Celiac disease pathophysiology: Difference between revisions
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Barley and rye also induce symptoms of coeliac disease.<ref name="Grain toxicity"/> A small minority of coeliac patients also react to oats.<ref>{{cite journal | author = Lundin K, Nilsen E, Scott H, Løberg E, Gjøen A, Bratlie J, Skar V, Mendez E, Løvik A, Kett K | title = Oats induced villous atrophy in coeliac disease | journal = Gut | volume = 52 | issue = 11 | pages = 1649–52 | year = 2003 | url = http://gut.bmjjournals.com/cgi/content/full/52/11/1649 | id = PMID 14570737}}</ref><ref>{{cite journal | author = Størsrud S, Olsson M, Arvidsson Lenner R, Nilsson L, Nilsson O, Kilander A | title = Adult coeliac patients do tolerate large amounts of oats | journal = Eur J Clin Nutr | volume = 57 | issue = 1 | pages = 163-9 | year = 2003 | id = PMID 12548312|url= http://www.nature.com/ejcn/journal/v57/n1/abs/1601525a.html}}</ref> Most probably oats produce symptoms due to cross contamination with other grains in the fields or in the distribution channels. There is at least one oat vendor, Gluten Free Oats®, that offers oats that can be considered SAFE for people who are gluten intolerant because they are tested to be below 10 parts per million (ppm) by the University of Nebraska FARRP Laboratory <ref>http://www.glutenfreeoats.com, http://www.farrp.org/, http://www.farrp.org/analysis.htm</ref>. Another vendor (McCann's) which, while not claiming to be gluten-free, points out that the risk of contamination from their Oats product is low due to the processes they use.<ref>{{cite web |url=http://www.mccanns.ie/pages/faq.html |title=McCann's FAQ |accessdate=2006-11-03 |year=2004 |publisher=Odlum Group |quote=we reckon that the level of non-oat grains to be less than 0.05% }}</ref> Other cereals, such as maize (corn), quinoa, millet, sorghum, rice are safe for a patient to consume. Other carbohydrate-rich foods such as potatoes and bananas do not contain gluten and do not trigger symptoms. | Barley and rye also induce symptoms of coeliac disease.<ref name="Grain toxicity"/> A small minority of coeliac patients also react to oats.<ref>{{cite journal | author = Lundin K, Nilsen E, Scott H, Løberg E, Gjøen A, Bratlie J, Skar V, Mendez E, Løvik A, Kett K | title = Oats induced villous atrophy in coeliac disease | journal = Gut | volume = 52 | issue = 11 | pages = 1649–52 | year = 2003 | url = http://gut.bmjjournals.com/cgi/content/full/52/11/1649 | id = PMID 14570737}}</ref><ref>{{cite journal | author = Størsrud S, Olsson M, Arvidsson Lenner R, Nilsson L, Nilsson O, Kilander A | title = Adult coeliac patients do tolerate large amounts of oats | journal = Eur J Clin Nutr | volume = 57 | issue = 1 | pages = 163-9 | year = 2003 | id = PMID 12548312|url= http://www.nature.com/ejcn/journal/v57/n1/abs/1601525a.html}}</ref> Most probably oats produce symptoms due to cross contamination with other grains in the fields or in the distribution channels. There is at least one oat vendor, Gluten Free Oats®, that offers oats that can be considered SAFE for people who are gluten intolerant because they are tested to be below 10 parts per million (ppm) by the University of Nebraska FARRP Laboratory <ref>http://www.glutenfreeoats.com, http://www.farrp.org/, http://www.farrp.org/analysis.htm</ref>. Another vendor (McCann's) which, while not claiming to be gluten-free, points out that the risk of contamination from their Oats product is low due to the processes they use.<ref>{{cite web |url=http://www.mccanns.ie/pages/faq.html |title=McCann's FAQ |accessdate=2006-11-03 |year=2004 |publisher=Odlum Group |quote=we reckon that the level of non-oat grains to be less than 0.05% }}</ref> Other cereals, such as maize (corn), quinoa, millet, sorghum, rice are safe for a patient to consume. Other carbohydrate-rich foods such as potatoes and bananas do not contain gluten and do not trigger symptoms. | ||
===HLA genetic typing=== | ===Genetics=== | ||
The vast majority of coeliac patients have one of two types of [[HLA DQ]].<ref name="pmid17785484">{{cite journal |author=Hadithi M, von Blomberg BM, Crusius JB, ''et al'' |title=Accuracy of serologic tests and HLA-DQ typing for diagnosing celiac disease |journal=Ann. Intern. Med. |volume=147 |issue=5 |pages=294–302 |year=2007 |pmid=17785484 |doi=|url=http://www.annals.org/cgi/content/full/147/5/294}}</ref> This [[gene]] is part of the [[major histocompatibility complex|MHC class II antigen-presenting receptor]] (also called the [[human leukocyte antigen]]) system and distinguishes cells between self and non-self for the purposes of the [[immune system]]. There are 7 HLA DQ variants (DQ2 and DQ4 through 9). Two of these variants—[[HLA-DQ2|DQ2]] and [[HLA-DQ8|DQ8]]—are associated with coeliac disease. The gene is located on the short arm of the [[Chromosome 6 (human)|sixth chromosome]], and as a result of the [[genetic linkage|linkage]] this [[locus (genetics)|locus]] has been labeled CELIAC1. | |||
Over 95% of coeliac patients have an isoform of DQ2 (encoded by DQA1*05 and DQB1*02 genes) and [[HLA-DQ8|DQ8]] (encoded by the [[haplotype]] DQA1*03:DQB1*0302), which is inherited in families. The reason these genes produce an increase in risk of coeliac disease is that the receptors formed by these genes bind to [[gliadin]] peptides more tightly than other forms of the antigen-presenting receptor. Therefore, these forms of the receptor are more likely to activate [[T cell|T lymphocytes]] and initiate the autoimmune process.<ref name=VanHeelWest/> | |||
[[Image:DQa2b5_da_gliadin.jpg|frame|left|DQ α<sup>5</sup>-β<sup>2</sup> -binding cleft with a deamidated gliadin peptide (yellow), modified from {{PDB|1S9V}}<ref>{{cite journal | author = Kim C, Quarsten H, Bergseng E, Khosla C, Sollid L | title = Structural basis for HLA-DQ2-mediated presentation of gluten epitopes in celiac disease | journal = Proc Natl Acad Sci U S A | volume = 101 | issue = 12 | pages = 4175–9 | year = 2004 | id = PMID 15020763}}</ref> ]] | |||
Most coeliac patients bear a two-gene [[HLA-DQ]] [[haplotype]] referred to as [[HLA-DQ2#DQ2.5|DQ2.5 haplotype]]. This haplotype is composed of 2 adjacent gene [[allele]]s, DQA1*0501 and [[HLA-DQ2#DQB1.2A0201|DQB1*0201]], which encode the two subunits, DQ α<sup>5</sup> and DQ β<sup>2</sup>. In most individuals, this DQ2.5 isoform is encoded by one of two [[Chromosome 6 (human)|chromosomes 6]] inherited from parents. Most coeliacs inherit only one copy of this DQ2.5 haplotype, while some inherit it from ''both'' parents; the latter are especially at risk for coeliac disease, as well as being more susceptible to severe complications.<ref name="pmid17190762">{{cite journal | author = Jores RD, Frau F, Cucca F, ''et al'' | title = HLA-DQB1*0201 homozygosis predisposes to severe intestinal damage in celiac disease | journal = Scand. J. Gastroenterol. | volume = 42 | issue = 1 | pages = 48-53 | year = 2007 | pmid = 17190762 | doi = 10.1080/00365520600789859}}</ref> Some individuals inherit DQ2.5 from one parent and portions of the haplotype ([[HLA-DQ|DQB1*02]] or DQA1*05) from the other parent, increasing risk. Less commonly, some individuals inherit the DQA1*05 allele from one parent and the DQB1*02 from the other parent, called a trans-haplotype association, and these individuals are at similar risk for coeliac disease as those with a single DQ2.5 bearing chromosome 6, but in this instance disease tends not to be familial. Among the 6% of European celiacs that do not have DQ2.5(cis or trans) or DQ8, 4% are DQ2 and 2% DQA1*05, 0.4% cannot be linked to DQ8, DQA1*05, or DQB1*02.<ref name="pmid12651074">{{cite journal |author=Karell K, Louka AS, Moodie SJ, ''et al'' |title=HLA types in celiac disease patients not carrying the DQA1*05-DQB1*02 (DQ2) heterodimer: results from the European Genetics Cluster on Celiac Disease |journal=Hum. Immunol. |volume=64 |issue=4 |pages=469-77 |year=2003 |pmid=12651074 |doi= |issn=}}</ref> | |||
The frequency of these genes varies geographically. DQ2.5 has high frequency in peoples of North and Western Europe (Basque Country, Ireland,<ref>{{cite journal | author = Michalski J, McCombs C, Arai T, Elston R, Cao T, McCarthy C, Stevens F | title = HLA-DR, DQ genotypes of celiac disease patients and healthy subjects from the West of Ireland | journal = Tissue Antigens | volume = 47 | issue = 2 | pages = 127-33 | year = 1996 | id = PMID 8851726}}</ref> with highest frequencies), portions of Africa, and is associated disease in India,<ref>{{cite journal |author=Kaur G, Sarkar N, Bhatnagar S, ''et al'' |title=Pediatric celiac disease in India is associated with multiple DR3-DQ2 haplotypes |journal=Hum. Immunol. |volume=63 |issue=8 |pages=677-82 |year=2002 |pmid=12121676 |doi=}}</ref> but is not found along portions of the West Pacific rim. DQ8, spread more globally than DQ2.5, is more prevalent from South and Central America (up to 90% [[phenotype]] frequency).<ref>{{cite journal | author = Layrisse Z, Guedez Y, Domínguez E, Paz N, Montagnani S, Matos M, Herrera F, Ogando V, Balbas O, Rodríguez-Larralde A | title = Extended HLA haplotypes in a Carib Amerindian population: the Yucpa of the Perija Range | journal = Hum Immunol | volume = 62 | issue = 9 | pages = 992–1000 | year = 2001 | id = PMID 11543901}}</ref> | |||
In addition to the CELIAC1 locus, CELIAC2 ([[Chromosome 5 (human)|5]]q31-q33 - IBD5 locus), CELIAC3 ([[Chromosome 2 (human)|2]]q33 -CTLA4 locus), CELIAC4 ([[Chromosome 19 (human)|19]]q13.1 - MYOIXB locus), have been linked to coeliac disease. The [[CTLA4]] and [[myosin IXB]] and gene have been found to be linked to coeliac disease and other autoimmune diseases.<ref name="pmid16025348">{{cite journal | author = Zhernakova A, Eerligh P, Barrera P, ''et al'' | title = CTLA4 is differentially associated with autoimmune diseases in the Dutch population | journal = Hum. Genet. | volume = 118 | issue = 1 | pages = 58-66 | year = 2005 | pmid = 16025348 | doi = 10.1007/s00439-005-0006-z}}</ref><ref name="pmid17584584">{{cite journal | author = Sánchez E, Alizadeh BZ, Valdigem G, ''et al'' | title = MYO9B gene polymorphisms are associated with autoimmune diseases in Spanish population | journal = Hum. Immunol. | volume = 68 | issue = 7 | pages = 610-5 | year = 2007 | pmid = 17584584 | doi = 10.1016/j.humimm.2007.03.006}}</ref> Two additional loci on [[Chromosome 4 (human)|chromosome 4]], 4q27 (IL2 or IL21 locus) and 4q14, have been found to be linked to coeliac disease.<ref name="pmid17558408">{{cite journal | author = van Heel DA, Franke L, Hunt KA, ''et al'' | title = A genome-wide association study for celiac disease identifies risk variants in the region harboring IL2 and IL21 | journal = Nat Genet | volume = 39 | issue = 7 | pages = 827-9 | year = 2007 | pmid = 17558408 | doi = 10.1038/ng2058}}</ref><ref>{{cite journal | author = Popat S, Bevan S, Braegger C, Busch A, O'Donoghue D, Falth-Magnusson K, Godkin A, Hogberg L, Holmes G, Hosie K, Howdle P, Jenkins H, Jewell D, Johnston S, Kennedy N, Kumar P, Logan R, Love A, Marsh M, Mulder C, Sjoberg K, Stenhammar L, Walker-Smith J, Houlston R | title = Genome screening of coeliac disease | journal = J Med Genet | volume = 39 | issue = 5 | pages = 328-31 | year = 2002 | url = http://jmg.bmjjournals.com/cgi/content/full/39/5/328 | id = PMID 12011149}}</ref> | |||
====HLA genetic typing==== | |||
Antibody testing and [[Human leukocyte antigen|HLA]] testing have similar accuracies<ref name="pmid17785484">{{cite journal |author=Hadithi M, von Blomberg BM, Crusius JB, ''et al.'' |title=Accuracy of serologic tests and HLA-DQ typing for diagnosing celiac disease |journal=[[Annals of Internal Medicine]] |volume=147 |issue=5 |pages=294–302 |year=2007 |month=September |pmid=17785484 |doi= |url=}}</ref> | Antibody testing and [[Human leukocyte antigen|HLA]] testing have similar accuracies<ref name="pmid17785484">{{cite journal |author=Hadithi M, von Blomberg BM, Crusius JB, ''et al.'' |title=Accuracy of serologic tests and HLA-DQ typing for diagnosing celiac disease |journal=[[Annals of Internal Medicine]] |volume=147 |issue=5 |pages=294–302 |year=2007 |month=September |pmid=17785484 |doi= |url=}}</ref> | ||
Revision as of 02:22, 31 August 2012
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
Celiac disease is caused by a reaction to gliadin, a gluten protein found in wheat (and similar proteins of the tribe Triticeae which includes other cultivars such as barley and rye). Upon exposure to gliadin, the enzyme tissue transglutaminase modifies the protein, and the immune system cross-reacts with the bowel tissue, causing an inflammatory reaction. That leads to flattening of the lining of the small intestine, which interferes with the absorption of nutrients. The only effective treatment is a lifelong gluten-free diet.
Pathophysiology
Coeliac disease appears to be polyfactorial, both in that more than one abnormal factor can cause the disease and also more than one factor is necessary for the disease to manifest in a patient.
Most all coeliac patients have abnormal HLA DQ2 allele. However, about 20–30% of people without coeliac disease have inherited an abnormal HLA-DQ2 allele. This suggests additional factors are needed for coeliac disease to develop. Furthermore, about 5% of those people who do develop coeliac disease do not have the DQ2 gene.
The HLA-DQ2 allele shows incomplete penetrance, as the gene alleles associated with the disease appear in most patients, but are neither present in all cases nor sufficient by themselves cause the disease.
Role of other grains
Wheat varieties or subspecies containing gluten such as spelt and Kamut®, and the rye/wheat hybrid triticale, also trigger symptoms.[1]
Barley and rye also induce symptoms of coeliac disease.[1] A small minority of coeliac patients also react to oats.[2][3] Most probably oats produce symptoms due to cross contamination with other grains in the fields or in the distribution channels. There is at least one oat vendor, Gluten Free Oats®, that offers oats that can be considered SAFE for people who are gluten intolerant because they are tested to be below 10 parts per million (ppm) by the University of Nebraska FARRP Laboratory [4]. Another vendor (McCann's) which, while not claiming to be gluten-free, points out that the risk of contamination from their Oats product is low due to the processes they use.[5] Other cereals, such as maize (corn), quinoa, millet, sorghum, rice are safe for a patient to consume. Other carbohydrate-rich foods such as potatoes and bananas do not contain gluten and do not trigger symptoms.
Genetics
The vast majority of coeliac patients have one of two types of HLA DQ.[6] This gene is part of the MHC class II antigen-presenting receptor (also called the human leukocyte antigen) system and distinguishes cells between self and non-self for the purposes of the immune system. There are 7 HLA DQ variants (DQ2 and DQ4 through 9). Two of these variants—DQ2 and DQ8—are associated with coeliac disease. The gene is located on the short arm of the sixth chromosome, and as a result of the linkage this locus has been labeled CELIAC1.
Over 95% of coeliac patients have an isoform of DQ2 (encoded by DQA1*05 and DQB1*02 genes) and DQ8 (encoded by the haplotype DQA1*03:DQB1*0302), which is inherited in families. The reason these genes produce an increase in risk of coeliac disease is that the receptors formed by these genes bind to gliadin peptides more tightly than other forms of the antigen-presenting receptor. Therefore, these forms of the receptor are more likely to activate T lymphocytes and initiate the autoimmune process.[7]
Most coeliac patients bear a two-gene HLA-DQ haplotype referred to as DQ2.5 haplotype. This haplotype is composed of 2 adjacent gene alleles, DQA1*0501 and DQB1*0201, which encode the two subunits, DQ α5 and DQ β2. In most individuals, this DQ2.5 isoform is encoded by one of two chromosomes 6 inherited from parents. Most coeliacs inherit only one copy of this DQ2.5 haplotype, while some inherit it from both parents; the latter are especially at risk for coeliac disease, as well as being more susceptible to severe complications.[9] Some individuals inherit DQ2.5 from one parent and portions of the haplotype (DQB1*02 or DQA1*05) from the other parent, increasing risk. Less commonly, some individuals inherit the DQA1*05 allele from one parent and the DQB1*02 from the other parent, called a trans-haplotype association, and these individuals are at similar risk for coeliac disease as those with a single DQ2.5 bearing chromosome 6, but in this instance disease tends not to be familial. Among the 6% of European celiacs that do not have DQ2.5(cis or trans) or DQ8, 4% are DQ2 and 2% DQA1*05, 0.4% cannot be linked to DQ8, DQA1*05, or DQB1*02.[10]
The frequency of these genes varies geographically. DQ2.5 has high frequency in peoples of North and Western Europe (Basque Country, Ireland,[11] with highest frequencies), portions of Africa, and is associated disease in India,[12] but is not found along portions of the West Pacific rim. DQ8, spread more globally than DQ2.5, is more prevalent from South and Central America (up to 90% phenotype frequency).[13]
In addition to the CELIAC1 locus, CELIAC2 (5q31-q33 - IBD5 locus), CELIAC3 (2q33 -CTLA4 locus), CELIAC4 (19q13.1 - MYOIXB locus), have been linked to coeliac disease. The CTLA4 and myosin IXB and gene have been found to be linked to coeliac disease and other autoimmune diseases.[14][15] Two additional loci on chromosome 4, 4q27 (IL2 or IL21 locus) and 4q14, have been found to be linked to coeliac disease.[16][17]
HLA genetic typing
Antibody testing and HLA testing have similar accuracies[6]
Pathology
The classic pathology changes of coeliac disease in the small bowel are categorized by the "Marsh classification":[18]
- Marsh stage 0: normal mucosa
- Marsh stage 1: increased number of intra-epithelial lymphocytes, usually exceeding 20 per 100 enterocytes
- Marsh stage 2: proliferation of the crypts of Lieberkuhn
- Marsh stage 3: partial or complete villous atrophy
- Marsh stage 4: hypoplasia of the small bowel architecture
The changes classically improve or reverse after gluten is removed from the diet, so many official guidelines recommend a repeat biopsy several (4–6) months after commencement of gluten exclusion.
In some cases a deliberate gluten challenge, followed by biopsy, may be conducted to confirm or refute the diagnosis. A normal biopsy and normal serology after challenge indicates the diagnosis may have been incorrect. Patients are warned that one does not "outgrow" coeliac disease in the same way as childhood food intolerances.
Associated Conditions
- Insulin dependent diabetes mellitus (IDDM)
- Sjogren’s syndrome
- Thyroid disease
- Dermatitis herpetiformis – extraintestinal manifestation
References
- ↑ 1.0 1.1 "Grain toxicity" (RTF). The CELIAC list. Retrieved 2006-08-27.
- ↑ Lundin K, Nilsen E, Scott H, Løberg E, Gjøen A, Bratlie J, Skar V, Mendez E, Løvik A, Kett K (2003). "Oats induced villous atrophy in coeliac disease". Gut. 52 (11): 1649–52. PMID 14570737.
- ↑ Størsrud S, Olsson M, Arvidsson Lenner R, Nilsson L, Nilsson O, Kilander A (2003). "Adult coeliac patients do tolerate large amounts of oats". Eur J Clin Nutr. 57 (1): 163–9. PMID 12548312.
- ↑ http://www.glutenfreeoats.com, http://www.farrp.org/, http://www.farrp.org/analysis.htm
- ↑ "McCann's FAQ". Odlum Group. 2004. Retrieved 2006-11-03.
we reckon that the level of non-oat grains to be less than 0.05%
- ↑ 6.0 6.1 Hadithi M, von Blomberg BM, Crusius JB; et al. (2007). "Accuracy of serologic tests and HLA-DQ typing for diagnosing celiac disease". Ann. Intern. Med. 147 (5): 294–302. PMID 17785484.
- ↑ Invalid
<ref>tag; no text was provided for refs namedVanHeelWest - ↑ Kim C, Quarsten H, Bergseng E, Khosla C, Sollid L (2004). "Structural basis for HLA-DQ2-mediated presentation of gluten epitopes in celiac disease". Proc Natl Acad Sci U S A. 101 (12): 4175–9. PMID 15020763.
- ↑ Jores RD, Frau F, Cucca F; et al. (2007). "HLA-DQB1*0201 homozygosis predisposes to severe intestinal damage in celiac disease". Scand. J. Gastroenterol. 42 (1): 48–53. doi:10.1080/00365520600789859. PMID 17190762.
- ↑ Karell K, Louka AS, Moodie SJ; et al. (2003). "HLA types in celiac disease patients not carrying the DQA1*05-DQB1*02 (DQ2) heterodimer: results from the European Genetics Cluster on Celiac Disease". Hum. Immunol. 64 (4): 469–77. PMID 12651074.
- ↑ Michalski J, McCombs C, Arai T, Elston R, Cao T, McCarthy C, Stevens F (1996). "HLA-DR, DQ genotypes of celiac disease patients and healthy subjects from the West of Ireland". Tissue Antigens. 47 (2): 127–33. PMID 8851726.
- ↑ Kaur G, Sarkar N, Bhatnagar S; et al. (2002). "Pediatric celiac disease in India is associated with multiple DR3-DQ2 haplotypes". Hum. Immunol. 63 (8): 677–82. PMID 12121676.
- ↑ Layrisse Z, Guedez Y, Domínguez E, Paz N, Montagnani S, Matos M, Herrera F, Ogando V, Balbas O, Rodríguez-Larralde A (2001). "Extended HLA haplotypes in a Carib Amerindian population: the Yucpa of the Perija Range". Hum Immunol. 62 (9): 992–1000. PMID 11543901.
- ↑ Zhernakova A, Eerligh P, Barrera P; et al. (2005). "CTLA4 is differentially associated with autoimmune diseases in the Dutch population". Hum. Genet. 118 (1): 58–66. doi:10.1007/s00439-005-0006-z. PMID 16025348.
- ↑ Sánchez E, Alizadeh BZ, Valdigem G; et al. (2007). "MYO9B gene polymorphisms are associated with autoimmune diseases in Spanish population". Hum. Immunol. 68 (7): 610–5. doi:10.1016/j.humimm.2007.03.006. PMID 17584584.
- ↑ van Heel DA, Franke L, Hunt KA; et al. (2007). "A genome-wide association study for celiac disease identifies risk variants in the region harboring IL2 and IL21". Nat Genet. 39 (7): 827–9. doi:10.1038/ng2058. PMID 17558408.
- ↑ Popat S, Bevan S, Braegger C, Busch A, O'Donoghue D, Falth-Magnusson K, Godkin A, Hogberg L, Holmes G, Hosie K, Howdle P, Jenkins H, Jewell D, Johnston S, Kennedy N, Kumar P, Logan R, Love A, Marsh M, Mulder C, Sjoberg K, Stenhammar L, Walker-Smith J, Houlston R (2002). "Genome screening of coeliac disease". J Med Genet. 39 (5): 328–31. PMID 12011149.
- ↑ Marsh MN (1992). "Gluten, major histocompatibility complex, and the small intestine. A molecular and immunobiologic approach to the spectrum of gluten sensitivity ('celiac sprue')". Gastroenterology. 102 (1): 330–54. PMID 1727768. Unknown parameter
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