Neutrophil cytosolic factor 4

Neutrophil cytosolic factor 4, 40kDa
File:PBB Protein NCF4 image.jpg PDB rendering based on 1h6h.
Available structures PDB Ortholog search: Template:Homologene2PDBe PDBe, Template:Homologene2uniprot RCSB List of PDB id codes 1h6h​, 1oey​, 1w6x​, 1w70​, 1z9q​, 2dyb​
Identifiers
Symbols	NCF4 ; MGC3810; NCF; P40PHOX; SH3PXD4
External IDs	Template:OMIM5 Template:MGI HomoloGene: 525
Gene ontology Molecular function Template:GNF GO Template:GNF GO Cellular component Template:GNF GO Template:GNF GO Template:GNF GO Biological process Template:GNF GO Template:GNF GO Template:GNF GO
RNA expression pattern
File:PBB GE NCF4 207677 s at tn.png
File:PBB GE NCF4 205147 x at tn.png
More reference expression data
Orthologs
Template:GNF Ortholog box
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a

Neutrophil cytosolic factor 4, 40kDa, also known as NCF4, is a human gene.

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Further reading

• Matute JD, Arias AA, Dinauer MC, Patiño PJ (2006). "p40phox: the last NADPH oxidase subunit". Blood Cells Mol. Dis. 35 (2): 291–302. doi:10.1016/j.bcmd.2005.06.010. PMID 16102984.CS1 maint: Multiple names: authors list (link)
• Jones JH (1977). "The essence of operating room nursing. Paramedical personnel". The Australasian nurses journal. 7 (1): 44–5, 63–4. PMID 243433.
• Leto TL, Adams AG, de Mendez I (1994). "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets". Proc. Natl. Acad. Sci. U.S.A. 91 (22): 10650–4. PMID 7938008.CS1 maint: Multiple names: authors list (link)
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. PMID 8125298.
• Tsunawaki S, Mizunari H, Nagata M; et al. (1994). "A novel cytosolic component, p40phox, of respiratory burst oxidase associates with p67phox and is absent in patients with chronic granulomatous disease who lack p67phox". Biochem. Biophys. Res. Commun. 199 (3): 1378–87. doi:10.1006/bbrc.1994.1383. PMID 8147882.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Wientjes FB, Hsuan JJ, Totty NF, Segal AW (1994). "p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains". Biochem. J. 296 ( Pt 3): 557–61. PMID 8280052.CS1 maint: Multiple names: authors list (link)
• Dusi S, Donini M, Rossi F (1996). "Mechanisms of NADPH oxidase activation: translocation of p40phox, Rac1 and Rac2 from the cytosol to the membranes in human neutrophils lacking p47phox or p67phox". Biochem. J. 314 ( Pt 2): 409–12. PMID 8670049.CS1 maint: Multiple names: authors list (link)
• Zhan S, Vazquez N, Zhan S; et al. (1996). "Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex". Blood. 88 (7): 2714–21. PMID 8839867.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Someya A, Nagaoka I, Nunoi H, Yamashita T (1997). "Translocation of guinea pig p40-phox during activation of NADPH oxidase". Biochim. Biophys. Acta. 1277 (3): 217–25. PMID 8982388.CS1 maint: Multiple names: authors list (link)
• Sathyamoorthy M, de Mendez I, Adams AG, Leto TL (1997). "p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain". J. Biol. Chem. 272 (14): 9141–6. PMID 9083043.CS1 maint: Multiple names: authors list (link)
• Grogan A, Reeves E, Keep N; et al. (1998). "Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils". J. Cell. Sci. 110 ( Pt 24): 3071–81. PMID 9365277.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Fuchs A, Bouin AP, Rabilloud T, Vignais PV (1997). "The 40-kDa component of the phagocyte NADPH oxidase (p40phox) is phosphorylated during activation in differentiated HL60 cells". Eur. J. Biochem. 249 (2): 531–9. PMID 9370364.CS1 maint: Multiple names: authors list (link)
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K; et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. PMID 9373149.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Bouin AP, Grandvaux N, Vignais PV, Fuchs A (1998). "p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process". J. Biol. Chem. 273 (46): 30097–103. PMID 9804763.CS1 maint: Multiple names: authors list (link)
• Grandvaux N, Grizot S, Vignais PV, Dagher MC (1999). "The Ku70 autoantigen interacts with p40phox in B lymphocytes". J. Cell. Sci. 112 ( Pt 4): 503–13. PMID 9914162.CS1 maint: Multiple names: authors list (link)
• Nishiyama A, Ohno T, Iwata S; et al. (1999). "Demonstration of the interaction of thioredoxin with p40phox, a phagocyte oxidase component, using a yeast two-hybrid system". Immunol. Lett. 68 (1): 155–9. PMID 10397171.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Hasebe T, Someya A, Nagaoka I (1999). "Identification of a splice variant mRNA of p40phox, an NADPH oxidase component of phagocytes". FEBS Lett. 455 (3): 257–61. PMID 10437784.CS1 maint: Multiple names: authors list (link)
• Dunham I, Shimizu N, Roe BA; et al. (1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Vergnaud S, Paclet MH, El Benna J; et al. (2000). "Complementation of NADPH oxidase in p67-phox-deficient CGD patients p67-phox/p40-phox interaction". Eur. J. Biochem. 267 (4): 1059–67. PMID 10672014.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Grizot S, Grandvaux N, Fieschi F; et al. (2001). "Small angle neutron scattering and gel filtration analyses of neutrophil NADPH oxidase cytosolic factors highlight the role of the C-terminal end of p47phox in the association with p40phox". Biochemistry. 40 (10): 3127–33. PMID 11258927.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)