGGCCTGG at -15 nts], M-CAT box element [GCGGCCTC at -8 nts]."[2]
"Within the first 140bp of the 5’ flanking region the position and sequence of three other putative regulatory elements, the Spl [43, 44], M-CAT [34] and Shue box [45], are conserved between mouse and rat".[2]
"The M-CAT consensus sequence [is] CATTCCT".[2]
"A CAT-box-like element, GCCATT [34], adjacent to the GC-box, is conserved in the three promoters."[2]
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
Initial content for this page in some instances came from Wikiversity.
See also
References
- ↑ Eiichi Saitoh and Satoko Isemura (January 1, 1993). "Molecular Biology of Human Salivary Cysteine Proteinase Inhibitors" (PDF). Critical Reviews in Oral Biology and Medicine. 4 (3/4): 487–93. doi:10.1177/10454411930040033301. Retrieved 2013-06-28.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 Christof Berberich, Ingolf Dürr, Michael Koenen and Veit Witzemann (September 1993). "Two adjacent E box elements and a M‐CAT box are involved in the muscle‐specific regulation of the rat acetylcholine receptor β subunit gene". European Journal of Biochemistry. 216 (2): 395–404. doi:10.1111/j.1432-1033.1993.tb18157.x. Retrieved 27 December 2019.