SF3A1

Splicing factor 3a, subunit 1, 120kDa
File:PBB Protein SF3A1 image.jpg PDB rendering based on 1we7.
Available structures PDB Ortholog search: Template:Homologene2PDBe PDBe, Template:Homologene2uniprot RCSB List of PDB id codes 1we7​, 1zkh​, 2dt6​, 2dt7​
Identifiers
Symbols	SF3A1 ; PRP21; PRPF21; SAP114; SF3A120
External IDs	Template:OMIM5 Template:MGI HomoloGene: 4294
Gene ontology Molecular function Template:GNF GO Cellular component Template:GNF GO Template:GNF GO Biological process Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO
RNA expression pattern
File:PBB GE SF3A1 201356 at tn.png
File:PBB GE SF3A1 201357 s at tn.png
File:PBB GE SF3A1 216457 s at tn.png
More reference expression data
Orthologs
Template:GNF Ortholog box
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a

Splicing factor 3a, subunit 1, 120kDa, also known as SF3A1, is a human gene.

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Further reading

• Krämer A, Mulhauser F, Wersig C; et al. (1996). "Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae". RNA. 1 (3): 260–72. PMID 7489498.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Chiara MD, Champion-Arnaud P, Buvoli M; et al. (1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proc. Natl. Acad. Sci. U.S.A. 91 (14): 6403–7. PMID 8022796.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Rain JC, Tartakoff AM, Krämer A, Legrain P (1996). "Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution". RNA. 2 (6): 535–50. PMID 8718683.CS1 maint: Multiple names: authors list (link)
• Neubauer G, King A, Rappsilber J; et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. 1999. PMID 9847074.
• Krämer A, Grüter P, Gröning K, Kastner B (1999). "Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP". J. Cell Biol. 145 (7): 1355–68. PMID 10385517.CS1 maint: Multiple names: authors list (link)
• Dunham I, Shimizu N, Roe BA; et al. (1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.". Mol. Cell. 5 (5): 779–87. PMID 10882114.CS1 maint: Multiple names: authors list (link)
• Gunther M, Laithier M, Brison O (2000). "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Mol. Cell. Biochem. 210 (1–2): 131–42. PMID 10976766.CS1 maint: Multiple names: authors list (link)
• Ajuh P, Kuster B, Panov K; et al. (2001). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". EMBO J. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMID 11101529.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Suzuki Y, Tsunoda T, Sese J; et al. (2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes". Genome Res. 11 (5): 677–84. doi:10.1101/gr.164001. PMID 11337467.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Will CL, Schneider C, MacMillan AM; et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMID 11500380.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Nesic D, Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. 21 (19): 6406–17. PMID 11533230.
• Jurica MS, Licklider LJ, Gygi SR; et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. PMID 11991638.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Will CL, Urlaub H, Achsel T; et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. 21 (18): 4978–88. PMID 12234937.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Beausoleil SA, Jedrychowski M, Schwartz D; et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMID 15302935.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Nesic D, Tanackovic G, Krämer A (2005). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis". J. Cell. Sci. 117 (Pt 19): 4423–33. doi:10.1242/jcs.01308. PMID 15316075.CS1 maint: Multiple names: authors list (link)
• Lin KT, Lu RM, Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMID 15456888.CS1 maint: Multiple names: authors list (link)
• Collins JE, Wright CL, Edwards CA; et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biol. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMID 15461802.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)