Chikungunya laboratory findings: Difference between revisions
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==Laboratory Findings== | |||
Common laboratory tests for chikungunya include [[RT-PCR]], virus isolation, and serological tests. | |||
*Virus isolation provides the most definitive diagnosis, but takes one to two weeks for completion and must be carried out in [[biosafety level 3]] laboratories.<ref name="WHO diagnosis">{{cite web |url=http://www.searo.who.int/EN/Section10/Section2246_12902.htm |title=WHO — Laboratory Diagnosis of Chikungunya Fevers |work= |accessdate=2008-07-11}}</ref> The technique involves exposing specific [[cell line]]s to samples from whole blood and identifying chikungunya virus-specific responses. | |||
*RT-PCR using [[nested primer]] pairs is used to amplify several chikungunya-specific [[gene]]s from whole blood. Results can be determined in one to two days. | |||
*Serological diagnosis requires a larger amount of blood than the other methods, and uses an [[ELISA]] assay to measure chikungunya-specific [[IgM]] levels. Results require two to three days, and [[false positive]]s can occur with infection via other related viruses, such as [[o'nyong'nyong virus]] and [[Semliki Forest virus]]. | |||
==References== | ==References== | ||
Revision as of 01:13, 7 December 2012
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Laboratory Findings
Common laboratory tests for chikungunya include RT-PCR, virus isolation, and serological tests.
- Virus isolation provides the most definitive diagnosis, but takes one to two weeks for completion and must be carried out in biosafety level 3 laboratories.[1] The technique involves exposing specific cell lines to samples from whole blood and identifying chikungunya virus-specific responses.
- RT-PCR using nested primer pairs is used to amplify several chikungunya-specific genes from whole blood. Results can be determined in one to two days.
- Serological diagnosis requires a larger amount of blood than the other methods, and uses an ELISA assay to measure chikungunya-specific IgM levels. Results require two to three days, and false positives can occur with infection via other related viruses, such as o'nyong'nyong virus and Semliki Forest virus.
References
- ↑ "WHO — Laboratory Diagnosis of Chikungunya Fevers". Retrieved 2008-07-11.