Donovanosis laboratory findings
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Kalsang Dolma, M.B.B.S.[2]; Nate Michalak, B.A.
Overview
Laboratory Findings
Microscopy
- Identification of Donovan bodies in tissue smears indicates donovanosis as a strong diagnosis. Donovan bodies are sufficiently unique from other etiologic agents that parasitize macrophages.[1]
- Slides can be created using two methods:[2]
- Tissue smear: after cleaning ulcer surface with saline, a cotton swab is rolled over the ulcer and then rolled over a slide.
- Tissue biopsy: tissue can be obtained from lesion with punch biopsy, forceps, or scalpel and then crushed between two slides. The lesion is often friable but local anesthetic may be necessary.
- Slides are then stained with Giemsa, Leishman's, or Wright's stain to reveal intracellular Donovan bodies within monocytes that may or may not be capsulated.[2]
- Haematoloxylin and eosin are poor stains.
- If swabs are taken for other diagnostic tests, the smear for Donovan bodies should be taken first to ensure there is adequate material to detect the Donovan bodies.