RiboGreen

WikiDoc Resources for RiboGreen
Articles
Most recent articles on RiboGreen Most cited articles on RiboGreen Review articles on RiboGreen Articles on RiboGreen in N Eng J Med, Lancet, BMJ
Media
Powerpoint slides on RiboGreen Images of RiboGreen Photos of RiboGreen Podcasts & MP3s on RiboGreen Videos on RiboGreen
Evidence Based Medicine
Cochrane Collaboration on RiboGreen Bandolier on RiboGreen TRIP on RiboGreen
Clinical Trials
Ongoing Trials on RiboGreen at Clinical Trials.gov Trial results on RiboGreen Clinical Trials on RiboGreen at Google
Guidelines / Policies / Govt
US National Guidelines Clearinghouse on RiboGreen NICE Guidance on RiboGreen NHS PRODIGY Guidance FDA on RiboGreen CDC on RiboGreen
Books
Books on RiboGreen
News
RiboGreen in the news Be alerted to news on RiboGreen News trends on RiboGreen
Commentary
Blogs on RiboGreen
Definitions
Definitions of RiboGreen
Patient Resources / Community
Patient resources on RiboGreen Discussion groups on RiboGreen Patient Handouts on RiboGreen Directions to Hospitals Treating RiboGreen Risk calculators and risk factors for RiboGreen
Healthcare Provider Resources
Symptoms of RiboGreen Causes & Risk Factors for RiboGreen Diagnostic studies for RiboGreen Treatment of RiboGreen
Continuing Medical Education (CME)
CME Programs on RiboGreen
International
RiboGreen en Espanol RiboGreen en Francais
Business
RiboGreen in the Marketplace Patents on RiboGreen
Experimental / Informatics
List of terms related to RiboGreen

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RiboGreen is a proprietary fluorescent dye that is used in the detection and quantitation of nucleic acids, including both DNA and RNA. It is synthesized and marketed by Molecular Probes (a division of Invitrogen) of Eugene, Oregon, United States. In its free form, RiboGreen exhibits little fluorescence and possesses a negligible absorbance signature. When bound to nucleic acids, the dye fluoresces with an intensity that, according to the manufacturer, is several orders of magnitude greater than the unbound form. The fluorescence can be detected by a sensor and the nucleic acid can be quantified. The presence of protein contaminants in the sample of nucleic acids to be tested does not make significant contributions to the absorbance, and thus allows for the addition of deoxyribonucleases to the protocol in order to degrade DNA, in the instances where one is only interested in detecting or quantifying RNA.