Sperm flagellar protein 2 is a protein that in humans is encoded by the SPEF2gene.[1][2][3][4]
SPEF2 plays an important role in spermatogenesis and flagellar assembly.[5] SPEF2 is expressed in all ciliated cells and is required for cilia function.[5][6] Sperm contain cilia, and a mutation in the SPEF2 gene can cause male infertility due to immobile sperm.[5][6] In a pig animal model, a SPEF2 mutation affects the sperm tail development.[6] And a loss of function mutation in SPEF2 in mice causes the big giant head phenotype.[5] SPEF2 mRNA and protein products are localized in germ and sertoli cells.[6] Within these cells, SPEF2 is localized in the golgi complex, manchette, basal body and mid piece of the sperm tail.[6] SPEF2 has been shown to interact with the intracellular transport protein IFT20 in the testis.[6]
References
↑Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (December 2000). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 7 (6): 347–55. doi:10.1093/dnares/7.6.347. PMID11214970.
↑ 6.06.16.26.36.46.5Sironen A, Hansen J, Thomsen B, Andersson M, Vilkki J, Toppari J, Kotaja N (March 2010). "Expression of SPEF2 during mouse spermatogenesis and identification of IFT20 as an interacting protein". Biology of Reproduction. 82 (3): 580–90. doi:10.1095/biolreprod.108.074971. PMID19889948.
Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
