Sporotrichosis laboratory findings
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Laboratory findings
- Sporotrichosis is a chronic disease with slow progression and often subtle symptoms. It is difficult to diagnose, as many other diseases share similar symptoms and therefore must be ruled out.
- While within human and animal tissues, S. schenckii exists in its yeast form.
- Varying in size and shape, these curved cells typically have 2-6 μm diameters with cigar-like buds offshooting from a narrow base.
- Growing on Sabouraud dextrose agar, most S. schenckii strains become evident after 4 days. At this point, some strains lack dark pigment, while others have been infiltrated with dark pigment from the start. Upon transfer to Brain-Heart Infusion (BHI) agar, and cultured for 7 days at 37°C, the S. schenckii strains undergo dimorphism, manifesting as creamy off-white to beige colored colonies.
- Patients with sporotrichosis will likely have antibodies against the fungus S. schenckii, however, due to variability in sensitivity and specificity, antibody identification may not be a reliable diagnosis for this disease. The confirming diagnosis remains culturing the fungus from the skin, sputum, synovial fluid, and cerebrospinal fluid.
- Cats with sporotrichosis are unique in that the exudate from their lesions may contain numerous organisms. This makes cytological evaluation of exudate a valuable diagnostic tool in this species. Exudate is pyogranulomatous and phagocytic cells may be packed with yeast forms. These are variable in size, but many are cigar-shaped.[1]
References
- ↑ Alvarado-Ramírez E, Torres-Rodríguez JM (2007). "In vitro susceptibility of Sporothrix schenckii to six antifungal agents determined using three different methods". Antimicrob Agents Chemother. 51 (7): 2420–3. doi:10.1128/AAC.01176-06. PMC 1913275. PMID 17438048.