Cutaneous leishmaniasis laboratory findings: Difference between revisions
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Revision as of 19:44, 21 November 2012
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
Laboratory Findings
Diagnosis is based on the characteristic appearance of non-healing raised, scaling lesions that may ulcerate and become secondarily infected with organisms such asStaphylococcus aureus, in someone who has returned from an endemic area.
Microscopy
Clinicians should consider CL in people with chronic (nonhealing) skin lesions who have been in areas where leishmaniasis is found. Laboratory confirmation of the diagnosis is achieved by detecting Leishmania parasites (or DNA) in infected tissue, through light-microscopic examination of stained specimens, culture techniques, or molecular methods.
Serology
CDC can assist in all aspects of the diagnostic evaluation. Identification of the Leishmania species can be important, particularly if more than one species is found where the patient traveled and if the species can have different clinical and prognostic implications. Serologic testing generally is not useful for CL but can provide supportive evidence for the diagnosis of ML.
PCR
The gold standard for diagnosis is PCR.[1]
References
- ↑ Reithinger R & Dujardin J-C (2007). "Molecular diagnosis of leishmaniasis: current status and future applications". J Clin Microbiol. 45 (1): 21&ndash, 25. doi:10.1128/JCM.02029-06.